Expression and Functional Analysis of the Compact Thermophilic Anoxybacillus flavithermus Cas9 Nuclease.
Matveeva A, Ryabchenko A, Petrova V, Prokhorova D, Zhuravlev E, Zakabunin A, Tikunov A, Stepanov G.
Matveeva A, et al.
Int J Mol Sci. 2023 Dec 4;24(23):17121. doi: 10.3390/ijms242317121.
Int J Mol Sci. 2023.
PMID: 38069443
Free PMC article.
This study focuses on generating a construct to express a compact Cas9 nuclease (AnoCas9) from the thermophilic microorganism Anoxybacillus flavithermus displaying the nuclease activity in the 37-60 C range and the PAM preference of 5'-NNNNCDAA-3' in vitro. ...
This study focuses on generating a construct to express a compact Cas9 nuclease (AnoCas9) from the thermophilic microorganism Anoxybacill …