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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1978 1
1981 1
1982 1
1983 1
1984 3
1985 8
1986 5
1987 9
1988 17
1989 43
1990 73
1991 78
1992 122
1993 158
1994 149
1995 228
1996 274
1997 422
1998 629
1999 1049
2000 1479
2001 1789
2002 1827
2003 1913
2004 1879
2005 2059
2006 2142
2007 2516
2008 2499
2009 2784
2010 2905
2011 2850
2012 3030
2013 2790
2014 2465
2015 2380
2016 2032
2017 1770
2018 1539
2019 530
2020 8
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42,300 results
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Page 1
Near-Infrared Fluorescent Proteins: Multiplexing and Optogenetics across Scales.
Shcherbakova DM, et al. Trends Biotechnol 2018 - Review. PMID 30041828 Free PMC article.
Since mammalian tissue is relatively transparent to near-infrared (NIR) light, NIR fluorescent proteins (FPs) engineered from bacterial phytochromes have become widely used probes for non-invasive in vivo imaging. ...
Since mammalian tissue is relatively transparent to near-infrared (NIR) light, NIR fluorescent proteins (FPs) engineered from bacteri …
Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks
Greenwald EC, et al. Chem Rev 2018 - Review. PMID 30550275
The discovery of fluorescent proteins over 25 years ago enabled the development of a diverse array of genetically encodable fluorescent biosensors that are capable of measuring the spatiotemporal dynamics of signal transduction pathways in live cells. ...
The discovery of fluorescent proteins over 25 years ago enabled the development of a diverse array of genetically encodable fluoresce …
A scalable strategy for high-throughput GFP tagging of endogenous human proteins.
Leonetti MD, et al. Proc Natl Acad Sci U S A 2016. PMID 27274053 Free PMC article.
A central challenge of the postgenomic era is to comprehensively characterize the cellular role of the ∼20,000 proteins encoded in the human genome. ...We next present how our protocols can be easily adapted for the tagging of a given target with GFP repeats, critically enabling the study of low-abundance proteins. ...
A central challenge of the postgenomic era is to comprehensively characterize the cellular role of the ∼20,000 proteins encoded in th …
Improved green fluorescence
Heim R, et al. Nature 1995. PMID 7854443
Reporter gene assays
Liu AM, et al. Methods Mol Biol 2009. PMID 19347619
Construction of a linker library with widely controllable flexibility for fusion protein design
Li G, et al. Appl Microbiol Biotechnol 2016. PMID 26394862
Flexibility or rigidity of the linker between two fused proteins is an important parameter that affects the function of fusion proteins. ...Our studies demonstrated that the construction of the linker library with the widely controllable flexibility could provide appropriate linkers with the desirable characteristics to engineer the fusion proteins with the expected functions....
Flexibility or rigidity of the linker between two fused proteins is an important parameter that affects the function of fusion pro
A method for fixing and paraffin embedding tissue to retain the natural fluorescence of reporter proteins.
Nakagawa A, et al. Biotechniques 2015. PMID 26345508 Free article.
We demonstrate the utility of this method for visualizing green and red fluorescent proteins in a wide range of murine tissues using both cytoplasmic and membrane-localized fluorescent reporter proteins. Tissues fixed by this method also allow for immunohistochemical studies, providing a single method to visualize the natural fluorescence of reporter proteins with subsequent detection of cellular proteins....
We demonstrate the utility of this method for visualizing green and red fluorescent proteins in a wide range of murine tissues using …
Chemistry Is Dead. Long Live Chemistry!
Lavis LD. Biochemistry 2017 - Review. PMID 28704030
Chemistry, once king of fluorescence microscopy, was usurped by the field of fluorescent proteins. The increased demands of modern microscopy techniques on the "photon budget" require better and brighter fluorophores, causing a renewed interest in synthetic dyes. ...
Chemistry, once king of fluorescence microscopy, was usurped by the field of fluorescent proteins. The increased demands of modern mi …
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