Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation

Search Page

MyNCBI Filters
Results by year

Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1956 1
1958 1
1970 2
1972 1
1976 3
1977 2
1979 1
1980 4
1985 1
1987 1
1988 2
1990 2
1991 3
1992 112
1993 412
1994 466
1995 498
1996 461
1997 559
1998 566
1999 596
2000 577
2001 620
2002 592
2003 570
2004 535
2005 606
2006 592
2007 550
2008 586
2009 642
2010 676
2011 705
2012 771
2013 734
2014 670
2015 650
2016 630
2017 601
2018 564
2019 235
2020 2
Text availability
Article attribute
Article type
Publication date

Search Results

14,482 results
Results by year
Filters applied: . Clear all
Page 1
Isolation and Characterization of Functionally Active Extracellular Vesicles from Culture Medium Conditioned by Bovine Embryos In Vitro.
Pavani KC, et al. Int J Mol Sci 2018. PMID 30577682 Free PMC article.
Initially, ultracentrifuged bovine serum albumin (BSA) containing medium was selected as suitable EV-free embryo culture medium. ...Since BSA did not appear to be a contaminating EV source in culture medium, EV functionality was tested in BSA containing medium. ...
Initially, ultracentrifuged bovine serum albumin (BSA) containing medium was selected as suitable EV-free embryo culture me
Directed Differentiation of Human Corneal Endothelial Cells From Human Embryonic Stem Cells by Using Cell-Conditioned Culture Media.
Chen X, et al. Invest Ophthalmol Vis Sci 2018. PMID 30025120
METHODS: HESC are used to differentiate into HCEC by employing a two-stage method, which involves the application of two different types of conditioned culture medium, human corneal stromal cell-conditioned medium (HCSC-CM) and lens epithelial cell (LEC) plus HCSC-CM (LEC-CM+HCEC-CM). In brief, hESCs were treated with different conditioned media to induce directed endothelial differentiation. RESULTS: In the presence of conditioned culture medium, embryonic stem cells differentiate first under the control of periocular mesenchymal precursors (POMPs). ...
METHODS: HESC are used to differentiate into HCEC by employing a two-stage method, which involves the application of two diffe
Exosomes from Cell Culture-Conditioned Medium: Isolation by Ultracentrifugation and Characterization.
Purushothaman A. Methods Mol Biol 2019. PMID 30825179
Exosomes have been purified from in vitro cell culture supernatants by many different methods; however the most simple and reliable method involves purification by ultracentrifugation. This chapter describes a detailed protocol for isolating exosomes from cell culture-conditioned medium using ultracentrifugation and their characterization based upon size, number, and protein expression by several complementary methods such as transmission electron microscopy, nanoparticle tracking analysis, western blotting, and flow cytometry....
Exosomes have been purified from in vitro cell culture supernatants by many different methods; however the most simple and rel …
Mesenchymal Stromal Cell Secretome: Influencing Therapeutic Potential by Cellular Pre-conditioning.
Ferreira JR, et al. Front Immunol 2018 - Review. PMID 30564236 Free PMC article.
Mesenchymal stromal cells (MSCs) are self-renewing, culture-expandable adult stem cells that have been isolated from a variety of tissues, and possess multipotent differentiation capacity, immunomodulatory properties, and are relatively non-immunogenic. Due to this unique set of characteristics, these cells have attracted great interest in the field of regenerative medicine and have been shown to possess pronounced therapeutic potential in many different pathologies. ...
Mesenchymal stromal cells (MSCs) are self-renewing, culture-expandable adult stem cells that have been isolated from a variety
An Effect of Culture Media on Epithelial Differentiation Markers in Breast Cancer Cell Lines MCF7, MDA-MB-436 and SkBr3.
Pirsko V, et al. Medicina (Kaunas) 2018. PMID 30344242 Free PMC article.
The aim of this study was to determine the role of three cell culture media on transcriptional expression of breast cancer markers in three breast cancer reference cell lines (MCF7, SkBr3 and MDA-MB-436). ...Media containing mitogens and higher vitamin content improved efficacy of cell culture in terms of cell yields, although greatly increased growth times....
The aim of this study was to determine the role of three cell culture media on transcriptional expression of breast cancer mar …
Conditioned media from contracting skeletal muscle potentiates insulin secretion and enhances mitochondrial energy metabolism of pancreatic beta-cells.
Barlow J and Solomon TPJ. Metabolism 2019. PMID 30445139
We hypothesised that media conditioned by contracting skeletal muscle will influence insulin secretion and mitochondrial energy metabolism in β-cells under normal and type-2 diabetic conditions. METHODS: INS-1 832/3 pseudoislets were perifused with media from C2C12 myotubes treated with or without electrical pulse stimulation (EPS; 40 V, 1.0 Hz, 2 ms). ...
We hypothesised that media conditioned by contracting skeletal muscle will influence insulin secretion and mitochondrial energy me
Atorvastatin and Conditioned Media from Atorvastatin-Treated Human Hematopoietic Stem/Progenitor-Derived Cells Show Proangiogenic Activity In Vitro but Not In Vivo.
Nowak WN, et al. Mediators Inflamm 2019. PMID 31396016 Free PMC article.
CD34(+) cells after six days in culture were stimulated with atorvastatin (AT), acetylsalicylic acid (ASA), sulforaphane (SR), resveratrol (RV), or metformin (Met) for 48 h. ...Then, we used atorvastatin-stimulated cells or conditioned media from them in the Matrigel plug in vivo angiogenic assay. Neither AT alone in control media nor conditioned media nor AT-stimulated cells affected numbers of endothelial cells in the plug or plug's vascularization. ...
CD34(+) cells after six days in culture were stimulated with atorvastatin (AT), acetylsalicylic acid (ASA), sulforaphane (SR), resver …
Conditioned media from AICAR-treated skeletal muscle cells increases neuronal differentiation of adult neural progenitor cells.
Moon HY, et al. Neuropharmacology 2019. PMID 30391731 Free PMC article.
In rodents, voluntary wheel running increases the production of new neurons and upregulates neurotrophin levels in the hippocampus, as well as improving synaptic plasticity, memory function and mood. ...Proteomic analysis of this fraction identified proteins known to be involved in energy metabolism, cell migration, adhesion and neurogenesis. ...
In rodents, voluntary wheel running increases the production of new neurons and upregulates neurotrophin levels in the hippocampus, as well …
Alzheimer's disease pathology propagation by exosomes containing toxic amyloid-beta oligomers
Sardar Sinha M, et al. Acta Neuropathol 2018. PMID 29934873 Free PMC article.
Recent findings indicate that toxic oligomers of amyloid-beta may cause propagation of pathology in a prion-like manner, although the underlying mechanisms are incompletely understood. Here we show that small extracellular vesicles, exosomes, from Alzheimer patients' brains contain increased levels of amyloid-beta oligomers and can act as vehicles for the neuron-to-neuron transfer of such toxic species in recipient neurons in culture. ...
Recent findings indicate that toxic oligomers of amyloid-beta may cause propagation of pathology in a prion-like manner, although the underl …
Supplementation with IL-6 and Muscle Cell Culture Conditioned Media Enhances Myogenic Differentiation of Adipose Tissue-Derived Stem Cells through STAT3 Activation.
Seo E, et al. Int J Mol Sci 2018. PMID 29882916 Free PMC article.
Mature skeletal muscle cells cannot be expanded in culture systems. Therefore, it is difficult to construct an in vitro model for muscle diseases. To establish an efficient protocol for myogenic differentiation of human adipose tissue-derived stem cells (hADSCs), we investigated whether addition of IL-6 and/or myocyte-conditioned media (CM) to conventional differentiation media can shorten the differentiation period. hADSCs were differentiated to myocytes using the conventional protocol or modified with the addition of 25 pg/mL IL-6 and/or C2C12 CM (25% v/v). ...
Mature skeletal muscle cells cannot be expanded in culture systems. Therefore, it is difficult to construct an in vitro model for mus …
14,482 results
Jump to page
Feedback