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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1946 2
1949 3
1950 1
1952 1
1953 2
1955 2
1957 1
1958 1
1959 4
1960 2
1961 2
1962 3
1963 4
1964 1
1965 2
1983 1
1988 2
1992 28
1993 1684
1994 5526
1995 6645
1996 6318
1997 3735
1998 4619
1999 5306
2000 5474
2001 4903
2002 4762
2003 5469
2004 6073
2005 6043
2006 4956
2007 5269
2008 5160
2009 4581
2010 4736
2011 4562
2012 4584
2013 3983
2014 3442
2015 1664
2016 693
2017 633
2018 557
2019 271
2020 9
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104,357 results
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Page 1
Design of primers and probes for quantitative real-time PCR methods.
Rodríguez A, et al. Methods Mol Biol 2015. PMID 25697650
Design of primers and probes is one of the most crucial factors affecting the success and quality of quantitative real-time PCR (qPCR) analyses, since an accurate and reliable quantification depends on using efficient primers and probes. Design of primers and probes should meet several criteria to find potential primers and probes for specific qPCR assays. The formation of primer-dimers and other non-specific products should be avoided or reduced. ...
Design of primers and probes is one of the most crucial factors affecting the success and quality of quantitative real-time PCR (qPCR …
Site-directed mutagenesis
Bachman J. Methods Enzymol 2013. PMID 24011050
Proximity Ligation Assay (PLA)
Alam MS. Curr Protoc Immunol 2018. PMID 30238640 Free PMC article.
It exploits specific antibodies identifying (either directly or indirectly) the two proteins of interest and utilizes specific DNA primers covalently linked to the antibodies. A hybridization step followed by DNA amplification with fluorescent probes permit visualization of spots of proximity by fluorescence microscopy. ...
It exploits specific antibodies identifying (either directly or indirectly) the two proteins of interest and utilizes specific DNA
Touchdown Polymerase Chain Reaction (PCR)
Green MR and Sambrook J. Cold Spring Harb Protoc 2018. PMID 29717053
In touchdown PCR the temperature selected for the annealing step is initially set 5°C-10°C higher than the calculated T(m) of the primers. ...The use of touchdown PCR is essential when the sequence of the primer might not match that of the target-for example, if the sequence of the primer has been deduced from amino acid sequences, when the template DNA may contain several closely related targets, or when the target DNA is of a different species from that used to design the primers....
In touchdown PCR the temperature selected for the annealing step is initially set 5°C-10°C higher than the calculated T(m) of the primers
Specific primer design for the polymerase chain reaction
Chuang LY, et al. Biotechnol Lett 2013 - Review. PMID 23794048
The design of primers has a major impact on the success of PCR in relation to the specificity and yield of the amplified product. ...
The design of primers has a major impact on the success of PCR in relation to the specificity and yield of the amplified product. ...
Rapid and simple method of qPCR primer design
Thornton B and Basu C. Methods Mol Biol 2015. PMID 25697660
In qPCR, a reporter dye system is used which intercalates with DNA's region of interest and detects DNA amplification. Some of the popular reporter systems used in qPCR are the following: Molecular Beacon(®), SYBR Green(®), and Taqman(®). ...Here we have shown how to use some freely available web-based software programs (such as Primerquest(®), Unafold(®), and Beacon designer(®)) to design qPCR primers....
In qPCR, a reporter dye system is used which intercalates with DNA's region of interest and detects DNA amplification. Some of …
Primer design for RT-PCR
Li K and Brownley A. Methods Mol Biol 2010. PMID 20301004
Properly designed primers will increase PCR amplification efficiency as well as isolate the targeted sequence of interest with higher specificity. ...
Properly designed primers will increase PCR amplification efficiency as well as isolate the targeted sequence of interest with higher …
In silico PCR primer designing and validation
Kumar A and Chordia N. Methods Mol Biol 2015. PMID 25697657
Polymerase chain reaction (PCR) is an enzymatic reaction whose efficiency and sensitivity largely depend on the efficiency of the primers that are used for the amplification of a concerned gene/DNA fragment. ...Thereafter, the primers designed in silico can be validated in the wet lab. After that, these validated primers can be synthesized for use in the amplification of concerned gene/DNA fragment....
Polymerase chain reaction (PCR) is an enzymatic reaction whose efficiency and sensitivity largely depend on the efficiency of the primers
Enhancement of PCR Sensitivity and Yield Using Thiol-modified Primers.
Bai Y, et al. Sci Rep 2018. PMID 30291287 Free PMC article.
Amplification with thiol-modified primers was strongly inhibited by the presence of extraneous proteins relative to standard DNA primers, which indicates that thiol-modified primers may be inhibited due to interaction with these proteins. In contaminant-free reactions, however, the thiol-modified primers might interact more strongly with DNA polymerase, which could in turn improve PCR amplification....
Amplification with thiol-modified primers was strongly inhibited by the presence of extraneous proteins relative to standard DNA
MethPrimer: designing primers for methylation PCRs.
Li LC and Dahiya R. Bioinformatics 2002. PMID 12424112
RESULTS: MethPrimer, based on Primer 3, is a program for designing PCR primers for methylation mapping. It first takes a DNA sequence as its input and searches the sequence for potential CpG islands. Primers are then picked around the predicted CpG islands or around regions specified by users. MethPrimer can design primers for BSP and MSP. ...
RESULTS: MethPrimer, based on Primer 3, is a program for designing PCR primers for methylation mapping. It first takes a DNA s …
104,357 results
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