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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1966 4
1973 1
1977 5
1978 16
1979 30
1980 22
1981 36
1982 42
1983 41
1984 58
1985 46
1986 43
1987 42
1988 46
1989 45
1990 49
1991 126
1992 164
1993 725
1994 1931
1995 2205
1996 2154
1997 1866
1998 1761
1999 1852
2000 1833
2001 1802
2002 2022
2003 2405
2004 2396
2005 1915
2006 1560
2007 1405
2008 1415
2009 1375
2010 1223
2011 1283
2012 1302
2013 1188
2014 947
2015 857
2016 856
2017 622
2018 457
2019 241
2020 6
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37,969 results
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Page 1
A Method for Single-Stranded Ancient DNA Library Preparation.
Gansauge MT and Meyer M. Methods Mol Biol 2019. PMID 30875046
Genomic library preparation from highly degraded DNA is more efficient when library molecules are prepared separately from the complementary strands of DNA fragments. We describe a protocol in which libraries are constructed from single DNA strands in a three-step procedure: single-stranded ligation of the first adapter with T4 DNA ligase in the presence of a splinter oligonucleotide, copying of the DNA strand with a proofreading polymerase, and blunt-end ligation of the second double-stranded adapter with T4 DNA ligase....
Genomic library preparation from highly degraded DNA is more efficient when library molecules are prepared separately from the comple …
Genetic spell-checking: gene editing using single-stranded DNA oligonucleotides.
Rivera-Torres N and Kmiec EB. Plant Biotechnol J 2016 - Review. PMID 26402400 Free article.
Single-stranded oligonucleotides (ssODNs) can be used to direct the exchange of a single nucleotide or the repair of a single base within the coding region of a gene in a process that is known, generically, as gene editing. ...Double-strand DNA breakage and the activation of the DNA damage response pathway play key roles in determining the frequency with which gene editing activity takes place. ...
Single-stranded oligonucleotides (ssODNs) can be used to direct the exchange of a single nucleotide or the repair of a
Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks
Trapnell C, et al. Nat Protoc 2012. PMID 22383036 Free PMC article.
Recent advances in high-throughput cDNA sequencing (RNA-seq) can reveal new genes and splice variants and quantify expression genome-wide in a single assay. ...
Recent advances in high-throughput cDNA sequencing (RNA-seq) can reveal new genes and splice variants and quantify expression genome-wide in …
Introduction to Single-Cell RNA Sequencing.
Olsen TK and Baryawno N. Curr Protoc Mol Biol 2018. PMID 29851283
Single-cell RNA-seq (scRNA-seq) represents an approach to overcome this problem. By isolating single cells, capturing their transcripts, and generating sequencing libraries in which the transcripts are mapped to individual cells, scRNA-seq allows assessment of fundamental biological properties of cell populations and biological systems at unprecedented resolution. ...
Single-cell RNA-seq (scRNA-seq) represents an approach to overcome this problem. By isolating single cells, capturing their tr
Smart-seq2 for sensitive full-length transcriptome profiling in single cells.
Picelli S, et al. Nat Methods 2013. PMID 24056875
Single-cell gene expression analyses hold promise for characterizing cellular heterogeneity, but current methods compromise on either the coverage, the sensitivity or the throughput. ...
Single-cell gene expression analyses hold promise for characterizing cellular heterogeneity, but current methods compromise on either
Genomoviridae: a new family of widespread single-stranded DNA viruses.
Krupovic M, et al. Arch Virol 2016. PMID 27343045
Here, we introduce a new family of eukaryote-infecting single-stranded (ss) DNA viruses that was created recently by the International Committee on Taxonomy of Viruses (ICTV). The family, named Genomoviridae, contains a single genus, Gemycircularvirus, which currently has one recognized virus species, Sclerotinia gemycircularvirus 1. ...
Here, we introduce a new family of eukaryote-infecting single-stranded (ss) DNA viruses that was created recently by th …
The Future of Cross-Linking and Immunoprecipitation (CLIP)
Ule J, et al. Cold Spring Harb Perspect Biol 2018 - Review. PMID 30068528 Free PMC article.
Cross-linking and immunoprecipitation (CLIP) serves this purpose by exploiting covalent protein-RNA cross-linking and RNA fragmentation, along with a series of stringent purification and quality control steps to prepare complementary DNA (cDNA) libraries for sequencing. ...
Cross-linking and immunoprecipitation (CLIP) serves this purpose by exploiting covalent protein-RNA cross-linking and RNA fragmentation, alo …
Sub-Ensemble Monitoring of DNA Strand Displacement Using Multiparameter Single-Molecule FRET.
Baltierra-Jasso LE, et al. Chemphyschem 2018. PMID 29316151
We monitored the strand displacement from double-stranded DNA (dsDNA) by single-stranded DNA (ssDNA) at 37 °C; the data were modelled as a second-order reaction approaching equilibrium, with a rate constant of 10 m(-1)  s(-1) . ...The single-molecule approach demonstrated here will be useful for studying complex DNA networks....
We monitored the strand displacement from double-stranded DNA (dsDNA) by single-stranded DNA (ssDNA) at 3 …
Furan-PNA: a mildly inducible irreversible interstrand crosslinking system targeting single and double stranded DNA.
Manicardi A, et al. Chem Commun (Camb) 2016. PMID 27147261
We here report on the design and synthesis of tailor-made furan-modified peptide nucleic acid (PNA) probes for covalent targeting of single stranded DNA through a crosslinking strategy. ...The structure of the crosslinked products is characterized and preliminary investigations concerning the application of these systems to double stranded DNA are shown....
We here report on the design and synthesis of tailor-made furan-modified peptide nucleic acid (PNA) probes for covalent targeting of sing
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