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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1946 2
1949 3
1950 1
1952 1
1953 2
1955 2
1957 1
1958 1
1959 4
1960 2
1961 2
1962 3
1963 4
1964 1
1965 2
1983 1
1988 2
1992 28
1993 1684
1994 5526
1995 6645
1996 6318
1997 3735
1998 4619
1999 5306
2000 5474
2001 4903
2002 4762
2003 5469
2004 6073
2005 6043
2006 4956
2007 5269
2008 5160
2009 4581
2010 4736
2011 4562
2012 4584
2013 3983
2014 3442
2015 1664
2016 693
2017 633
2018 557
2019 280
2020 11
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104,367 results
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Page 1
Proximity Ligation Assay (PLA)
Alam MS. Curr Protoc Immunol 2018. PMID 30238640 Free PMC article.
It exploits specific antibodies identifying (either directly or indirectly) the two proteins of interest and utilizes specific DNA primers covalently linked to the antibodies. A hybridization step followed by DNA amplification with fluorescent probes permit visualization of spots of proximity by fluorescence microscopy. ...
It exploits specific antibodies identifying (either directly or indirectly) the two proteins of interest and utilizes specific DNA
ChIP-Quantitative Polymerase Chain Reaction (ChIP-qPCR)
Kim TH and Dekker J. Cold Spring Harb Protoc 2018. PMID 29717044
It is critical to determine if the ChIP actually enriched the DNA sequences that are associated with the target protein. If there are known genomic binding sites, primers can be designed for quantitative PCR (qPCR) to determine if the known sites are specifically enriched by immunoprecipitation. If there are no known sites but candidate target genes are available, one can consider designing qPCR primers along the length of potential regulatory regions such as promoters and conserved noncoding sequences within intergenic and genic regions. ...
It is critical to determine if the ChIP actually enriched the DNA sequences that are associated with the target protein. If there are …
Human DNA polymerase η has reverse transcriptase activity in cellular environments.
Su Y, et al. J Biol Chem 2019. PMID 30842261 Free PMC article.
Here, we show that purified hpol η adds rNTPs to DNA primers at physiological rNTP concentrations and in the presence of competing dNTPs. ...XP-V cell extracts did not add dNTPs to DNA primers hybridized to RNA, but could when hpol η was expressed in the cells. HEK293T cell extracts could add dNTPs to DNA primers hybridized to RNA, but lost this ability if hpol η was deleted. ...
Here, we show that purified hpol η adds rNTPs to DNA primers at physiological rNTP concentrations and in the presence of compe …
Using CRISPR/Cas9 System to Introduce Targeted Mutation in Arabidopsis
Lee ZH, et al. Methods Mol Biol 2018. PMID 30043366
Our protocol contains five different steps: (1) adaptor primers design, (2) cloning of sgRNA expression cassettes, (3) Golden Gate cloning, (4) E. coli and Agrobacterium transformation, and (5) Arabidopsis transformation....
Our protocol contains five different steps: (1) adaptor primers design, (2) cloning of sgRNA expression cassettes, (3) Golden Gate cl …
New universal ITS2 primers for high-resolution herbivory analyses using DNA metabarcoding in both tropical and temperate zones.
Moorhouse-Gann RJ, et al. Sci Rep 2018. PMID 29867115 Free PMC article.
In vitro, the primers amplified 99% of Mauritian (n = 169) and 100% of UK (n = 33) species, and co-amplified multiple plant species from degraded faecal DNA from reptiles and birds in two case studies. ...With short amplicons of 187-387 bp, these primers are suitable for metabarcoding plant DNA from faecal samples, across a broad geographic range, whilst delivering unparalleled taxonomic resolution....
In vitro, the primers amplified 99% of Mauritian (n = 169) and 100% of UK (n = 33) species, and co-amplified multiple plant species f …
Single Core Genome Sequencing for Detection of both Borrelia burgdorferi Sensu Lato and Relapsing Fever Borrelia Species
Lee SH, et al. Int J Environ Res Public Health 2019. PMID 31137527 Free PMC article.
After a diligent search of the GenBank database, we identified two highly conserved segments of DNA sequence among the borrelial 16S rRNA genes. ...This study presented examples of base-calling DNA sequencing electropherograms routinely generated in a clinical diagnostic laboratory on DNA extracts of human blood specimens and ticks collected from human skin bites and from the environment. ...
After a diligent search of the GenBank database, we identified two highly conserved segments of DNA sequence among the borrelial 16S …
SHERLOCK: nucleic acid detection with CRISPR nucleases
Kellner MJ, et al. Nat Protoc 2019. PMID 31548639 Free PMC article.
We have recently established a CRISPR-based diagnostic platform that combines nucleic acid pre-amplification with CRISPR-Cas enzymology for specific recognition of desired DNA or RNA sequences. ...We also include guidelines for designing efficient CRISPR RNA (crRNA) and isothermal amplification primers, as well as discuss important considerations for multiplex and quantitative SHERLOCK detection assays....
We have recently established a CRISPR-based diagnostic platform that combines nucleic acid pre-amplification with CRISPR-Cas enzymology for …
Multiple sealed primers-mediated rolling circle amplification strategy for sensitive and specific detection of DNA methyltransferase activity.
Xu X, et al. Talanta 2019. PMID 30609532
The DNA probe has a folded, double-loop structure that seals multiple primers. First, in the presence of DNA MTase, the DNA probe is methylated, which then gets cleaved by the restriction endonuclease and breaks into multiple DNA oligonucleotide fragments. ...When DNA MTase is absent or inactive, the DNA probe is stable and cannot release the primers for RCA reaction. In the proposed strategy, the action of DNA MTase on one DNA probe is converted to the multiple amplifications triggered by multiple released primers. ...
The DNA probe has a folded, double-loop structure that seals multiple primers. First, in the presence of DNA MTase, the …
Activity and fidelity of human DNA polymerase α depend on primer structure.
Baranovskiy AG, et al. J Biol Chem 2018. PMID 29555682 Free PMC article.
DNA polymerase α (Polα) plays an important role in genome replication. In a complex with primase, Polα synthesizes chimeric RNA-DNA primers necessary for replication of both chromosomal DNA strands. ...Moreover, human Polα extended DNA primers with higher efficiency but lower processivity than it did with RNA and chimeric primers. ...
DNA polymerase α (Polα) plays an important role in genome replication. In a complex with primase, Polα synthesizes chimeric RNA-DN
Enhancement of PCR Sensitivity and Yield Using Thiol-modified Primers.
Bai Y, et al. Sci Rep 2018. PMID 30291287 Free PMC article.
Amplification with thiol-modified primers was strongly inhibited by the presence of extraneous proteins relative to standard DNA primers, which indicates that thiol-modified primers may be inhibited due to interaction with these proteins. In contaminant-free reactions, however, the thiol-modified primers might interact more strongly with DNA polymerase, which could in turn improve PCR amplification....
Amplification with thiol-modified primers was strongly inhibited by the presence of extraneous proteins relative to standard DNA
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