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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1959 1
1961 1
1962 4
1963 5
1964 8
1966 4
1969 1
1971 14
1972 211
1973 268
1974 282
1975 231
1976 182
1977 225
1978 191
1979 219
1980 196
1981 209
1982 199
1983 180
1984 234
1985 265
1986 220
1987 216
1988 229
1989 270
1990 302
1991 439
1992 656
1993 777
1994 390
1995 374
1996 391
1997 335
1998 362
1999 350
2000 335
2001 349
2002 331
2003 372
2004 357
2005 400
2006 380
2007 391
2008 397
2009 442
2010 449
2011 465
2012 502
2013 513
2014 507
2015 493
2016 449
2017 468
2018 457
2019 248
2020 3
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16,042 results
Results by year
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Page 1
Robust Genome Editing with Short Single-Stranded and Long, Partially Single-Stranded DNA Donors in Caenorhabditis elegans.
Dokshin GA, et al. Genetics 2018. PMID 30213854 Free PMC article.
CRISPR-based genome editing using ribonucleoprotein complexes and synthetic single-stranded oligodeoxynucleotide (ssODN) donors can be highly effective. ...In addition, we describe a novel PCR-based, partially single-stranded, "hybrid" donor design that yields high efficiency editing with large (kilobase-scale) constructs. ...
CRISPR-based genome editing using ribonucleoprotein complexes and synthetic single-stranded oligodeoxynucleotide (ssODN) donor …
HMCES Maintains Genome Integrity by Shielding Abasic Sites in Single-Strand DNA.
Mohni KN, et al. Cell 2019. PMID 30554877 Free PMC article.
HMCES acts at replication forks, binds PCNA and single-stranded DNA, and generates a DNA-protein crosslink to shield abasic sites from error-prone processing. ...Thus, HMCES is an ancient DNA lesion recognition protein that preserves genome integrity by promoting error-free repair of abasic sites in single-stranded DNA....
HMCES acts at replication forks, binds PCNA and single-stranded DNA, and generates a DNA-protein crosslink to sh …
Quantitative sensing and signalling of single-stranded DNA during the DNA damage response.
Bantele SCS, et al. Nat Commun 2019. PMID 30808869 Free PMC article.
The DNA damage checkpoint senses the presence of DNA lesions and controls the cellular response thereto. A crucial DNA damage signal is single-stranded DNA (ssDNA), which is frequently found at sites of DNA damage and recruits the sensor checkpoint kinase Mec1-Ddc2. However, how this signal - and therefore the cell's DNA damage load - is quantified, is poorly understood. Here, we use genetic manipulation of DNA end resection to induce quantitatively different ssDNA signals at a site-specific double strand break in budding yeast and identify two distinct signalling circuits within the checkpoint. ...
The DNA damage checkpoint senses the presence of DNA lesions and controls the cellular response thereto. A crucial DNA
Single-molecule DREEM imaging reveals DNA wrapping around human mitochondrial single-stranded DNA binding protein.
Kaur P, et al. Nucleic Acids Res 2018. PMID 30256971 Free PMC article.
Mitochondrial single-stranded DNA binding protein (mtSSB) is an essential component of the mtDNA replication machinery. We utilized single-molecule methods to examine the modes by which human mtSSB binds DNA to help define protein interactions at the mtDNA replication fork. ...Selective binding to ssDNA was confirmed by AFM imaging of individual mtSSB tetramers bound to gapped plasmid DNA substrates bearing defined single-stranded regions. ...
Mitochondrial single-stranded DNA binding protein (mtSSB) is an essential component of the mtDNA replication machinery. …
Recycling of single-stranded DNA-binding protein by the bacterial replisome.
Spenkelink LM, et al. Nucleic Acids Res 2019. PMID 30767010 Free PMC article.
Single-stranded DNA-binding proteins (SSBs) support DNA replication by protecting single-stranded DNA from nucleolytic attack, preventing intra-strand pairing events and playing many other regulatory roles within the replisome. ...Not only does it recruit new SSB molecules from solution to coat newly formed single-stranded DNA on the lagging strand, but it also internally recycles SSB from one Okazaki fragment to the next. ...
Single-stranded DNA-binding proteins (SSBs) support DNA replication by protecting single-stranded
Single-Stranded DNA Oligonucleotides Retain Rise Coordinates Characteristic of Double Helices.
Capobianco A, et al. J Phys Chem B 2018. PMID 30070843
The structures of single-stranded DNA oligonucleotides from dimeric to hexameric sequences have been thoroughly investigated. Computations performed at the density functional level of theory including dispersion forces and solvation show that single-stranded helices adopt conformations very close to crystallographic B-DNA, with rise coordinates amounting up to 3.3 Å. ...
The structures of single-stranded DNA oligonucleotides from dimeric to hexameric sequences have been thoroughly investi …
Protonation of Nucleobases in Single- and Double-Stranded DNA.
González-Olvera JC, et al. Chembiochem 2018. PMID 30073767
More complex model oligonucleotides, each containing a centrally located 5-methylcytosine base, were comparatively evaluated in single-stranded and double-stranded form, by UV spectroscopy and high-field NMR. The N(3) protonation of the 5-methylcytosine moiety in the double-stranded case occurred at much lower pH, at which the duplex was already experiencing general dissociation, than in the single-stranded case. ...
More complex model oligonucleotides, each containing a centrally located 5-methylcytosine base, were comparatively evaluated in single
Comparative analysis of single-stranded DNA donors to generate conditional null mouse alleles.
Lanza DG, et al. BMC Biol 2018. PMID 29925370 Free PMC article.
To test the feasibility of using CRISPR/Cas9 gene editing to generate conditional knockout mice for this large-scale resource, we employed Cas9-initiated homology-driven repair (HDR) with short and long single stranded oligodeoxynucleotides (ssODNs and lssDNAs). ...Regardless of the single-stranded donor utilized, it is essential to screen for sequence errors at sites of HDR and random insertion of donor sequences into the genome....
To test the feasibility of using CRISPR/Cas9 gene editing to generate conditional knockout mice for this large-scale resource, we employed C …
A structural and dynamic model for the assembly of Replication Protein A on single-stranded DNA.
Yates LA, et al. Nat Commun 2018. PMID 30575763 Free PMC article.
Replication Protein A (RPA), the major eukaryotic single stranded DNA-binding protein, binds to exposed ssDNA to protect it from nucleases, participates in a myriad of nucleic acid transactions and coordinates the recruitment of other important players. RPA is a heterotrimer and coats long stretches of single-stranded DNA (ssDNA). The precise molecular architecture of the RPA subunits and its DNA binding domains (DBDs) during assembly is poorly understood. ...
Replication Protein A (RPA), the major eukaryotic single stranded DNA-binding protein, binds to exposed ssDNA to protec …
16,042 results
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