Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation

Search Page

Filters

My Custom Filters

Results by year

Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1781 37
1782 18
1783 22
1784 27
1785 43
1786 60
1787 43
1788 33
1789 32
1790 44
1791 19
1792 33
1793 17
1794 18
1795 13
1796 14
1797 59
1798 15
1799 291
1800 377
1801 360
1802 283
1803 269
1804 289
1805 314
1806 282
1807 295
1808 289
1809 265
1810 267
1811 322
1812 238
1813 265
1814 285
1815 222
1816 315
1817 288
1818 303
1819 225
1820 213
1821 270
1822 247
1823 291
1824 269
1825 262
1826 385
1827 326
1828 467
1829 645
1830 569
1831 627
1832 313
1833 293
1834 287
1835 305
1836 271
1837 350
1838 484
1839 546
1840 695
1841 1154
1842 1488
1843 1118
1844 1248
1845 1277
1846 1406
1847 1367
1848 1203
1849 1207
1850 1126
1851 1228
1852 1310
1853 1558
1854 1306
1855 1586
1856 1587
1857 1488
1858 1542
1859 1562
1860 1632
1861 1275
1862 1112
1863 995
1864 1138
1865 1151
1866 1477
1867 1723
1868 1813
1869 1909
1870 1809
1871 2238
1872 2242
1873 2313
1874 2508
1875 2402
1876 2095
1877 2179
1878 2454
1879 2399
1880 2646
1881 3149
1882 2742
1883 3126
1884 3049
1885 3337
1886 3291
1887 3965
1888 4002
1889 4194
1890 4024
1891 3607
1892 4080
1893 4370
1894 3801
1895 4129
1896 4412
1897 4689
1898 4631
1899 4169
1900 3785
1901 3886
1902 3949
1903 3885
1904 4056
1905 4067
1906 3872
1907 4323
1908 4459
1909 4687
1910 4799
1911 5006
1912 4906
1913 5105
1914 5135
1915 4640
1916 4543
1917 4281
1918 4567
1919 4619
1920 4960
1921 4628
1922 4123
1923 4432
1924 4851
1925 4990
1926 5342
1927 5887
1928 5479
1929 5741
1930 5630
1931 5496
1932 5548
1933 5567
1934 5506
1935 5661
1936 5384
1937 5308
1938 5765
1939 5541
1940 4998
1941 4838
1942 4775
1943 4517
1944 4745
1945 20421
1946 53739
1947 64968
1948 70726
1949 62805
1950 85750
1951 103810
1952 108147
1953 108914
1954 105913
1955 108541
1956 107005
1957 111515
1958 109458
1959 109989
1960 112211
1961 120166
1962 125670
1963 141520
1964 162087
1965 176641
1966 179753
1967 191498
1968 207843
1969 215070
1970 218975
1971 222866
1972 227505
1973 230628
1974 234508
1975 248222
1976 250193
1977 249431
1978 257754
1979 265014
1980 266763
1981 271107
1982 282639
1983 294212
1984 302097
1985 318314
1986 330746
1987 346619
1988 362875
1989 376807
1990 381792
1991 379432
1992 380853
1993 388363
1994 399984
1995 409063
1996 418837
1997 416405
1998 433826
1999 450872
2000 487225
2001 499636
2002 517528
2003 545097
2004 586957
2005 645911
2006 690589
2007 728316
2008 775656
2009 815310
2010 877946
2011 954523
2012 1020414
2013 1071480
2014 1125478
2015 1167641
2016 1202089
2017 1226066
2018 1269858
2019 1330712
2020 1526666
2021 1671700
2022 1673077
2023 1586623
2024 1627952
2025 704111
2026 73
2027 1

Publication date

Text availability

Article attribute

Article type

Additional filters

Article Language

Species

Sex

Age

Other

Search Results

36,441,713 results

Results by year

Filters applied: . Clear all
Page 1
Consensus protein design.
Porebski BT, Buckle AM. Porebski BT, et al. Protein Eng Des Sel. 2016 Jul;29(7):245-51. doi: 10.1093/protein/gzw015. Epub 2016 Jun 5. Protein Eng Des Sel. 2016. PMID: 27274091 Free PMC article. Review.
A popular and successful strategy in semi-rational design of protein stability is the use of evolutionary information encapsulated in homologous protein sequences. ...
A popular and successful strategy in semi-rational design of protein stability is the use of evolutionary information encapsul …
Thermodynamics of globular protein native structure.
Khechinashvili NN, Kondratyev MS, Polozov RV. Khechinashvili NN, et al. J Biomol Struct Dyn. 2023 May;41(8):3218-3221. doi: 10.1080/07391102.2022.2046637. Epub 2022 Mar 29. J Biomol Struct Dyn. 2023. PMID: 35345984
The temperature dependence of the partial heat capacity of the native protein structure in an aqueous solution has been analyzed. It is shown that the strictly linear temperature dependence is due to the contributions of the vibrational and conformatio …
The temperature dependence of the partial heat capacity of the native protein structure in an aqueous solution has been analyz …
Macromolecular Crowding Is More than Hard-Core Repulsions.
Speer SL, Stewart CJ, Sapir L, Harries D, Pielak GJ. Speer SL, et al. Annu Rev Biophys. 2022 May 9;51:267-300. doi: 10.1146/annurev-biophys-091321-071829. Epub 2022 Mar 3. Annu Rev Biophys. 2022. PMID: 35239418 Review.
We review the experimental evidence that crowding affects the equilibrium thermodynamics of protein stability and protein association and discuss the theories employed to explain these observations. ...However, experiment-based efforts conducted under physiol …
We review the experimental evidence that crowding affects the equilibrium thermodynamics of protein stability and prote …
Cold denaturation as a tool to measure protein stability.
Sanfelice D, Temussi PA. Sanfelice D, et al. Biophys Chem. 2016 Jan;208:4-8. doi: 10.1016/j.bpc.2015.05.007. Epub 2015 May 22. Biophys Chem. 2016. PMID: 26026885 Free PMC article. Review.
Protein stability is an important issue for the interpretation of a wide variety of biological problems but its assessment is at times difficult. ...The calculation of this curve is greatly facilitated whenever it is possible to observe cold denaturation …
Protein stability is an important issue for the interpretation of a wide variety of biological problems but its assessment is …
Protein StabilityAnalysis of Heat and Cold Denaturation without and with Unfolding Models.
Seelig J, Seelig A. Seelig J, et al. J Phys Chem B. 2023 Apr 20;127(15):3352-3363. doi: 10.1021/acs.jpcb.3c00882. Epub 2023 Apr 11. J Phys Chem B. 2023. PMID: 37040567 Free PMC article.
Protein stability is important in many areas of life sciences. Thermal protein unfolding is investigated extensively with various spectroscopic techniques. ...This is illustrated with experimental examples for heat and cold denaturation of lysozyme and …
Protein stability is important in many areas of life sciences. Thermal protein unfolding is investigated extensively with vari …
Time-Dependent Protein Thermostability Assay.
Vandecaetsbeek I, Vangheluwe P. Vandecaetsbeek I, et al. Methods Mol Biol. 2016;1377:79-85. doi: 10.1007/978-1-4939-3179-8_9. Methods Mol Biol. 2016. PMID: 26695024
Membrane protein purification often yields rather unstable proteins impeding functional and structural protein characterization. Low protein stability also leads to low purification yields as a result of protein degradation, aggregation, precipitation, and folding i …
Membrane protein purification often yields rather unstable proteins impeding functional and structural protein characterization. Low prot …
Protein Mutations and Stability, a Link with Disease: The Case Study of Frataxin.
Puglisi R. Puglisi R. Biomedicines. 2022 Feb 11;10(2):425. doi: 10.3390/biomedicines10020425. Biomedicines. 2022. PMID: 35203634 Free PMC article. Review.
In this review, we explore the paradigmatic example of frataxin, an iron binding protein involved in Fe-S cluster biogenesis, and whose impairment causes a neurodegenerative disease called Friedreich's Ataxia (FRDA). We summarize what is known about most common point mutat …
In this review, we explore the paradigmatic example of frataxin, an iron binding protein involved in Fe-S cluster biogenesis, and who …
Recipes for Inducing Cold Denaturation in an Otherwise Stable Protein.
Bitonti A, Puglisi R, Meli M, Martin SR, Colombo G, Temussi PA, Pastore A. Bitonti A, et al. J Am Chem Soc. 2022 Apr 27;144(16):7198-7207. doi: 10.1021/jacs.1c13355. Epub 2022 Apr 15. J Am Chem Soc. 2022. PMID: 35427450 Free PMC article.
A way to approach them is to be able to induce cold denaturation in an otherwise stable protein by means of mutations. Here, we studied CyaY, a relatively stable bacterial protein with no detectable cold denaturation and a high melting temperature of 5 …
A way to approach them is to be able to induce cold denaturation in an otherwise stable protein by means of mutations. Here, w …
Rational-Design Engineering to Improve Enzyme Thermostability.
Pongsupasa V, Anuwan P, Maenpuen S, Wongnate T. Pongsupasa V, et al. Methods Mol Biol. 2022;2397:159-178. doi: 10.1007/978-1-0716-1826-4_9. Methods Mol Biol. 2022. PMID: 34813064
The fundamentals of thermostability engineering need to be carried out for proteins with low thermal stability to expand their utilization. Thus, comprehension of the thermal stability regulating factors of proteins is needful for the engineering of their thermostabilit …
The fundamentals of thermostability engineering need to be carried out for proteins with low thermal stability to expand their utiliz …
Heat and cold denaturation of yeast frataxin: The effect of pressure.
Puglisi R, Cioni P, Gabellieri E, Presciuttini G, Pastore A, Temussi PA. Puglisi R, et al. Biophys J. 2022 Apr 19;121(8):1502-1511. doi: 10.1016/j.bpj.2022.03.010. Epub 2022 Mar 9. Biophys J. 2022. PMID: 35278425 Free PMC article.
Yfh1 is a yeast protein with the peculiar characteristic to undergo, in the absence of salt, cold denaturation at temperatures above the water freezing point. This feature makes the protein particularly interesting for studies aiming at understanding the rules that …
Yfh1 is a yeast protein with the peculiar characteristic to undergo, in the absence of salt, cold denaturation at temperatures …
36,441,713 results
You have reached the last available page of results. Please see the User Guide for more information.