Protocol to quantify total intracellular magnesium in small samples using a fluorescent plate reader and fluorescent dye.
Anconelli L, Farruggia G, Lombardo M, Rossi F, Rydzyk MM, Rossi M, Malucelli E, Cappadone C, Maier JA, Iotti S.
Anconelli L, et al.
STAR Protoc. 2025 Sep 19;6(3):103956. doi: 10.1016/j.xpro.2025.103956. Epub 2025 Jul 21.
STAR Protoc. 2025.
PMID: 40694473
Free PMC article.
Here, we present a protocol to quantify total intracellular Mg in samples with as few as several thousand cells using a fluorescent plate reader and the fluorescent dye diaza-18-crown-6-hydroxyquinoline-5 (DCHQ5). We describe steps for reagent setup, sample preparat …
Here, we present a protocol to quantify total intracellular Mg in samples with as few as several thousand cells using a fluorescent plate re …