Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation

Search Page

Filters

My Custom Filters

Results by year

Table representation of search results timeline featuring number of search results per year.

Year Number of Results
2014 1
2016 2
2017 6
2018 12
2019 27
2020 26
2021 58
2022 78
2023 122
2024 114
2025 143

Publication date

Text availability

Article attribute

Article type

Additional filters

Article Language

Species

Sex

Age

Other

Search Results

540 results

Results by year

Filters applied: Abstract, in the last 10 years. Clear all
Page 1
Spatial ecostructural modelling of endometrial cancer identifies the key role of CD90 + CD105 + endothelial cells in tumour heterogeneity and predicts disease recurrence.
Wu D, Lv C, Yan Z, Zhao L, Li L, Ye M, Wang M, Zhai Q, Wang N, Wang Z, Meng Y, Li M. Wu D, et al. Exp Hematol Oncol. 2025 Nov 17;14(1):132. doi: 10.1186/s40164-025-00724-6. Exp Hematol Oncol. 2025. PMID: 41243106 Free article.
METHODS: Single-cell resolution analysis of 40 endometrial cancer cases across four molecular subtypes was performed using imaging mass cytometry (IMC) to quantify the frequency, spatial distribution, and intercellular crosstalk of distinct immune and stromal …
METHODS: Single-cell resolution analysis of 40 endometrial cancer cases across four molecular subtypes was performed using imaging …
Visualizing the co-development of structural and immune cells in the human fetal lung using imaging mass cytometry.
Jia L, Chuva De Sousa Lopes SM, van Unen V, Hiemstra PS, Stolk J, Koning F, Khedoe P. Jia L, et al. Am J Physiol Lung Cell Mol Physiol. 2025 Nov 14. doi: 10.1152/ajplung.00246.2025. Online ahead of print. Am J Physiol Lung Cell Mol Physiol. 2025. PMID: 41236889 Free article.
The co-development and interactions between structural and immune cells in the human fetal lung can be studied using transcriptomic and proteomic approaches. Here, we utilized imaging mass cytometry with a 31-antibody panel to visualize immune and structural …
The co-development and interactions between structural and immune cells in the human fetal lung can be studied using transcriptomic and prot …
Mapping B cells and the immune landscape of tertiary lymphoid structures reveals their clinical impact in neuroblastoma.
Melaiu O, Chierici M, Gragera P, Lazzaro N, Petrilli LL, Wienke J, Bergsma FJ, Verhoeven BM, De Stefanis C, D'Oria V, Benedetti MC, Barillari G, Alaggio R, De Ioris MA, Vinci M, Baryawno N, Carsetti R, Jurman G, Molenaar JJ, Locatelli F, Fruci D. Melaiu O, et al. J Immunother Cancer. 2025 Nov 11;13(11):e012860. doi: 10.1136/jitc-2025-012860. J Immunother Cancer. 2025. PMID: 41218854 Free PMC article.
Spatial organization and functional status of immune cells within TLSs were assessed by imaging mass cytometry using a 29-antibody panel. In parallel, gene expression profiles were obtained through NanoString PanCancer Immune Profiling and further validated u …
Spatial organization and functional status of immune cells within TLSs were assessed by imaging mass cytometry using a …
CD3+ immune cell endotypes are associated with PsA disease phenotype and response to advanced therapy: an integrated mass cytometry and proteomics cohort study.
Eder L, Dang S, Caucheteux SM, Li X, Thib S, Ganatra D, Ritchlin C, Jurisica I, Piguet V, Diao L, Chandran V. Eder L, et al. Ann Rheum Dis. 2025 Nov 7:S0003-4967(25)04450-4. doi: 10.1016/j.ard.2025.10.011. Online ahead of print. Ann Rheum Dis. 2025. PMID: 41206244 Free article.
Proteomic profiling via an aptamer-based assay compared differentially expressed proteins across clusters, followed by pathway analysis. Imaging mass cytometry analysis was performed to characterise T cell subsets in synovial tissue samples. ...
Proteomic profiling via an aptamer-based assay compared differentially expressed proteins across clusters, followed by pathway analysis. …
Functional genomics and tumor microenvironment analysis reveal prognostic biological subtypes in Mantle cell lymphoma.
Sharma S, Ali R, Bouska A, Jochum D, Kesireddy M, Mahov S, Lownik J, Zhang W, Lone W, Soma MA, Gamboa A, Devarakonda V, El-Gamal D, Fariha A, Mansoor A, Stewart D, Martin P, Link BK, Advani RH, Barr PM, Goy AH, Mehta A, Kamdar M, Stephens DM, Bachanova V, Smith L, Morin R, Pararajalingam P, Lunning MA, Fu K, Weisenburger D, Chan WC, Khoury J, Greiner TC, Vose JM, Merchant A, Bi C, Iqbal J; North American Mantle Cell Lymphoma Project (NAMCLP). Sharma S, et al. Nat Commun. 2025 Nov 5;16(1):9762. doi: 10.1038/s41467-025-64666-7. Nat Commun. 2025. PMID: 41193463 Free PMC article.
Multi-proteomic spatial profiling using imaging mass cytometry (IMC) demonstrates enrichment of CD4+ T cells with high expression of exhaustion markers and a dominant population of myeloid cells skewed toward an M2-like phenotype. ...
Multi-proteomic spatial profiling using imaging mass cytometry (IMC) demonstrates enrichment of CD4+ T cells with high …
High-resolution imaging mass cytometry to map subcellular structures.
Bollhagen A, Whipman J, Coelho R, Heinzelmann-Schwarz V, Jacob F, Bodenmiller B. Bollhagen A, et al. Nat Methods. 2025 Oct 30. doi: 10.1038/s41592-025-02889-8. Online ahead of print. Nat Methods. 2025. PMID: 41168486
Imaging mass cytometry (IMC) is a powerful multiplexed imaging technology used to investigate cell phenotypes and spatial organization of tissue in health and disease. ...
Imaging mass cytometry (IMC) is a powerful multiplexed imaging technology used to investigate cell phenotypes and spati …
Spatially resolved airway niches: mapping epithelial-immune microenvironments in asthma.
Sakkal M, Jarab AS, Al Meslamani AZ. Sakkal M, et al. Expert Rev Respir Med. 2025 Oct 29:1-14. doi: 10.1080/17476348.2025.2582244. Online ahead of print. Expert Rev Respir Med. 2025. PMID: 41146609 Review.
We synthesize niches revealed by Visium-HD, Xenium, CosMx SMI, MERFISH, Imaging Mass Cytometry, MIBI, and high-resolution MALDI-MSI - IL-13-rich goblet-cell hubs, mast-cell - smooth-muscle loops, IL-17-driven neutrophil pockets, and fibroblast-remodeling zone …
We synthesize niches revealed by Visium-HD, Xenium, CosMx SMI, MERFISH, Imaging Mass Cytometry, MIBI, and high-resoluti …
Meningeal lymphatic architecture and drainage dynamics surrounding the human middle meningeal artery.
Albayram M, Richmond SB, Yagmurlu K, Tuna IS, Karakaya E, Ravichandran H, Tufan F, Lesha E, Mut M, Bunyak F, Kalani YS, Ergul A, Seidler RD, Albayram O. Albayram M, et al. iScience. 2025 Oct 4;28(11):113693. doi: 10.1016/j.isci.2025.113693. eCollection 2025 Nov 21. iScience. 2025. PMID: 41142129 Free PMC article.
To validate these findings, we performed the high-resolution spatial mapping of the dorsal and ventral dura using immunofluorescence and imaging mass cytometry, identifying PROX1+, PDPN+, and LYVE1+ lymphatic-like structures that are aligned along the MMA. .. …
To validate these findings, we performed the high-resolution spatial mapping of the dorsal and ventral dura using immunofluorescence and …
Pan-cancer convergence of tumour-immune microenvironment motifs revealed by CyTOF and imaging mass cytometry.
Vallée A, Drezet A, Arutkin M. Vallée A, et al. Front Immunol. 2025 Oct 6;16:1672312. doi: 10.3389/fimmu.2025.1672312. eCollection 2025. Front Immunol. 2025. PMID: 41122166 Free PMC article. Review.
Mass cytometry (CyTOF) and Imaging Mass Cytometry (IMC) provide single-cell resolution for over 50 protein markers, enabling unprecedented exploration of tumour and immune heterogeneity. ...
Mass cytometry (CyTOF) and Imaging Mass Cytometry (IMC) provide single-cell resolution for over 50 protein markers, ena …
Neoadjuvant immunotherapy in mismatch-repair-proficient colon cancers.
Tan PB, Verschoor YL, van den Berg JG, Balduzzi S, Kok NFM, Ijsselsteijn ME, Moore K, Jurdi A, Tin A, Kaptein P, van Leerdam ME, Haanen JBAG, Voest EE, de Miranda NFCC, Schumacher TN, Wessels LFA, Chalabi M. Tan PB, et al. Nature. 2025 Oct 20. doi: 10.1038/s41586-025-09679-4. Online ahead of print. Nature. 2025. PMID: 41115454
Furthermore, responding tumours had significantly higher baseline expression of proliferation signatures and TCF1, and imaging mass cytometry revealed a higher percentage of Ki-67(+) cancer and Ki-67(+) CD8(+) T cells in responders compared to non-responders. …
Furthermore, responding tumours had significantly higher baseline expression of proliferation signatures and TCF1, and imaging mas …
Entheseal tissue signature in response to IL-17A inhibition in psoriatic arthritis: results from the EBIO entheseal biopsy study.
Raimondo MG, Mohammadian H, Rauber S, Alivernini S, Fedorchenko V, Rigau AR, Angeli MR, Fagni F, Corte G, Tascilar K, Labinsky H, Ramming AM, Roemer F, Rech J, Braeuer L, D'Agostino MA, Schett G, Pachowsky M, Kleyer A, Ramming A. Raimondo MG, et al. Ann Rheum Dis. 2025 Oct 16:S0003-4967(25)04372-9. doi: 10.1016/j.ard.2025.08.032. Online ahead of print. Ann Rheum Dis. 2025. PMID: 41107119 Free article.
METHODS: In this prospective, interventional phase 4 trial, we enrolled 10 patients with enthesitis of the lateral epicondyle, performed entheseal biopsies, and analysed tissues by imaging mass cytometry (IMC) and spatial transcriptomics before and after trea …
METHODS: In this prospective, interventional phase 4 trial, we enrolled 10 patients with enthesitis of the lateral epicondyle, performed ent …
Analysis of the tumor microenvironment using imaging mass cytometry data.
Loncova Z, Trajanoski Z, Rieder D. Loncova Z, et al. Methods Cell Biol. 2025;199:63-90. doi: 10.1016/bs.mcb.2025.03.006. Epub 2025 Apr 17. Methods Cell Biol. 2025. PMID: 41106942
Recent advancements in high-dimensional imaging technologies, such as multiplex immunofluorescence imaging, imaging mass cytometry (IMC), and multiplexed ion beam imaging (MIBI), have provided powerful tools to investigate the complexity of the TME. ...Here, …
Recent advancements in high-dimensional imaging technologies, such as multiplex immunofluorescence imaging, imaging mass cy …
Imaging mass cytometry-derived single cell extraction, transformation and phenotyping to analyze immune tumor microenvironments.
Malchiodi ZX, Weiner LM. Malchiodi ZX, et al. Methods Cell Biol. 2025;199:113-127. doi: 10.1016/bs.mcb.2025.03.027. Epub 2025 Apr 22. Methods Cell Biol. 2025. PMID: 41106930
Spatial technologies have been revolutionary in the analysis of intact tissue samples as they allow for in depth investigation of cellular spatial interactions that cannot be elucidated through conventional single-cell methodologies. Imaging mass cytometry (I …
Spatial technologies have been revolutionary in the analysis of intact tissue samples as they allow for in depth investigation of cellular s …
Infiltration characteristics and regulatory mechanisms of CD8+ T lymphocytes in solid tumors: spatial distribution, biological functions, and interactions with the immune microenvironment.
Ouyang P, Zhang J, He X, Yang C, Zeng D, Xu D. Ouyang P, et al. Front Immunol. 2025 Oct 1;16:1661545. doi: 10.3389/fimmu.2025.1661545. eCollection 2025. Front Immunol. 2025. PMID: 41103436 Free PMC article. Review.
Cutting-edge technologies-such as single-cell RNA-sequencing, spatial transcriptomics, imaging mass cytometry, and TCR repertoire profiling-have revealed spatial and functional heterogeneity within intratumoral CD8(+) T cells and informed the design of ration …
Cutting-edge technologies-such as single-cell RNA-sequencing, spatial transcriptomics, imaging mass cytometry, and TCR …
Deciphering the cellular tumor microenvironment landscape in salivary gland carcinomas using multiplexed imaging mass cytometry.
Mayer M, Nachtsheim L, Jansen L, Wolber P, Schmiel M, Morris LGT, Kuo F, Ho AL, Quaas A, Klussmann JP, Arolt C. Mayer M, et al. J Exp Clin Cancer Res. 2025 Oct 13;44(1):288. doi: 10.1186/s13046-025-03551-z. J Exp Clin Cancer Res. 2025. PMID: 41083996 Free PMC article.
METHODS: SGC, including salivary duct carcinomas (SDC), acinic cell, mucoepidermoid, and secretory carcinomas, were analyzed using a 13-marker imaging mass cytometry panel. Multichannel image data from 54 primary cases and nodal metastases were processed to g …
METHODS: SGC, including salivary duct carcinomas (SDC), acinic cell, mucoepidermoid, and secretory carcinomas, were analyzed using a 13-mark …
Spatial transcriptomics and immunophenotyping uncover chronic inflammation-induced immune adaptations favoring dysplasia development in patients at risk of colitis-associated cancer.
Frigerio S, Khan HN, Amini M, Mol B, Neefjes-Borst A, Wildenberg ME, Ponsioen CY, D'haens GR, Vercoulen Y, Grootjans J. Frigerio S, et al. J Crohns Colitis. 2025 Oct 13:jjaf184. doi: 10.1093/ecco-jcc/jjaf184. Online ahead of print. J Crohns Colitis. 2025. PMID: 41081629
METHODS: This cohort study utilized GeoMx digital spatial profiling and imaging mass cytometry in 11 patients with either CAC or sporadic CRC (SCRC). Results from this discovery cohort were validated using IHC/IF in an independent cohort of CAC and SCRC patie …
METHODS: This cohort study utilized GeoMx digital spatial profiling and imaging mass cytometry in 11 patients with eith …
Imaging Mass Cytometry-Based Immune Profiling of Human Peyer's Patches in Patients with Crohn's Disease.
Huck A, Rodriguez-Sillke Y, Bojarski C, Kunkel D, Kühl AA, Lehmann M, Bischoff P, Steinhoff U, Siegmund B, Glauben R. Huck A, et al. Eur J Immunol. 2025 Oct;55(10):e70071. doi: 10.1002/eji.70071. Eur J Immunol. 2025. PMID: 41054006 Free PMC article.
Lymphoid follicles in the human gut are critical immune hubs, yet their role in Crohn's disease pathogenesis remains poorly understood. Here, we apply multiplexed imaging mass cytometry to spatially profile Peyer's patches and lymphoid follicles in biopsies f …
Lymphoid follicles in the human gut are critical immune hubs, yet their role in Crohn's disease pathogenesis remains poorly understood. Here …
Multiplexed Imaging Mass Cytometry Reveals Tumor-immune Microenvironment-dependent Hormone Receptor Expression in Adult-Type Ovarian Granulosa Cell Tumors.
Khlebus EY, Vuttaradhi VK, Ferri-Borgogno S, Brodsky AL, Lawson BC, Mok SC, Hillman RT. Khlebus EY, et al. Cancer Res Commun. 2025 Oct 1;5(10):1894-1909. doi: 10.1158/2767-9764.CRC-25-0333. Cancer Res Commun. 2025. PMID: 41042551 Free PMC article.
To elucidate spatial features and cellular interactions within the AGCT tumor microenvironment, we applied imaging mass cytometry using a 34-marker panel on 130 regions from 24 AGCT samples, profiling more than 900,000 single cells. ...
To elucidate spatial features and cellular interactions within the AGCT tumor microenvironment, we applied imaging mass cyt …
Tumor-specific major histocompatibility-II expression predicts pathological complete response to atezolizumab combined to chemotherapy in triple-negative breast cancer.
Balko JM, Licata L, Wang XQ, Dugo M, Huang CS, Egle D, Bermejo B, Zamagni C, Thill M, Anton A, Russo S, Sevillano E, Ciruelos EM, Greil R, Semiglazov V, Colleoni M, Kelly CM, Mariani G, Del Mastro L, Zambelli S, Viale G, Callari M, Viale G, Pusztai L, Gianni L, Ali HR, Bianchini G. Balko JM, et al. NPJ Breast Cancer. 2025 Sep 29;11(1):103. doi: 10.1038/s41523-025-00828-6. NPJ Breast Cancer. 2025. PMID: 41022779 Free PMC article.
We performed biomarker analysis of the phase III NeoTRIP trial where patients were randomized to neoadjuvant carboplatin and nab-paclitaxelatezolizumab. Imaging mass cytometry was used to assess tsMHC-II expression in tumor samples. TsMHC-II positivity was pr …
We performed biomarker analysis of the phase III NeoTRIP trial where patients were randomized to neoadjuvant carboplatin and nab-paclitaxela …
Impact of Fusobacterium nucleatum on immune cell interactions and gene expression in colorectal cancer: a retrospective cohort study.
Heregger R, Huemer F, Greil R, Canadas-Ortega M, Posselt G, Ijsselsteijn ME, Plattner C, Rieder D, Trajanoski Z, Krogsdam A, Klieser E, Neureiter D, Wessler S, Weiss L. Heregger R, et al. Front Immunol. 2025 Sep 11;16:1629014. doi: 10.3389/fimmu.2025.1629014. eCollection 2025. Front Immunol. 2025. PMID: 41019049 Free PMC article.
Spatial immune cell interactions within the tumor microenvironment were characterized using imaging mass cytometry (IMC) and quantified through Voronoi tessellation-derived mixing scores. ...
Spatial immune cell interactions within the tumor microenvironment were characterized using imaging mass cytometry (IMC …
CD206+CD14- Skin-resident Macrophages and DC-T Cell clusters are spatial features characterizing non-Relapsing Cutaneous Squamous Cell Carcinoma.
Elaldi R, Meghraoui-Kheddar A, Elaldi A, Petti L, Gouleau A, Hemon P, Khatir W, Meziane J, Descombes X, Montaudié H, Boyer J, Bozec A, Poissonnet G, Pers JO, Sudaka A, Braud VM, Anjuere F. Elaldi R, et al. Cancer Immunol Res. 2025 Sep 26. doi: 10.1158/2326-6066.CIR-25-0182. Online ahead of print. Cancer Immunol Res. 2025. PMID: 41003664
This underscores the need for molecular signatures capable of stratifying patients during primary tumor resection. Here, we used high-dimensional imaging mass cytometry (IMC) and a 39-antibody panel to define the immune landscape of twenty primary cSCC with d …
This underscores the need for molecular signatures capable of stratifying patients during primary tumor resection. Here, we used high-dimens …
Oncogenic driver mutations underlie the spatial tumour immune landscape of non-small cell lung cancer.
Hartner S, Abolfathi H, Rezanejad M, Liu B, Karimi E, Rogers D, Sorin M, Doré S, Desharnais L, Orain M, Enlow W, Gagné A, Wei Y, Bossé Y, Quail DF, Joubert P, Walsh LA. Hartner S, et al. Nat Commun. 2025 Sep 25;16(1):8402. doi: 10.1038/s41467-025-63465-4. Nat Commun. 2025. PMID: 40998804 Free PMC article.
Here, we investigate the impact of driver mutations on the composition and spatial architecture of the TME in LUAD. Using imaging mass cytometry (IMC), we analyse tumour samples from 157 LUAD patients, integrating genomic and clinical data to link specific mu …
Here, we investigate the impact of driver mutations on the composition and spatial architecture of the TME in LUAD. Using imaging …
A spatial map of MAPK-activated immunosuppressive myeloid populations in pediatric low-grade glioma.
Andrade AF, Sigaud R, Puligandla E, Liu B, Karimi E, Annett A, Rezanejad M, Jawhar W, Taylor R, Cao Y, Schmid S, Selt F, Hernáiz Driever P, Horn S, Sievers P, Prinz M, Glatzel M, Mawrin C, Hartmann C, Monoranu CM, Konnikova L, Sahm F, Pfister SM, Witt O, Jones DTW, Koch A, Kleinman CL, Capper D, Walsh L, Jabado N, Milde T. Andrade AF, et al. Nat Immunol. 2025 Oct;26(10):1794-1806. doi: 10.1038/s41590-025-02268-7. Epub 2025 Sep 15. Nat Immunol. 2025. PMID: 40954250
Pediatric low-grade gliomas (pLGGs) are mitogen-activated protein kinase (MAPK) pathway-activated brain tumors prevalent in children and are associated with morbidity despite favorable survival. Here using imaging mass cytometry, we spatially characterized at …
Pediatric low-grade gliomas (pLGGs) are mitogen-activated protein kinase (MAPK) pathway-activated brain tumors prevalent in children and are …
High-plex imaging of hepatoblastoma and adjacent liver in pediatric patients reveals a predominant myeloid infiltrate expressing immune-checkpoints.
Krijgsman D, Schubert SA, Kraaier LJ, Lu Y, van Avondt K, Verdonschot MEL, Leusen JHW, van den Heuvel MC, de Kleine RH, de Meijer VE, Kraal KCJ, de Krijger RR, Zsiros J, Peng WC, Vercoulen Y. Krijgsman D, et al. Cancer Immunol Immunother. 2025 Sep 13;74(10):310. doi: 10.1007/s00262-025-04164-3. Cancer Immunol Immunother. 2025. PMID: 40944713 Free PMC article.
METHODS: To investigate the tissue landscape, a high-plex imaging mass cytometry panel was developed and applied to tissue of treatment-naive HB and chemotherapy-treated HB, with paired adjacent normal liver tissue. ...
METHODS: To investigate the tissue landscape, a high-plex imaging mass cytometry panel was developed and applied to tis …
Multiplex Imaging Mass Cytometry Reveals Prognostic Immunosuppressive Subpopulations and Macrophage-Driven Metastasis in Osteosarcoma.
Gyau BB, Wang J, Wu W, Scull B, Major AM, Jin W, Cates JMM, Hicks J, Man TK. Gyau BB, et al. Cancers (Basel). 2025 Aug 26;17(17):2780. doi: 10.3390/cancers17172780. Cancers (Basel). 2025. PMID: 40940877 Free PMC article.
Despite immunotherapy advancements, its efficacy in OS is hindered by an incomplete understanding of the immunosuppressive tumor microenvironment (TME). Methods: We utilized multiplex imaging mass cytometry and phenoplexing to characterize immune and stromal …
Despite immunotherapy advancements, its efficacy in OS is hindered by an incomplete understanding of the immunosuppressive tumor microenviro …
Multi-modal image analysis for large-scale cancer tissue studies within IMMUcan.
Eling N, Dorier J, Rusakiewicz S, Liechti R, Devanand P, Daniel M, Windhager J, Fernandez BP, Déglise S, Despland L, Benyagoub A, Możejko M, Uchal D, Szczurek E, Loboda A, Sandkuijl D, Parsotam N, Hong HS, Morfouace M, Guex N, Coukos G, Bodenmiller B, Tissot S, Schulz D. Eling N, et al. Cell Rep Methods. 2025 Sep 15;5(9):101170. doi: 10.1016/j.crmeth.2025.101170. Epub 2025 Sep 9. Cell Rep Methods. 2025. PMID: 40930089 Free PMC article.
Here, we describe and compare two workflows for multiplexed immunofluorescence (mIF) and imaging mass cytometry (IMC) developed within IMMUcan to enable the generation of standardized data for cancer tissue analysis. ...
Here, we describe and compare two workflows for multiplexed immunofluorescence (mIF) and imaging mass cytometry (IMC) d …
Tumor microenvironment differences between diagnostic and relapsed classic Hodgkin lymphoma revealed by scRNA-seq.
Yin Y, Rai S, Jiang A, Xu AM, O'Brien L, Telenius A, Delabie J, Chong LC, Hung SS, Merchant AA, Scott DW, Savage KJ, Aoki T, Steidl C. Yin Y, et al. Blood Adv. 2025 Sep 9:bloodadvances.2025017107. doi: 10.1182/bloodadvances.2025017107. Online ahead of print. Blood Adv. 2025. PMID: 40924921
Cell-cell interaction analysis revealed the importance of interactions between LGALS9+ naive B cells and HAVCR2+ Tregs in the early-relapse setting. Spatial analysis by imaging mass cytometry confirmed close proximity of Galectin-9+ naive B cells with TIM-3+ …
Cell-cell interaction analysis revealed the importance of interactions between LGALS9+ naive B cells and HAVCR2+ Tregs in the early-relapse …
Intestinal epithelial Ceacam1 deficiency prevents steroid-refractory acute gut graft-versus-host disease.
Song Q, Zheng M, Li Q, Wu X, Lin B, Kang TH, Qin H, Kujawski M, Pillai RK, Lin JL, Nakamura R, Shively J, Martin PJ, Zeng D. Song Q, et al. JCI Insight. 2025 Sep 9;10(17):e186984. doi: 10.1172/jci.insight.186984. eCollection 2025 Sep 9. JCI Insight. 2025. PMID: 40923321 Free PMC article.
CEACAM1 on intestinal epithelial cells (IECs) is proposed to regulate bacterial translocation and subsequent immune responses in the intestine. Here, with imaging mass cytometry (IMC), combined scRNA-Seq with ATAC-Seq, and high-dimensional flow cytometry anal …
CEACAM1 on intestinal epithelial cells (IECs) is proposed to regulate bacterial translocation and subsequent immune responses in the intesti …
Imaging mass cytometry dataset of small-cell lung cancer tumors and tumor microenvironments.
Rose F, Ibruli O, Lichius L, Kiljan M, Gozum G, Caiaffa MI, Cai J, Niu LN, Herter JM, Grüll H, Büttner R, Beleggia F, Bosco G, George J, Herter-Sprie GS, Reinhardt HC, Bozek K. Rose F, et al. BMC Res Notes. 2025 Sep 8;18(1):385. doi: 10.1186/s13104-025-07460-4. BMC Res Notes. 2025. PMID: 40922010 Free PMC article.
To investigate tumor composition, spatial architecture, and interactions with the surrounding microenvironment, we acquired multiplexed images of mouse lung tumors using imaging mass cytometry (IMC). These data build upon a previously published characterizati …
To investigate tumor composition, spatial architecture, and interactions with the surrounding microenvironment, we acquired multiplexed imag …
Spatial profiling of longitudinal glioblastoma reveals consistent changes in cellular architecture, post-treatment.
Ajaib S, Winter-Luke J, Digby RJ, Pollock S, Hemmings G, Gusnanto A, Chakrabarty A, Ismail A, Wilson E, Hunter B, Filby A, McDonald D, Brockman AA, Ihrie RA, Stead LF. Ajaib S, et al. Neuro Oncol. 2025 Sep 1:noaf190. doi: 10.1093/neuonc/noaf190. Online ahead of print. Neuro Oncol. 2025. PMID: 40898767 Free article.
METHODS: To explore these relationships, we employed imaging mass cytometry to measure the expression of 34 protein markers, enabling the identification of GBM-specific cell types and their interactions at single-cell protein level in paired primary (pre-trea …
METHODS: To explore these relationships, we employed imaging mass cytometry to measure the expression of 34 protein mar …
Skin biopsies enhance prediction of clinical trajectory in diffuse cutaneous systemic sclerosis.
Lakin KS, Spivack J, Gordon JK, Zhang Y, Jannat-Khah D, Ravichandran H, Anandasabapathy N, Orange DE, Spiera RF. Lakin KS, et al. Arthritis Rheumatol. 2025 Sep 1. doi: 10.1002/art.43370. Online ahead of print. Arthritis Rheumatol. 2025. PMID: 40888138
Fibroblast spatial organization was visualized using imaging mass cytometry (IMC) in a representative sample. RESULTS: Despite similar baseline mRSS, early disease was associated with lower CD34, higher aSMA, increased B cells, and greater mRSS improvement co …
Fibroblast spatial organization was visualized using imaging mass cytometry (IMC) in a representative sample. RESULTS: …
DNA damage response profile distinguishes poor-acting gliomas with shared methylome signatures.
Leelatian N, Singh C, Bouffard R, Sundaram RK, Diggins KE, Sullivan W, Paradkar S, Omay ZE, Mobley BC, Gueble SE, Vasquez JC, Bindra RS. Leelatian N, et al. Neuro Oncol. 2025 Aug 27:noaf199. doi: 10.1093/neuonc/noaf199. Online ahead of print. Neuro Oncol. 2025. PMID: 40887772
METHODS: We characterized DDR epigenetics and gene expression of TCGA glioblastomas (GBM) and low-grade gliomas (LGG). Single-cell protein analysis by imaging mass cytometry (IMC) was performed on a separate cohort of 118 diffuse gliomas. ...
METHODS: We characterized DDR epigenetics and gene expression of TCGA glioblastomas (GBM) and low-grade gliomas (LGG). Single-cell protein a …
Investigating cytotoxic and inflammatory human IL-7 receptor low effector memory CD8+ T cells in lupus.
Lee SJ, Shin MS, Lee YJ, Osmani L, Seong WJ, Cai H, Par-Young J, Ahn JG, Park HJ, Kim M, Unlu S, Kim H, Han MH, Dong X, You S, Lee EB, Kang I. Lee SJ, et al. EBioMedicine. 2025 Sep;119:105898. doi: 10.1016/j.ebiom.2025.105898. Epub 2025 Aug 25. EBioMedicine. 2025. PMID: 40857939 Free PMC article.
METHODS: Our own and publicly available RNA-seq and microarray data from the peripheral blood and kidney tissues of patients with lupus were analysed. Imaging Mass Cytometry (IMC) analysis of immune cells was conducted in lupus and normal kidney tissues. ...
METHODS: Our own and publicly available RNA-seq and microarray data from the peripheral blood and kidney tissues of patients with lupus were …
Blood immunomap for prediction of responses to anti-PD-1 immunotherapy in metastatic non-small cell lung cancer.
Semitekolou M, Paschalidis N, Lo Tartaro D, Tsitsopoulou A, Stamou P, Mavroudis A, Markaki E, Varveri A, Morianos I, Lavigne M, Fotsitzoudis C, Magkouta S, Dede K, Kalomenidis I, Samitas K, Potaris K, Cossarizza A, Mavroudis D, De Biasi S, Verginis P. Semitekolou M, et al. iScience. 2025 Jun 2;28(9):112804. doi: 10.1016/j.isci.2025.112804. eCollection 2025 Sep 19. iScience. 2025. PMID: 40860257 Free PMC article.
We identified heightened frequencies of CD8(+)PD-L1(+) T cells in non-responders compared to responders. Notably, imaging mass cytometry data revealed that CD8(+)PD-L1(+) T cells were enriched in tumor biopsies, pleural infusions, and BAL of early-stage NSCLC …
We identified heightened frequencies of CD8(+)PD-L1(+) T cells in non-responders compared to responders. Notably, imaging mass …
Spatial mapping of rheumatoid arthritis synovial niches reveals a LYVE1+ macrophage network associated with response to therapy.
De Lima J, Boutet MA, Bortolotti O, Chépeaux LA, Glasson Y, Dumé AS, Lau R, Humbert P, Allain S, Le Pluart A, Nerviani A, Fossati-Jimack L, Michaud HA, Guicheux J, Le Goff B, Lewis MJ, Pitzalis C, Courties G, Apparailly F, Blanchard F. De Lima J, et al. Ann Rheum Dis. 2025 Aug 22:S0003-4967(25)04292-X. doi: 10.1016/j.ard.2025.07.019. Online ahead of print. Ann Rheum Dis. 2025. PMID: 40849271 Free article.
METHODS: We performed high-dimensional imaging mass cytometry on synovial tissues from healthy controls, patients with osteoarthritis, and patients with early RA longitudinally before and after 6 months of conventional synthetic disease-modifying antirheumati …
METHODS: We performed high-dimensional imaging mass cytometry on synovial tissues from healthy controls, patients with …
Characteristic miRNA profiles represent clinicopathological diversity of small cell lung cancer.
Horie M, Takumida H, Koba H, Ueda T, Tanaka H, Suzuki M, Ito Y, Ito A, Kondo M, Suzuki HI, Matsumoto I, Yano S, Saito A, Maeda D. Horie M, et al. J Pathol. 2025 Oct;267(2):168-180. doi: 10.1002/path.6458. Epub 2025 Aug 22. J Pathol. 2025. PMID: 40842416 Free PMC article.
Comprehensive enhancer profiling using SCLC cell lines indicated that miR-375 and miR-9-5p were regulated by super-enhancers in a subtype-specific manner. Multiplex immunohistochemistry by imaging mass cytometry found that the miR-9-5p-high SCLC was character …
Comprehensive enhancer profiling using SCLC cell lines indicated that miR-375 and miR-9-5p were regulated by super-enhancers in a subtype-sp …
DCLK1 isoform (DCLK1-S) as a critical player in promoting inflammation, tissue remodeling, and EMT in mouse models of colitis.
Yusuf K, Roy BC, Hauser WL, Al-Kasspooles M, Sanchez S, Attard TM, Pei D, Sampath V, Anant S, Umar S. Yusuf K, et al. PLoS Pathog. 2025 Aug 21;21(8):e1013360. doi: 10.1371/journal.ppat.1013360. eCollection 2025 Aug. PLoS Pathog. 2025. PMID: 40839695 Free PMC article.
This study explored the role of the DCLK1 isoform (DCLK1-S) in promoting infectious/chemical colitis by utilizing high-throughput imaging mass cytometry (IMC). METHODS: Transgenic mice were either infected with Citrobacter rodentium (CR) or received DSS and t …
This study explored the role of the DCLK1 isoform (DCLK1-S) in promoting infectious/chemical colitis by utilizing high-throughput imaging …
Biodistribution of nanoplastics in mice: advancing analytical techniques using metal-doped plastics.
Staufer T, Kopatz V, Pradel A, Brodie T, Kuhrwahl R, Stroka D, Wallner J, Kenner L, Pichler V, Grüner F, Mitrano DM. Staufer T, et al. Commun Biol. 2025 Aug 19;8(1):1247. doi: 10.1038/s42003-025-08709-1. Commun Biol. 2025. PMID: 40830574 Free PMC article.
To address this, we propose a workflow for NPs assessment in biological samples combining three complimentary methods, namely inductively coupled plasma mass spectrometry (ICP-MS), X-ray fluorescence imaging (XFI), and imaging mass cytometry (IMC) to detect p …
To address this, we propose a workflow for NPs assessment in biological samples combining three complimentary methods, namely inductively co …
Neoadjuvant Immunotherapy Promotes the Formation of Mature Tertiary Lymphoid Structures in a Remodeled Pancreatic Tumor Microenvironment.
Sidiropoulos DN, Shin SM, Wetzel M, Girgis AA, Bergman D, Danilova L, Perikala S, Shu D, Montagne JM, Deshpande A, Leatherman J, Dequiedt L, Jacobs V, Ogurtsova A, Mo G, Yuan X, Lvovs D, Stein-O'Brien G, Yarchoan M, Zhu Q, Harper EI, Weeraratna AT, Kiemen AL, Jaffee EM, Zheng L, Ho WJ, Anders RA, Fertig EJ, Kagohara LT. Sidiropoulos DN, et al. Cancer Immunol Res. 2025 Nov 3;13(11):1716-1731. doi: 10.1158/2326-6066.CIR-25-0387. Cancer Immunol Res. 2025. PMID: 40815230 Free PMC article.
Using machine learning-enabled hematoxylin and eosin image classification models, imaging mass cytometry, and unsupervised gene expression matrix factorization methods for spatial transcriptomics, we characterized cellular states within and adjacent to TLS sp …
Using machine learning-enabled hematoxylin and eosin image classification models, imaging mass cytometry, and unsupervi …
Resident Macrophage-Orchestrated Immune and Fibroblast Interactions in Immune Checkpoint Inhibitor-Associated Nephrotoxicity.
Ma Y, Chen Y, Yao Q, Wang Y, Ouyang N, Han F, Wang G, Wang M, Yuan Y, Chen J, Shan J, Chen D. Ma Y, et al. Adv Sci (Weinh). 2025 Nov;12(42):e05445. doi: 10.1002/advs.202505445. Epub 2025 Aug 14. Adv Sci (Weinh). 2025. PMID: 40810645
The mechanisms driving ICI-AN remain poorly understood, limiting diagnostic and therapeutic progress. This study integrates imaging mass cytometry, transcriptomics, and murine models to investigate the cellular and molecular mechanisms driving ICI-AN. ...
The mechanisms driving ICI-AN remain poorly understood, limiting diagnostic and therapeutic progress. This study integrates imaging …
Macrophage transfer promotes intestinal mucosal healing by encouraging transit-amplifying cell expansion in mice.
Kang DS, Song YM, Park YJ, Jeong HJ, Hong JJ, Seok SH, Na YR. Kang DS, et al. Front Immunol. 2025 Jul 21;16:1555695. doi: 10.3389/fimmu.2025.1555695. eCollection 2025. Front Immunol. 2025. PMID: 40761793 Free PMC article.
Our findings demonstrate that Tnf(-/-) macrophage transplantation ameliorates weight loss and shortening of colon in a mouse model of colitis. Imaging mass cytometry revealed that Tnf(-/-) macrophages particularly increase the population of transit-amplifying …
Our findings demonstrate that Tnf(-/-) macrophage transplantation ameliorates weight loss and shortening of colon in a mouse model of coliti …
Temporo-spatial cellular atlas of the regenerating alveolar niche in idiopathic pulmonary fibrosis.
Weeratunga P, Hunter B, Sergeant M, Bull J, Clelland C, Denney L, Vuppusetty C, Burgoyne R, Woo J, Hu T, Borthwick L, Shaw J, Antanaviciute A, Filby A, Byrne H, Fisher A, Ho LP. Weeratunga P, et al. Nat Commun. 2025 Aug 4;16(1):7150. doi: 10.1038/s41467-025-61880-1. Nat Commun. 2025. PMID: 40759629 Free PMC article.
Here, using human lung tissue that represents different stages of disease and a 33-plex single-cell imaging mass cytometry (IMC), we present a high-resolution, temporo-spatial cell atlas of the regenerating alveolar niche. ...
Here, using human lung tissue that represents different stages of disease and a 33-plex single-cell imaging mass cytometry …
NK cell-macrophage interactions in granulomas correlate with limited tuberculosis pathology.
Niewold P, IJsselsteijn ME, Dijkman K, de Miranda N, Ottenhoff THM, Verreck FAW, Joosten SA. Niewold P, et al. PLoS Pathog. 2025 Aug 1;21(8):e1012980. doi: 10.1371/journal.ppat.1012980. eCollection 2025 Aug. PLoS Pathog. 2025. PMID: 40749077 Free PMC article.
To identify protective responses in granulomas, imaging mass cytometry was used to study archived lung tissue from low dose Mtb-infected non-human primates presenting with various levels of disease. ...
To identify protective responses in granulomas, imaging mass cytometry was used to study archived lung tissue from low …
Integrative transcriptomic and single-cell protein characterization of colorectal carcinoma delineates distinct tumor immune microenvironments associated with overall survival.
Hissong E, Bhinder B, Kim J, Ohara K, Ravichandran H, Assaad MA, Elsoukkary S, Shusterman M, Khan U, Eng KW, Bareja R, Manohar J, Sigouros M, Rendeiro AF, Jessurun J, Ocean AJ, Sboner A, Elemento O, Mosquera JM, Shah MA. Hissong E, et al. Pathol Res Pract. 2025 Sep;273:156150. doi: 10.1016/j.prp.2025.156150. Epub 2025 Jul 28. Pathol Res Pract. 2025. PMID: 40743578
A subset of tumors underwent in situ analysis of the TIME spatial composition at single-cell resolution through Imaging Mass Cytometry. RESULTS: Gene set enrichment analysis revealed two distinct groups of advanced CRC, one with an immune activated phenotype …
A subset of tumors underwent in situ analysis of the TIME spatial composition at single-cell resolution through Imaging Mass …
Robust consensus nuclear and cell segmentation.
Irfan MO, González-Solares EA, Whitmarsh T, Molaeinezhad A, Al Sa'd M, Mulvey CM, Ribes MP, Fatemi A, Bressan D, Walton NA. Irfan MO, et al. Front Genet. 2025 Jul 15;16:1547788. doi: 10.3389/fgene.2025.1547788. eCollection 2025. Front Genet. 2025. PMID: 40734708 Free PMC article.
Spatial Proximity of Immune Cell Pairs to Cancer Cells in the Tumor Microenvironment as Biomarkers for Patient Stratification.
Li JR, Pan X, Lin Y, Zhao Y, Liu Y, Li Y, Amos CI, Cheng C. Li JR, et al. Cancers (Basel). 2025 Jul 14;17(14):2335. doi: 10.3390/cancers17142335. Cancers (Basel). 2025. PMID: 40723219 Free PMC article.
We hypothesized that the relative proximity of specific immune cell pairs to cancer cells within the TME could help predict their pro- or anti-tumor functions and reflect clinically relevant immune dynamics. METHODS: We analyzed imaging mass cytometry (IMC) d …
We hypothesized that the relative proximity of specific immune cell pairs to cancer cells within the TME could help predict their pro- or an …
Multiplex imaging analysis of the tumor immune microenvironment for guiding precision immunotherapy.
Liu F, Li G, Zheng Y, Liu Y, Liu K. Liu F, et al. Front Immunol. 2025 Jul 11;16:1617906. doi: 10.3389/fimmu.2025.1617906. eCollection 2025. Front Immunol. 2025. PMID: 40718485 Free PMC article. Review.
This mini-review discusses the latest multiplex imaging methods, including Imaging Mass Cytometry (IMC), Multiplexed Ion Beam Imaging (MIBI), Cyclic Immunofluorescence (CycIF), and Digital Spatial Profiling (DSP), emphasizing their roles in identifying spatia …
This mini-review discusses the latest multiplex imaging methods, including Imaging Mass Cytometry (IMC), Multiplexed Io …
Mass In Situ Hybridization Enables Mass Cytometry to Detect Telomere Length.
Yan K, Zhang H, Han G, Kong R, Zhao Y. Yan K, et al. Anal Chem. 2025 Aug 5;97(30):16267-16274. doi: 10.1021/acs.analchem.5c01665. Epub 2025 Jul 23. Anal Chem. 2025. PMID: 40699549
Finally, we successfully applied MISH to detect the telomere length via mass cytometry and imaging mass cytometry (IMC). Our method establishes the first successful strategy for telomere length detection via mass cytometry, marking a significant technological …
Finally, we successfully applied MISH to detect the telomere length via mass cytometry and imaging mass cytometry (IMC) …
540 results