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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1982 1
1985 3
1986 3
1987 22
1988 631
1989 1999
1990 2283
1991 3306
1992 3406
1993 2833
1994 2800
1995 2992
1996 3137
1997 3275
1998 3190
1999 3940
2000 4911
2001 6118
2002 6320
2003 6954
2004 7553
2005 8578
2006 7704
2007 7316
2008 7706
2009 9114
2010 11483
2011 11358
2012 9865
2013 9514
2014 8155
2015 7546
2016 5902
2017 3796
2018 1973
2019 841
2020 36
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160,798 results
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Page 1
Protein purification and analysis: next generation Western blotting techniques.
Mishra M, et al. Expert Rev Proteomics 2017 - Review. PMID 28974114 Free PMC article.
The review discusses the most advanced western blotting techniques available and highlights the relationship between next generation western blotting techniques and its clinical relevance. ...New methods such as single cell-resolution western blot, capillary electrophoresis, DigiWest, automated microfluid western blotting and microchip electrophoresis have all been developed to reduce potential problems associated with the western blotting technique. ...
The review discusses the most advanced western blotting techniques available and highlights the relationship between next gene …
Western blotting: an introduction.
Kurien BT and Scofield RH. Methods Mol Biol 2015 - Review. PMID 26043986
Western blotting is an important procedure for the immunodetection of proteins, particularly proteins that are of low abundance. ...Protein blotting has evolved greatly since the inception of this protocol, allowing protein transfer to be accomplished in a variety of ways....
Western blotting is an important procedure for the immunodetection of proteins, particularly proteins that are of low abundanc
Western blotting.
Kurien BT and Scofield RH. Methods 2006. PMID 16483794
Western blotting (protein blotting or immunoblotting) is a powerful and important procedure for the immunodetection of proteins post-electrophoresis, particularly proteins that are of low abundance. Since the inception of the protocol for protein transfer from an electrophoresed gel to a membrane in 1979, protein blotting has evolved greatly. ...
Western blotting (protein blotting or immunoblotting) is a powerful and important procedure for the immunodetection of
The design of a quantitative western blot experiment.
Taylor SC and Posch A. Biomed Res Int 2014 - Review. PMID 24738055 Free PMC article.
Western blotting is a technique that has been in practice for more than three decades that began as a means of detecting a protein target in a complex sample. ...We have recently published an updated approach to produce quantitative densitometric data from western blots (Taylor et al., 2013) and here we summarize the complete western blot workflow with a focus on sample preparation and data analysis for quantitative western blotting....
Western blotting is a technique that has been in practice for more than three decades that began as a means of detecting a pro
The Western Blot.
Hnasko TS and Hnasko RM. Methods Mol Biol 2015. PMID 26160567
Western blotting is a technique that involves the separation of proteins by gel electrophoresis, their blotting or transfer to a membrane, and selective immunodetection of an immobilized antigen. ...
Western blotting is a technique that involves the separation of proteins by gel electrophoresis, their blotting or tran
Single cell-resolution western blotting.
Kang CC, et al. Nat Protoc 2016. PMID 27466711 Free PMC article.
This protocol describes how to perform western blotting on individual cells to measure cell-to-cell variation in protein expression levels and protein state. Like conventional western blotting, single-cell western blotting (scWB) is particularly useful for protein targets that lack selective antibodies (e.g., isoforms) and in cases in which background signal from intact cells is confounding. scWB is performed on a microdevice that comprises an array of microwells molded in a thin layer of a polyacrylamide gel (PAG). ...
This protocol describes how to perform western blotting on individual cells to measure cell-to-cell variation in protein expre …
An overview of technical considerations for Western blotting applications to physiological research.
Bass JJ, et al. Scand J Med Sci Sports 2017 - Review. PMID 27263489 Free PMC article.
The applications of Western/immunoblotting (WB) techniques have reached multiple layers of the scientific community and are now considered routine procedures in the field of physiology. ...The present review aims to provide a detailed description and critique of WB procedures and technicalities, from sample collection through preparation, blotting and detection, to analysis of the data collected. ...
The applications of Western/immunoblotting (WB) techniques have reached multiple layers of the scientific community and are now consi …
Single-cell western blotting.
Hughes AJ, et al. Nat Methods 2014. PMID 24880876 Free PMC article.
To measure cell-to-cell variation in protein-mediated functions, we developed an approach to conduct ∼10(3) concurrent single-cell western blots (scWesterns) in ∼4 h. A microscope slide supporting a 30-μm-thick photoactive polyacrylamide gel enables western blotting: settling of single cells into microwells, lysis in situ, gel electrophoresis, photoinitiated blotting to immobilize proteins and antibody probing. ...
To measure cell-to-cell variation in protein-mediated functions, we developed an approach to conduct ∼10(3) concurrent single-cell wester
Western Blot Techniques.
Kim B. Methods Mol Biol 2017. PMID 28501998
The Western blot is an important laboratory technique that allows for specific identification and characterization of proteins. ...
The Western blot is an important laboratory technique that allows for specific identification and characterization of proteins. ...
Antibody Validation by Western Blotting.
Signore M, et al. Methods Mol Biol 2017. PMID 28501993
In the simplest iteration, antibody specificity is determined by the presence of a single band in a complex biological sample, at the expected molecular weight, on a Western blot.To date, numerous Western blotting procedures are available, based on either manual or automated systems and spanning the spectrum of single blots to multiplex blots. ...This chapter focuses on the chemiluminescent detection of proteins using a manual Western blotting system and a vacuum-enhanced detection system (SNAP i.d.™, Millipore)....
In the simplest iteration, antibody specificity is determined by the presence of a single band in a complex biological sample, at the expect …
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