Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation

Search Page

MyNCBI Filters
Results by year

Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1999 1
2000 1
2001 9
2002 1
2003 14
2004 531
2005 1433
2006 1457
2007 1711
2008 1762
2009 2012
2010 2010
2011 1897
2012 1990
2013 1821
2014 1579
2015 1475
2016 1288
2017 1104
2018 943
2019 301
2020 6
Text availability
Article attribute
Article type
Publication date

Search Results

20,744 results
Results by year
Filters applied: . Clear all
Page 1
Fluorescent proteins and their applications in imaging living cells and tissues.
Chudakov DM, et al. Physiol Rev 2010 - Review. PMID 20664080 Free article.
Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its homologs from diverse marine animals are widely used as universal genetically encoded fluorescent labels. ...Fast-maturing fluorescent proteins, cell clocks, and timers further expand the options for real time studies in living tissues. ...
Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its homologs from diverse marine animals are widely u
Green fluorescent protein: structure, folding and chromophore maturation.
Craggs TD. Chem Soc Rev 2009 - Review. PMID 19771333
The prolific use of green fluorescent protein and its variants throughout cellular biology relies on the post-translational formation of the chromophore, which proceeds without the need for any additional enzymes or cofactors, except molecular oxygen. ...A greater understanding of the maturation process of fluorescent proteins from both jellyfish and coral species will profit the ongoing quest for brighter, faster maturing, genetically-encodable fluorescent probes of all colours, thus increasing their utility throughout the biomedical sciences....
The prolific use of green fluorescent protein and its variants throughout cellular biology relies on the post-translational fo …
Two-photon directed evolution of green fluorescent proteins.
Stoltzfus CR, et al. Sci Rep 2015. PMID 26145791 Free PMC article.
Directed evolution has been used extensively to improve the properties of a variety of fluorescent proteins (FPs). Evolutionary strategies, however, have not yet been used to improve the two-photon absorption (2PA) properties of a fluorescent protein, properties that are important for two-photon imaging in living tissues, including the brain. Here we demonstrate a technique for quantitatively screening the two-photon excited fluorescence (2PEF) efficiency and 2PA cross section of tens of thousands of mutant FPs expressed in E. coli colonies. ...
Directed evolution has been used extensively to improve the properties of a variety of fluorescent proteins (FPs). Evolutionar …
Development and Applications of Superfolder and Split Fluorescent Protein Detection Systems in Biology.
Pedelacq JD and Cabantous S. Int J Mol Sci 2019 - Review. PMID 31311175 Free PMC article.
Molecular engineering of the green fluorescent protein (GFP) into a robust and stable variant named Superfolder GFP (sfGFP) has revolutionized the field of biosensor development and the use of fluorescent markers in diverse area of biology. sfGFP-based self-associating bipartite split-FP systems have been widely exploited to monitor soluble expression in vitro, localization, and trafficking of proteins in cellulo. ...
Molecular engineering of the green fluorescent protein (GFP) into a robust and stable variant named Superfolder GFP (sfGFP) ha …
Molecular Dynamic Indicators of the Photoswitching Properties of Green Fluorescent Proteins.
Smyrnova D, et al. J Phys Chem B 2015. PMID 26305506
Reversibly photoswitchable fluorescent proteins (RSFPs) are highly useful probes for a range of applications including diffraction-unlimited fluorescence microscopy. ...Overall, we show that PLS-FMA yields information, complementary to static structures, that can guide the rational design of fluorescent proteins....
Reversibly photoswitchable fluorescent proteins (RSFPs) are highly useful probes for a range of applications including diffrac …
The evolution of genes encoding for green fluorescent proteins: insights from cephalochordates (amphioxus).
Yue JX, et al. Sci Rep 2016. PMID 27311567 Free PMC article.
Green Fluorescent Protein (GFP) was originally found in cnidarians, and later in copepods and cephalochordates (amphioxus) (Branchiostoma spp). ...Dim fluorescence was found throughout the body in adults of Asymmetron lucayanum, and, as in Branchiostoma floridae, was especially intense in the ripe ovaries. ...
Green Fluorescent Protein (GFP) was originally found in cnidarians, and later in copepods and cephalochordates (amphioxus) (Br
Non-fluorescent mutant of green fluorescent protein sheds light on the mechanism of chromophore formation.
Bartkiewicz M, et al. FEBS Lett 2018. PMID 29637558 Free article.
The mechanism of green fluorescent protein (GFP) chromophore formation is still not clearly defined. Two mechanisms have been proposed: cyclisation-dehydration-oxidation (Mechanism A) and cyclisation-oxidation-dehydration (Mechanism B). To distinguish between these mechanisms, we generated a non-fluorescent mutant of GFP, S65T/G67A-GFP. This mutant folds to a stable, native-like structure but lacks fluorescence due to interruption of the chromophore maturation process. ...
The mechanism of green fluorescent protein (GFP) chromophore formation is still not clearly defined. Two mechanisms have been …
CRISPR/Cas9 in the Chicken Embryo
Morin V, et al. Methods Mol Biol 2017. PMID 28809017
20,744 results
Jump to page
Feedback