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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1976 2
1977 8
1978 24
1979 20
1980 16
1981 17
1982 14
1983 31
1984 23
1985 46
1986 67
1987 61
1988 62
1989 67
1990 126
1991 170
1992 242
1993 201
1994 219
1995 231
1996 248
1997 259
1998 239
1999 251
2000 253
2001 269
2002 287
2003 276
2004 282
2005 316
2006 379
2007 414
2008 406
2009 393
2010 421
2011 402
2012 383
2013 368
2014 351
2015 338
2016 350
2017 367
2018 323
2019 158
2020 3
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8,702 results
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Page 1
Enzyme-Linked Immunosorbent Assays.
Hornbeck PV. Curr Protoc Immunol 2015. PMID 26237010
In all protocols, the solid-phase reagents are incubated with secondary or tertiary reactants covalently coupled to an enzyme. ...As the substrate is hydrolyzed by the bound enzyme conjugate, a colored or fluorescent product is generated. Finally, the product is detected visually or with a microtiter plate reader. ...
In all protocols, the solid-phase reagents are incubated with secondary or tertiary reactants covalently coupled to an enzyme. ...As …
Diagnosis of Zika Virus Infection by Peptide Array and Enzyme-Linked Immunosorbent Assay.
Mishra N, et al. mBio 2018. PMID 29511073 Free PMC article.
Serological analysis enabled discovery of a ZIKV NS2B 20-residue peptide that had high sensitivity (96.0%) and specificity (95.9%) versus natural infection with or vaccination against dengue, chikungunya, yellow fever, West Nile, tick-borne encephalitis, or Japanese encephalitis virus in a microarray assay and an enzyme-linked immunosorbent assay (ELISA) of early-convalescent-phase sera (2 to 3 weeks after onset of symptomatic infection).IMPORTANCE The emergence of Zika virus (ZIKV) as a teratogen is a profound challenge to global public health. ...
Serological analysis enabled discovery of a ZIKV NS2B 20-residue peptide that had high sensitivity (96.0%) and specificity (95.9%) versus na …
Enzyme-Linked Immunosorbent Assay to Quantify Targeting Molecules on Nanoparticles.
Riley RS, et al. Methods Mol Biol 2018. PMID 30051430
Here, we describe methods to conjugate antibodies to silica-gold nanoshells and to quantify the resulting antibody content on the nanoparticles using a solution-based enzyme-linked immunosorbent assay (ELISA). ...
Here, we describe methods to conjugate antibodies to silica-gold nanoshells and to quantify the resulting antibody content on the nan …
Enzyme-linked immunosorbent assays.
Nilsson B. Curr Opin Immunol 1989 - Review. PMID 2486571
A Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assay for Determination of Homoharringtonine.
Pongkitwitoon B, et al. Planta Med 2018. PMID 29490384
The developed indirect competitive enzyme-linked immunosorbent assay (icELISA) using MAb 6A1 was found to be highly specific and sensitive with a limit of detection for HHT of 48.8 ng/mL. Validation assays to evaluate precision and accuracy of the method were conducted by the use of intra- and inter-assay analysis, recovery test, and comparison analysis between the amounts of HHT determined by ELISA and high-performance liquid chromatography. ...
The developed indirect competitive enzyme-linked immunosorbent assay (icELISA) using MAb 6A1 was found to be hig …
Immunometric Double-Antibody Sandwich Enzyme-Linked Immunosorbent Assay.
Kohl TO and Ascoli CA. Cold Spring Harb Protoc 2017. PMID 28572188
The double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) is preferentially used to determine the concentration of unknown antibody in a sample. Pure antigen is not required in this assay; however, the use of a reporter-labeled detection antibody is essential. The double-antibody sandwich ELISA is suitable for epitope mapping of different monoclonal antibodies that have been generated against a single antigen. ...
The double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) is preferentially used to determine the c …
ELISPOT Techniques
Ji N and Forsthuber TG. Methods Mol Biol 2016. PMID 25117217
The enzyme-linked immunospot (ELISPOT) assay is a widely used method for enumerating antigen-specific cytokine-producing or antibody-secreting immune cells. ...Detection and quantification of specific cytokine-producing cells by the ELISPOT assay is based on the formation of visible spots at the site of cytokine release by the cells under investigation (e.g., T cells) using pairs of different capture and detection antibodies under optimized conditions.Here we focus mainly on practical, optimized protocols for cytokine ELISPOT assays for detection of mouse and human cytokine-producing immune cells (e.g., peripheral blood mononuclear cells, PBMC), including suggestions for trouble-shooting and optimizing steps for problematic tissue samples....
The enzyme-linked immunospot (ELISPOT) assay is a widely used method for enumerating antigen-specific cytokine-producin …
An enzyme-linked immunosorbent assay for detection of pyrene and related polycyclic aromatic hydrocarbons.
Meng XY, et al. Anal Biochem 2015. PMID 25524617
A competitive enzyme-linked immunosorbent assay (ELISA) was developed for detection of pyrene and some homologues in water samples. The detection limit of the assay was 65.08 pg ml(-1). The average recoveries of PAHs from tap water, lake water, and mineral water were 99.13, 99.74, and 99.19%, respectively, indicating that matrices of water samples do not interfere with the assay. ...
A competitive enzyme-linked immunosorbent assay (ELISA) was developed for detection of pyrene and some homologue …
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