Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation

Search Page

MyNCBI Filters
Results by year

Table representation of search results timeline featuring number of search results per year.

Year Number of Results
2010 1
2011 3
2012 5
2013 13
2014 9
2015 7
2016 6
2017 5
2018 3
2019 4
2020 0
Text availability
Article attribute
Article type
Publication date

Search Results

48 results
Results by year
Filters applied: . Clear all
Page 1
Proteomic mapping of mitochondria in living cells via spatially restricted enzymatic tagging
Rhee HW, et al. Science 2013. PMID 23371551 Free PMC article.
Our method relies on a genetically targetable peroxidase enzyme that biotinylates nearby proteins, which are subsequently purified and identified by MS. ...The specificity of peroxidase-mediated proteomic mapping in live cells, combined with its ease of use, offers biologists a powerful tool for understanding the molecular composition of living cells....
Our method relies on a genetically targetable peroxidase enzyme that biotinylates nearby proteins, which are subsequently purified an …
Ultrastructural localisation of protein interactions using conditionally stable nanobodies
Ariotti N, et al. PLoS Biol 2018. PMID 29621251 Free PMC article.
Conditionally stable GFP- and mCherry-binding nanobodies (termed csGBP and csChBP, respectively) are characterized using a cell-free expression and analysis system and subsequently fused to an ascorbate peroxidase (APEX) enzyme. ...
Conditionally stable GFP- and mCherry-binding nanobodies (termed csGBP and csChBP, respectively) are characterized using a cell-free express …
Electron microscopy using the genetically encoded APEX2 tag in cultured mammalian cells
Martell JD, et al. Nat Protoc 2017. PMID 28796234 Free PMC article.
We recently reported enhanced ascorbate peroxidase 2 (APEX2) as a broadly applicable genetic tag that generates EM contrast on a specific protein or subcellular compartment of interest. ...
We recently reported enhanced ascorbate peroxidase 2 (APEX2) as a broadly applicable genetic tag that generates EM contrast on …
Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy.
Martell JD, et al. Nat Biotechnol 2012. PMID 23086203 Free PMC article.
APEX is a monomeric 28-kDa peroxidase that withstands strong EM fixation to give excellent ultrastructural preservation. We demonstrate the utility of APEX for high-resolution EM imaging of a variety of mammalian organelles and specific proteins using a simple and robust labeling procedure. ...
APEX is a monomeric 28-kDa peroxidase that withstands strong EM fixation to give excellent ultrastructural preservation. We demonstra …
Isolation, purification and characterization of an ascorbate peroxidase from celery and overexpression of the AgAPX1 gene enhanced ascorbate content and drought tolerance in Arabidopsis.
Liu JX, et al. BMC Plant Biol 2019. PMID 31711410 Free PMC article.
BACKGROUND: Celery is a widely cultivated vegetable abundant in ascorbate (AsA), a natural plant antioxidant capable of scavenging free radicals generated by abiotic stress in plants. Ascorbate peroxidase (APX) is a plant antioxidant enzyme that is important in the synthesis of AsA and scavenging of excess hydrogen peroxide. ...
BACKGROUND: Celery is a widely cultivated vegetable abundant in ascorbate (AsA), a natural plant antioxidant capable of scavenging fr …
The knockdown of chloroplastic ascorbate peroxidases reveals its regulatory role in the photosynthesis and protection under photo-oxidative stress in rice.
Caverzan A, et al. Plant Sci 2014. PMID 24268165
The inactivation of the chloroplast ascorbate peroxidases (chlAPXs) has been thought to limit the efficiency of the water-water cycle and photo-oxidative protection under stress conditions. ...
The inactivation of the chloroplast ascorbate peroxidases (chlAPXs) has been thought to limit the efficiency of the water-wate …
Proteomic mapping in live Drosophila tissues using an engineered ascorbate peroxidase.
Chen CL, et al. Proc Natl Acad Sci U S A 2015. PMID 26362788 Free PMC article.
We established a proteomic mapping platform in live Drosophila tissues using an engineered ascorbate peroxidase (APEX). Upon activation, the APEX enzyme catalyzes the biotinylation of neighboring endogenous proteins that can then be isolated and identified by mass spectrometry. ...
We established a proteomic mapping platform in live Drosophila tissues using an engineered ascorbate peroxidase (APEX). Upon a …
The synthesis of cytosolic ascorbate peroxidases in germinating seeds and seedlings of soybean and their behavior under flooding stress.
Nishizawa K, et al. Biosci Biotechnol Biochem 2013. PMID 24200779 Free article.
Cytosolic ascorbate peroxidases (cAPXs) of soybean have been found by proteome analysis to be downregulated in submerged seedlings. ...
Cytosolic ascorbate peroxidases (cAPXs) of soybean have been found by proteome analysis to be downregulated in submerged seedl …
Genome-wide investigation and expression profiling of APX gene family in Gossypium hirsutum provide new insights in redox homeostasis maintenance during different fiber development stages
Tao C, et al. Mol Genet Genomics 2018. PMID 29307114 Free PMC article.
Ascorbate peroxidase (APX) is a member of heme-containing peroxidases which catalyze the H(2)O(2)-dependent oxidation of a wide range of substrates in plants and animals. ...Our previous work reported that ascorbate peroxidase 1 (GhAPX1) was important for cotton fiber elongation. However, knowledge about APX gene family members and their evolutionary and functional characteristics in cotton is limited. ...
Ascorbate peroxidase (APX) is a member of heme-containing peroxidases which catalyze the H(2)O(2)-dependent oxidation o
48 results
Jump to page
Feedback