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3'-end labeling of RNA with [5'-32P]Cytidine 3',5'-bis(phosphate) and T4 RNA ligase 1.
Nilsen TW. Nilsen TW. Cold Spring Harb Protoc. 2014 Apr 1;2014(4):444-6. doi: 10.1101/pdb.prot080713. Cold Spring Harb Protoc. 2014. PMID: 24692494
This protocol is used to radiolabel the 3' ends of RNAs, either synthesized by in vitro transcription or purified from cells or tissues, by ligation of [5'-(32)P]cytidine 3',5'-bis(phosphate) (pCp). [5'-(32)P]pCp can be obtained commercially or prepared in th …
This protocol is used to radiolabel the 3' ends of RNAs, either synthesized by in vitro transcription or purified from cells or tissu …
Interaction of cytidine 3'-monophosphate and uridine 3'-monophosphate with ribonuclease a at the denaturation temperature.
Schwarz FP. Schwarz FP. Biochemistry. 1988 Nov 1;27(22):8429-36. doi: 10.1021/bi00422a020. Biochemistry. 1988. PMID: 3242592
Differential scanning calorimetry (DSC) measurements were performed on the thermal denaturation of ribonuclease a and ribonuclease a complexed with an inhibitor, cytidine or uridine 3'-monophosphate, in sodium acetate buffered solutions. ...Binding enthalpies …
Differential scanning calorimetry (DSC) measurements were performed on the thermal denaturation of ribonuclease a and ribonuclease a complex …
Metabolomic effects of CeO2, SiO2 and CuO metal oxide nanomaterials on HepG2 cells.
Kitchin KT, Stirdivant S, Robinette BL, Castellon BT, Liang X. Kitchin KT, et al. Part Fibre Toxicol. 2017 Nov 29;14(1):50. doi: 10.1186/s12989-017-0230-4. Part Fibre Toxicol. 2017. PMID: 29187207 Free PMC article.
BACKGROUND: To better assess potential hepatotoxicity of nanomaterials, human liver HepG2 cells were exposed for 3 days to five different CeO(2) (either 30 or 100 mug/ml), 3 SiO(2) based (30 mug/ml) or 1 CuO (3 mug/ml) nanomaterials with dry primary particle …
BACKGROUND: To better assess potential hepatotoxicity of nanomaterials, human liver HepG2 cells were exposed for 3 days to five diffe …
The mechanism of action of ribonuclease.
Roberts GC, Dennis EA, Meadows DH, Cohen JS, Jardetzky O. Roberts GC, et al. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1151-8. doi: 10.1073/pnas.62.4.1151. Proc Natl Acad Sci U S A. 1969. PMID: 5256413 Free PMC article.
In contrast, in the pseudorotation mechanism one histidine residue performs all the catalytic functions, while the other serves only to bind the phosphate anion; this necessarily involves pseudorotation of the intermediate and specific protonation of the leaving group by the enzy …
In contrast, in the pseudorotation mechanism one histidine residue performs all the catalytic functions, while the other serves only to bind …
Measurement of biochemical affinities with a Gill titration calorimeter.
Harrous ME, Parody-Morreale A. Harrous ME, et al. Anal Biochem. 1997 Dec 1;254(1):96-108. doi: 10.1006/abio.1997.2386. Anal Biochem. 1997. PMID: 9398351
The relative precision in the calculated constants ranges from 3 to 80% depending on the macromolecule concentration and kc value in the experiment. These results were checked with the study of the reactions of beta-trypsin with its inhibitor and ribonuclease A with cyt
The relative precision in the calculated constants ranges from 3 to 80% depending on the macromolecule concentration and kc value in …
Mechanism of action of polymeric aurintricarboxylic acid, a potent inhibitor of protein--nucleic acid interactions.
González RG, Haxo RS, Schleich T. González RG, et al. Biochemistry. 1980 Sep 2;19(18):4299-303. doi: 10.1021/bi00559a023. Biochemistry. 1980. PMID: 6158332
The mechanism of inhibition of protein--nucleic acid complex formation by polymeric aurintricarboxylic acid (ATA) was investigated by proton magnetic resonance spectroscopy. ...The epsilon-methylene protons of the lysyl side chains were also broadened upon the bindi …
The mechanism of inhibition of protein--nucleic acid complex formation by polymeric aurintricarboxylic acid (ATA) was investig …
A crystalline end product produced by the hydrolytic cleavage of an RNA-like fragment by an organometallointercalator: 1,10-phenanthroline-platinum(II)-ethylenediamine-cytidine 3' monophosphate.
Vijay-Kumar S, Sakore TD, Sobell HM. Vijay-Kumar S, et al. Nucleic Acids Res. 1984 Apr 25;12(8):3649-57. doi: 10.1093/nar/12.8.3649. Nucleic Acids Res. 1984. PMID: 6203100 Free PMC article.
1,10-Phenanthroline-platinum(II)-ethylenediamine ( PEPt ) forms a crystalline complex with cytidine-3'-phosphate (3'-CMP) and its structure has been determined by X-ray crystallography. 3'-CMP molecules are hemiprotonated and form hydrogen-bonded pairs …
1,10-Phenanthroline-platinum(II)-ethylenediamine ( PEPt ) forms a crystalline complex with cytidine-3'-phosphate (3'-CM …
18 results