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Year Number of Results
1976 1
1997 3
1998 1
1999 1
2000 2
2001 4
2002 9
2003 357
2004 926
2005 898
2006 1006
2007 1034
2008 918
2009 907
2010 1015
2011 1014
2012 1085
2013 1104
2014 1052
2015 1004
2016 920
2017 850
2018 823
2019 394
2020 8
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13,507 results
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Page 1
Mouse APOBEC3 expression in NIH 3T3 cells mediates hypermutation of AKV murine leukemia virus.
Boi S, et al. Virology 2018. PMID 29605684 Free PMC article.
Many mA3 studies have used NIH 3T3 cells assuming that endogenous mA3 production was negligible. We developed a monoclonal antibody specific for mA3 that reveals detectable mA3 in NIH 3T3 cells and we demonstrate that AKV released from the cells undergoes G→A hypermutation. ...Single cell analyses revealed that the expression of mA3 in NIH 3T3 cells was limited to 20% of the cells, which likely accounted for the abnormal distribution of mutations. ...
Many mA3 studies have used NIH 3T3 cells assuming that endogenous mA3 production was negligible. We developed a monoclo …
5-Azacytidine specifically inhibits the NIH-3T3 PCD process induced by TNF-alpha and cycloheximide via affecting BCL-XL.
Wang Q, et al. J Cell Biochem 2018. PMID 28777484
In this study, we used 5-AC treatment to investigate whether DNA methylation was involved in regulation of programmed cell death (PCD) in mouse embryo fibroblast NIH-3T3 cells which could undergo PCD after treatment with TNF-α and cycloheximide (CHX). The results showed that the genomic DNA of NIH-3T3 cells was hypermethylated during PCD induced by TNF-α and CHX, and 5-AC might prevent this PCD process. ...
In this study, we used 5-AC treatment to investigate whether DNA methylation was involved in regulation of programmed cell death (PCD) in mo …
Methods for synchronizing cells at specific stages of the cell cycle.
Jackman J and O'Connor PM. Curr Protoc Cell Biol 2001. PMID 18228388
Exponentially growing cells are asynchronous with respect to the cell cycle stage. Detection of cell cycle-related events is improved by enriching the culture for cells at the stage during which the particular event occurs. Methods for synchronizing cells are provided here, including those based on morphological features of the cell (mitotic shake-off), cellular metabolism (thymidine inhibition, isoleucine depravation), and chemical inhibitors of cell progression in G1 (lovastatin), S (aphidicolin, mimosine), and G2/M (nocodazole). ...
Exponentially growing cells are asynchronous with respect to the cell cycle stage. Detection of cell cycle-related events is improved …
Estimation of Viscoelastic Properties of Cells Using Acoustic Tweezing Cytometry.
Yang C, et al. J Ultrasound Med 2016. PMID 27872412 Free article.
RESULTS: The mean maximum displacement of the microbubbles attached to NIH/3T3 fibroblasts was much greater than that for ATDC5 cells. ...The rigidity for ATDC5 cells was 331.46 ± 106.50 MPa, whereas that for NIH/3T3 fibroblasts was 117.92 ± 34.83 MPa. CONCLUSIONS: The Arg-Gly-Asp-integrin-cytoskeleton system of NIH/3T3 fibroblasts appears to be softer than that of ATDC5 cells. ...
RESULTS: The mean maximum displacement of the microbubbles attached to NIH/3T3 fibroblasts was much greater than that for ATDC …
High expression of the circadian gene mPer2 diminishes the radiosensitivity of NIH 3T3 cells.
Chang L, et al. Braz J Med Biol Res 2009. PMID 19787145 Free article.
NIH 3T3 cells were treated with 12-O-tetradecanoylphorbol-13-acetate (TPA) to induce endogenous mPer2 expression or transfected with pcDNA3.1(+)-mPer2 and irradiated with 60Co-gamma-rays, and then analyzed by several methods such as flow cytometry, colony formation assay, RT-PCR, and immunohistochemistry. ...Thus, high expression of the circadian gene mPer2 may reduce the radiosensitivity of NIH 3T3 cells. For this effect, mPer2 may directly or indirectly regulate the expressions of cell proliferation- and apoptosis-related genes and DNA repair-related genes....
NIH 3T3 cells were treated with 12-O-tetradecanoylphorbol-13-acetate (TPA) to induce endogenous mPer2 expression or tra
Hollow microcarriers for large-scale expansion of anchorage-dependent cells in a stirred bioreactor.
YekrangSafakar A, et al. Biotechnol Bioeng 2018. PMID 29578573
To produce and maintain a very large population of anchorage-dependent cells, a microcarrier-based stirred tank bioreactor is commonly used. ...As a proof of concept, we demonstrated the expansion of fibroblasts, NIH/3T3 and the expansion and cardiac differentiation of human induced pluripotent stem cells, along with detailed numerical analysis. ...
To produce and maintain a very large population of anchorage-dependent cells, a microcarrier-based stirred tank bioreactor is commonl …
The effect of well-characterized, very low-dose x-ray radiation on fibroblasts
Truong K, et al. PLoS One 2018. PMID 29300773 Free PMC article.
This system generates characteristic fluorescent x-rays to irradiate the cell culture with x-rays of well-defined energies and doses. 3T3 fibroblast cells were cultured in cups with Mylar® surfaces and were irradiated for one hour with characteristic iron (Fe) K x-ray radiation at a dose rate of approximately 550 μGy/hr. ...Irradiated cells demonstrated increased proliferation and protein production compared to control samples. Flow cytometry revealed that a higher percentage of irradiated cells were in the G0/G1 phase of the cell cycle compared to control counterparts, which is consistent with other low-dose studies. ...
This system generates characteristic fluorescent x-rays to irradiate the cell culture with x-rays of well-defined energies and doses. 3T3
Cisplatin treatment of NIH/3T3 cultures induces a form of autophagic death in polyploid cells.
Spano A, et al. Histol Histopathol 2008. PMID 18366010
The effects induced by different concentrations (50, 75, 100 microM) of the cytostatic drug cisplatin (cDDP) in NIH/3T3 cells were analyzed. ...More actively proliferating cells (2c-4c DNA content) die throughout canonical apoptosis, while polyploid cells prevailingly degenerate by mechanisms partly referable to autophagic cell death....
The effects induced by different concentrations (50, 75, 100 microM) of the cytostatic drug cisplatin (cDDP) in NIH/3T3 cel
Methanol fixed fibroblasts serve as feeder cells to maintain stem cells in the pluripotent state in vitro.
Ren Y, et al. Sci Rep 2018. PMID 29773904 Free PMC article.
Preparation of mouse embryonic fibroblast (MEF) feeder cells to maintain pluripotent stem cells (PSCs) is time consuming and involved in animal issues. ...Alternatively, the immortalized cell lines, for instance NIH3T3 cells, could also be fixed by methanol and used as feeder cells to maintain PSCs. ...
Preparation of mouse embryonic fibroblast (MEF) feeder cells to maintain pluripotent stem cells (PSCs) is time consuming and i …
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