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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1999 1
2000 1
2001 6
2002 2
2003 16
2004 797
2005 1899
2006 1585
2007 1875
2008 2008
2009 1820
2010 1869
2011 1989
2012 2145
2013 2052
2014 1849
2015 1732
2016 1451
2017 1293
2018 1043
2019 319
2020 3
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22,941 results
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Page 1
Versatile protein tagging in cells with split fluorescent protein.
Kamiyama D, et al. Nat Commun 2016. PMID 26988139 Free PMC article.
In addition to the popular method of fluorescent protein fusion, live cell protein imaging has now seen more and more application of epitope tags. ...To address their background issue, we adapt self-complementing split fluorescent proteins as epitope tags for live cell protein labelling. ...
In addition to the popular method of fluorescent protein fusion, live cell protein imaging has now seen more and more application of …
Comparative assessment of fluorescent proteins for in vivo imaging in an animal model system.
Heppert JK, et al. Mol Biol Cell 2016. PMID 27385332 Free PMC article.
To address the gap, we quantitatively assessed fluorescent protein properties in vivo in an animal model system. We generated transgenic Caenorhabditis elegans strains expressing green, yellow, or red fluorescent proteins in embryos and imaged embryos expressing different fluorescent proteins under the same conditions for direct comparison. ...These results identify ideal fluorescent proteins for imaging in vivo in C. elegans embryos and suggest good candidate fluorescent proteins to test in other animal model systems for in vivo imaging experiments....
To address the gap, we quantitatively assessed fluorescent protein properties in vivo in an animal model system. We generated transge …
Systematic characterization of maturation time of fluorescent proteins in living cells.
Balleza E, et al. Nat Methods 2018. PMID 29320486 Free PMC article.
The slow maturation time of fluorescent proteins (FPs) limits the temporal accuracy of measurements of rapid processes such as gene expression dynamics and effectively reduces fluorescence signal in growing cells. ...
The slow maturation time of fluorescent proteins (FPs) limits the temporal accuracy of measurements of rapid processes such as …
Fluorescence live cell imaging.
Ettinger A and Wittmann T. Methods Cell Biol 2014 - Review. PMID 24974023 Free PMC article.
Fluorescence microscopy of live cells has become an integral part of modern cell biology. Fluorescent protein (FP) tags, live cell dyes, and other methods to fluorescently label proteins of interest provide a range of tools to investigate virtually any cellular process under the microscope. ...This chapter aims to give a general overview on microscope design choices critical for fluorescence live cell imaging that apply to most fluorescence microscopy modalities and on environmental control with a focus on mammalian tissue culture cells. ...
Fluorescence microscopy of live cells has become an integral part of modern cell biology. Fluorescent protein (FP) tags, live
Two-photon directed evolution of green fluorescent proteins.
Stoltzfus CR, et al. Sci Rep 2015. PMID 26145791 Free PMC article.
Directed evolution has been used extensively to improve the properties of a variety of fluorescent proteins (FPs). Evolutionary strategies, however, have not yet been used to improve the two-photon absorption (2PA) properties of a fluorescent protein, properties that are important for two-photon imaging in living tissues, including the brain. Here we demonstrate a technique for quantitatively screening the two-photon excited fluorescence (2PEF) efficiency and 2PA cross section of tens of thousands of mutant FPs expressed in E. coli colonies. ...
Directed evolution has been used extensively to improve the properties of a variety of fluorescent proteins (FPs). Evolutionar …
An auxin-based degron system for the rapid depletion of proteins in nonplant cells.
Nishimura K, et al. Nat Methods 2009. PMID 19915560
The AID system allowed rapid and reversible degradation of target proteins in response to auxin and enabled us to generate efficient conditional mutants of essential proteins in yeast as well as cell lines derived from chicken, mouse, hamster, monkey and human cells, thus offering a powerful tool to control protein expression and study protein function....
The AID system allowed rapid and reversible degradation of target proteins in response to auxin and enabled us to generate efficient …
A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum.
Shaner NC, et al. Nat Methods 2013. PMID 23524392 Free PMC article.
We report a monomeric yellow-green fluorescent protein, mNeonGreen, derived from a tetrameric fluorescent protein from the cephalochordate Branchiostoma lanceolatum. mNeonGreen is the brightest monomeric green or yellow fluorescent protein yet described to our knowledge, performs exceptionally well as a fusion tag for traditional imaging as well as stochastic single-molecule superresolution imaging and is an excellent fluorescence resonance energy transfer (FRET) acceptor for the newest cyan fluorescent proteins....
We report a monomeric yellow-green fluorescent protein, mNeonGreen, derived from a tetrameric fluorescent protein from …
Development and Applications of Superfolder and Split Fluorescent Protein Detection Systems in Biology.
Pedelacq JD and Cabantous S. Int J Mol Sci 2019 - Review. PMID 31311175 Free PMC article.
Molecular engineering of the green fluorescent protein (GFP) into a robust and stable variant named Superfolder GFP (sfGFP) has revolutionized the field of biosensor development and the use of fluorescent markers in diverse area of biology. sfGFP-based self-associating bipartite split-FP systems have been widely exploited to monitor soluble expression in vitro, localization, and trafficking of proteins in cellulo. ...
Molecular engineering of the green fluorescent protein (GFP) into a robust and stable variant named Superfolder GFP (sfGFP) ha …
Membranes of unification
Nick P. Protoplasma 2017. PMID 27900484
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