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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1951 1
1952 4
1953 5
1954 1
1955 3
1956 3
1957 3
1958 4
1960 1
1965 5
1966 13
1967 16
1968 25
1969 21
1970 15
1971 17
1972 21
1973 27
1974 22
1975 15
1976 19
1977 28
1978 15
1979 12
1980 12
1981 16
1982 16
1983 20
1984 9
1985 13
1986 11
1987 9
1988 14
1989 13
1990 9
1991 8
1992 9
1993 12
1994 10
1995 7
1996 8
1997 7
1998 18
1999 12
2000 20
2001 13
2002 9
2003 21
2004 30
2005 25
2006 13
2007 30
2008 45
2009 38
2010 24
2011 41
2012 33
2013 38
2014 23
2015 20
2016 19
2017 17
2018 13
2019 5
2020 1
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951 results
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Page 1
Time-resolved cryo-electron microscopy: Recent progress.
Frank J. J Struct Biol 2017 - Review. PMID 28625887 Free PMC article.
Time-resolved cryo-electron microscopy (cryo-EM) combines the known advantages of single-particle cryo-EM in visualizing molecular structure with the ability to dissect the time progress of a reaction between molecules in vitro. ...
Time-resolved cryo-electron microscopy (cryo-EM) combines the known advantages of single-particle cryo-EM in visualizing molec …
Advances in electron microscopy: A qualitative view of instrumentation development for macromolecular imaging and tomography.
Schröder RR. Arch Biochem Biophys 2015 - Review. PMID 26032338
Instrumentation developers, however, are offering yet more novel electron optical devices, such as energy filters and monochromators, aberration correctors or physical phase plates. Here we discuss how current instrumentation has already changed cryo EM, and how newly available instrumentation - often developed in other fields of electron microscopy - may further develop the use and applicability of cryo EM to the imaging of single isolated macromolecules of smaller size or molecules embedded in a crowded cellular environment....
Instrumentation developers, however, are offering yet more novel electron optical devices, such as energy filters and monochro
Correlative fluorescence and electron microscopy.
Schirra RT and Zhang P. Curr Protoc Cytom 2014 - Review. PMID 25271959 Free PMC article.
Correlative fluorescence and electron microscopy (CFEM) is a multimodal technique that combines dynamic and localization information from fluorescence methods with ultrastructural data from electron microscopy, to give new information about how cellular components change relative to the spatiotemporal dynamics within their environment. ...The realization of the advantages of these methods in cell biology has led to rapid improvement in the protocols and has ushered in a new generation of instruments to reach the next level of correlation--integration....
Correlative fluorescence and electron microscopy (CFEM) is a multimodal technique that combines dynamic and localization infor …
New hardware and workflows for semi-automated correlative cryo-fluorescence and cryo-electron microscopy/tomography.
Schorb M, et al. J Struct Biol 2017. PMID 27368127 Free PMC article.
Correlative light and electron microscopy allows features of interest defined by fluorescence signals to be located in an electron micrograph of the same sample. ...Here we present instrumentation, software and an experimental workflow that improves the ease of use, throughput and performance of correlated cryo-fluorescence and cryo-electron microscopy. ...
Correlative light and electron microscopy allows features of interest defined by fluorescence signals to be located in an e
Routine single particle CryoEM sample and grid characterization by tomography
Noble AJ, et al. Elife 2018. PMID 29809143 Free PMC article.
Single particle cryo-electron microscopy (cryoEM) is often performed under the assumption that particles are not adsorbed to the air-water interfaces and in thin, vitreous ice. ...We also show that fiducial-less cryo-electron tomography on single particle grids may be used to determine ice thickness, optimal single particle collection areas and strategies, particle heterogeneity, and de novo models for template picking and single particle alignment....
Single particle cryo-electron microscopy (cryoEM) is often performed under the assumption that particles are not adsorbed to t …
Scanning transmission electron microscopy through-focal tilt-series on biological specimens.
Trepout S, et al. Micron 2015. PMID 26093182
Since scanning transmission electron microscopy can produce high signal-to-noise ratio bright-field images of thick (≥500 nm) specimens, this tool is emerging as the method of choice to study thick biological samples via tomographic approaches. ...
Since scanning transmission electron microscopy can produce high signal-to-noise ratio bright-field images of thick (≥500 nm) …
Optimizing "self-wicking" nanowire grids
Wei H, et al. J Struct Biol 2018. PMID 29317278 Free PMC article.
We have developed a self-blotting TEM grid for use with a novel instrument for vitrifying samples for cryo-electron microscopy (cryoEM). ...
We have developed a self-blotting TEM grid for use with a novel instrument for vitrifying samples for cryo-electron microsc
Improved Zernike-type phase contrast for transmission electron microscopy.
Koeck PJ. J Microsc 2015. PMID 25865092 Free article.
Zernike phase contrast has been recognized as a means of recording high-resolution images with high contrast using a transmission electron microscope. ...According to the original proposal discussed in Danev and Nagayama (2001) and references therein, the Zernike phase plate applies a phase shift of π/2 to all scattered electron beams outside a given scattering angle and an image is recorded at Gaussian focus or slight underfocus (below Scherzer defocus). ...
Zernike phase contrast has been recognized as a means of recording high-resolution images with high contrast using a transmission electro
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