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1997 30
1998 33
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2007 30
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Page 1
Southern blotting.
Brown T. Curr Protoc Immunol 2001 - Review. PMID 18432697
Southern blotting is the transfer of DNA fragments from an electrophoresis gel to a membrane support, resulting in immobilization of the DNA fragments, so the membrane carries a semipermanent reproduction of the banding pattern of the gel. ...This unit describes Southern blotting via upward capillary transfer of DNA from an agarose gel onto a nylon or nitrocellulose membrane, and subsequent immobilization by UV irradiation (for nylon) or baking (for nitrocellulose). ...
Southern blotting is the transfer of DNA fragments from an electrophoresis gel to a membrane support, resulting in immobilizat
Southern blotting.
Southern E. Nat Protoc 2006. PMID 17406277
This protocol describes a basic method to perform the Southern blot. Blotting allows the detection of specific molecules among a mixture separated by gel electrophoresis. ...
This protocol describes a basic method to perform the Southern blot. Blotting allows the detection of specific molecules among …
Southern blotting.
Brown T. Curr Protoc Protein Sci 2001. PMID 18429082
This appendix describes Southern blotting via upward capillary transfer of DNA from an agarose gel onto a nylon or nitrocellulose membrane, using a high-salt transfer buffer to promote binding of DNA to the membrane. ...Although the ease and reliability of capillary transfer methods makes this far and away the most popular system for Southern blotting with agarose gels, it unfortunately does not work with polyacrylamide gels, whose smaller pore size impedes the transverse movement of the DNA molecules. ...
This appendix describes Southern blotting via upward capillary transfer of DNA from an agarose gel onto a nylon or nitrocellul …
Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction.
Zhou D, et al. J Vis Exp 2018. PMID 30531726
To obtain ideal results, the probes and restriction enzyme (RE) cutting sites for Southern blotting and the primers for PCR should be carefully planned. Though the execution of Southern blotting is time-consuming and labor-intensive and PCR results have false positives, the correct identification by Southern blotting and the rapid screening by PCR allow the sole or combined application of these methods described in this paper to be widely used and consulted by most labs in the identification of genotypes of ES cells and genetically modified animals....
To obtain ideal results, the probes and restriction enzyme (RE) cutting sites for Southern blotting and the primers for PCR sh …
Genomic Southern blot analysis.
Gebbie L. Methods Mol Biol 2014. PMID 24243203
This chapter describes a detailed protocol for genomic Southern blot analysis which can be used to detect transgene or endogenous gene sequences in cereal genomes. ...High sensitivity and limited background are key to successful Southern blots. The critical steps in this protocol are complete digestion of the right quantity of DNA, careful handling of the membrane to avoid unnecessary background, and optimization of probe concentration and temperatures during the hybridization step. ...
This chapter describes a detailed protocol for genomic Southern blot analysis which can be used to detect transgene or endogenous gen …
Inverse PCR and Quantitative PCR as Alternative Methods to Southern Blotting Analysis to Assess Transgene Copy Number and Characterize the Integration Site in Transgenic Woody Plants.
Stefano B, et al. Biochem Genet 2016. PMID 26895172
After assessing the transgene copy number of a set of apple transgenic clones with Southern blotting, we describe two alternative methods: the first is based on inverse PCR (i-PCR) and the second on the quantitative PCR (q-PCR). The methods produced comparable results with the exception of the data regarding a high copy number clone, but while the q-PCR-based system is rapid and easily adaptable to high throughput systems, the i-PCR-based method can provide information regarding the transformation event and the characteristics of the sequences flanking the transgenic construct....
After assessing the transgene copy number of a set of apple transgenic clones with Southern blotting, we describe two alternat …
Two-dimensional agarose gel electrophoresis for analysis of DNA replication
Villwock SK and Aparicio OM. Methods Mol Biol 2014. PMID 25213253
This method involves: isolation of genomic DNA while preserving fragile replication structures, digestion of the DNA with a restriction enzyme, separation of DNA by size and shape through two distinct stages of agarose gel electrophoresis, and Southern blotting to probe for the specific sequence(s) of interest. ...
This method involves: isolation of genomic DNA while preserving fragile replication structures, digestion of the DNA with a restriction enzy …
Huntington Disease: Molecular Diagnostics Approach
Bastepe M and Xin W. Curr Protoc Hum Genet 2015. PMID 26439718
This unit includes protocols that describe the complementary use of polymerase chain reactions (PCR) and Southern blot hybridization to accurately measure the CAG trinucleotide repeat size and interpret the test results. ...
This unit includes protocols that describe the complementary use of polymerase chain reactions (PCR) and Southern blot hybridization …
Validation of a Screening Method for Dynamic Mutations in the FMR1 Gene
Škrlec I, et al. Ann Clin Lab Sci 2018. PMID 30610057
The objectives of this study were to validate the direct triplet-primed PCR method (dTP-PCR) for determination of dynamic mutations in the FMR1 gene, and to compare the results of the dTP-PCR method and Southern blot analysis. ...
The objectives of this study were to validate the direct triplet-primed PCR method (dTP-PCR) for determination of dynamic mutations in the F …
Analysis of DNA by Southern blotting.
Glenn G and Andreou LV. Methods Enzymol 2013. PMID 24011036
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