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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1946 2
1947 13
1948 5
1949 3
1950 14
1951 13
1952 17
1953 14
1954 17
1955 24
1956 14
1957 23
1958 23
1959 18
1960 21
1961 38
1962 45
1963 152
1964 272
1965 361
1966 346
1967 410
1968 453
1969 451
1970 491
1971 595
1972 564
1973 568
1974 676
1975 388
1976 236
1977 238
1978 224
1979 288
1980 376
1981 417
1982 400
1983 439
1984 470
1985 457
1986 431
1987 407
1988 509
1989 524
1990 668
1991 924
1992 887
1993 847
1994 954
1995 950
1996 886
1997 1010
1998 1224
1999 1407
2000 1683
2001 2618
2002 2651
2003 3061
2004 3590
2005 3582
2006 3120
2007 3310
2008 3371
2009 3661
2010 3889
2011 4086
2012 4154
2013 4076
2014 3733
2015 3918
2016 3532
2017 2956
2018 2227
2019 1063
2020 29
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79,536 results
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Page 1
Fluorescence microscopy.
Sanderson MJ, et al. Cold Spring Harb Protoc 2014 - Review. PMID 25275114 Free PMC article.
Fluorescence microscopy is a major tool with which to monitor cell physiology. Although the concepts of fluorescence and its optical separation using filters remain similar, microscope design varies with the aim of increasing image contrast and spatial resolution. The basics of wide-field microscopy are outlined to emphasize the selection, advantages, and correct use of laser scanning confocal microscopy, two-photon microscopy, scanning disk confocal microscopy, total internal reflection, and super-resolution microscopy. ...
Fluorescence microscopy is a major tool with which to monitor cell physiology. Although the concepts of fluorescence an
Fluorescence microscopy.
Lichtman JW and Conchello JA. Nat Methods 2005 - Review. PMID 16299476
Although fluorescence microscopy permeates all of cell and molecular biology, most biologists have little experience with the underlying photophysical phenomena. Understanding the principles underlying fluorescence microscopy is useful when attempting to solve imaging problems. Additionally, fluorescence microscopy is in a state of rapid evolution, with new techniques, probes and equipment appearing almost daily. ...
Although fluorescence microscopy permeates all of cell and molecular biology, most biologists have little experience with the …
A guide to light-sheet fluorescence microscopy for multiscale imaging.
Power RM and Huisken J. Nat Methods 2017 - Review. PMID 28362435
The impact of light-sheet fluorescence microscopy (LSFM) is visible in fields as diverse as developmental and cell biology, anatomical science, biophysics and neuroscience. ...
The impact of light-sheet fluorescence microscopy (LSFM) is visible in fields as diverse as developmental and cell biology, an …
Advanced fluorescence microscopy techniques--FRAP, FLIP, FLAP, FRET and FLIM.
Ishikawa-Ankerhold HC, et al. Molecules 2012 - Review. PMID 22469598 Free PMC article.
Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity. ...First, a brief introduction into the mechanisms underlying fluorescence as a physical phenomenon and fluorescence, confocal, and multiphoton microscopy is given. ...
Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility,
Single-molecule fluorescence microscopy review: shedding new light on old problems.
Shashkova S and Leake MC. Biosci Rep 2017 - Review. PMID 28694303 Free PMC article.
Improvements in detector and dye technologies coupled to advances in image analysis methods have fuelled recent development towards single-molecule fluorescence microscopy, which can utilize light microscopy tools to enable the faithful detection and analysis of single fluorescent molecules used as reporter tags in biological samples. ...In this review, we discuss some of the recent discoveries in the life sciences which have been enabled using single-molecule fluorescence microscopy, paying particular attention to the so-called 'super-resolution' fluorescence microscopy techniques in live cells, which are at the cutting-edge of these methods. ...
Improvements in detector and dye technologies coupled to advances in image analysis methods have fuelled recent development towards single-m …
Super-resolution fluorescence microscopy.
Huang B, et al. Annu Rev Biochem 2009 - Review. PMID 19489737 Free PMC article.
Achieving a spatial resolution that is not limited by the diffraction of light, recent developments of super-resolution fluorescence microscopy techniques allow the observation of many biological structures not resolvable in conventional fluorescence microscopy. ...It is anticipated that super-resolution fluorescence microscopy will become a widely used tool for cell and tissue imaging to provide previously unobserved details of biological structures and processes....
Achieving a spatial resolution that is not limited by the diffraction of light, recent developments of super-resolution fluorescence
Adaptive optical fluorescence microscopy.
Ji N. Nat Methods 2017 - Review. PMID 28362438
The past quarter century has witnessed rapid developments of fluorescence microscopy techniques that enable structural and functional imaging of biological specimens at unprecedented depth and resolution. ...Here I review recent work on incorporating adaptive optics, a technology originally applied in astronomical telescopes to combat atmospheric aberrations, to improve image quality of fluorescence microscopy for biological imaging....
The past quarter century has witnessed rapid developments of fluorescence microscopy techniques that enable structural and fun …
Fast Fluorescence Microscopy with Light Sheets.
Daetwyler S and Huisken J. Biol Bull 2016 - Review. PMID 27638692
In light sheet microscopy, optical sectioning by selective fluorescence excitation with a sheet of light is combined with fast full-frame acquisition. ...This review explains why light sheet microscopes are much faster and gentler than other established fluorescence microscopy techniques. ...
In light sheet microscopy, optical sectioning by selective fluorescence excitation with a sheet of light is combined with fast …
Fluorescence microscopy-a historical and technical perspective.
Renz M. Cytometry A 2013 - Review. PMID 23585290 Free article.
For a little more than a century, fluorescence microscopy has been an essential source of major discoveries in cell biology. Recent developments improved both visualization and quantification by fluorescence microscopy imaging and established a methodology of fluorescence microscopy. ...
For a little more than a century, fluorescence microscopy has been an essential source of major discoveries in cell biology. R …
Fluorescence microscopy.
Herman B. Curr Protoc Cell Biol 2002 - Review. PMID 18228404
Fluorescence microscopes are equipped to observe the fluorescence of one or more specific fluorophores to localize specific molecules and analyzed cellular structures. This unit discusses the optics and other components of the fluorescence microscope, their functions and adjustments, and the digital darkroom....
Fluorescence microscopes are equipped to observe the fluorescence of one or more specific fluorophores to localize specific mo
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