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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1945 2
1946 4
1947 2
1948 1
1949 1
1950 2
1951 8
1952 4
1953 5
1954 5
1955 1
1956 6
1957 2
1958 2
1959 4
1960 10
1961 12
1962 24
1963 22
1964 37
1965 13
1967 2
1968 21
1969 4
1970 33
1971 294
1972 525
1973 554
1974 706
1975 579
1976 545
1977 624
1978 605
1979 770
1980 805
1981 939
1982 1104
1983 1254
1984 1741
1985 1810
1986 2200
1987 2463
1988 3014
1989 3100
1990 3167
1991 3119
1992 3279
1993 3898
1994 4335
1995 4647
1996 4744
1997 5040
1998 5326
1999 5701
2000 6383
2001 7583
2002 7951
2003 8912
2004 10293
2005 11053
2006 11091
2007 11215
2008 10912
2009 11047
2010 11103
2011 10294
2012 9605
2013 9068
2014 8327
2015 7272
2016 6294
2017 5080
2018 3911
2019 2015
2020 57
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220,724 results
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Page 1
Selection of fluorophore and quencher pairs for fluorescent nucleic acid hybridization probes.
Marras SA. Methods Mol Biol 2006 - Review. PMID 16785616
The use of fluorescent hybridization probes that generate a fluorescence signal only when they bind to their target enables real-time monitoring of nucleic acid amplification assays. ...Because fluorescent hybridization probes are available in a wide range of colors, multiple hybridization probes, each designed for the detection of a different nucleic acid sequence and each labeled with a differently colored fluorophore, can be added to the same nucleic acid amplification reaction, enabling the development of high-throughput multiplex assays. ...
The use of fluorescent hybridization probes that generate a fluorescence signal only when they bind to their target enables real-time …
Hybridization of Environmental Microbial Community Nucleic Acids by GeoChip.
Van Nostrand JD, et al. Methods Mol Biol 2016. PMID 26791504
Here, we briefly describe GeoChip design strategies (gene selection and probe design) and discuss minimum quantity and quality requirements for nucleic acids. We then provide detailed protocols for amplification, labeling, and hybridization of samples to the GeoChip....
Here, we briefly describe GeoChip design strategies (gene selection and probe design) and discuss minimum quantity and quality requirements …
Self-Assembled Pico-Liter Droplet Microarray for Ultrasensitive Nucleic Acid Quantification.
Yen TM, et al. ACS Nano 2015. PMID 26435065 Free article.
Nucleic acid detection and quantification technologies have made remarkable progress in recent years. Among existing platforms, hybridization-based assays have the advantages of being amplification free, low instrument cost, and high throughput, but are generally less sensitive compared to sequencing and PCR assays. ...The pico-liter droplet array device is realized with a micropatterned superhydrophobic black silicon surface that allows enrichment of nucleic acid samples by position-defined evaporation. ...
Nucleic acid detection and quantification technologies have made remarkable progress in recent years. Among existing platforms
Peptide nucleic acid pre-gel hybridization: an alternative to southern hybridization.
Perry-O'Keefe H, et al. Proc Natl Acad Sci U S A 1996. PMID 8962112 Free PMC article.
We have found that it is possible to use labeled peptide nucleic acid (PNA)-oligomers as probes in pre-gel hybridization experiments, as an alternative for Southern hybridization. ...The neutral backbone of PNA allows for hybridization at low ionic strength and imparts very low mobility to excess PNA. Detection of the bound PNA is possible by direct fluorescence detection with capillary electrophoresis, or the DNA/PNA hybrids can be blotted onto a membrane and detected with standard chemiluminescent techniques. ...
We have found that it is possible to use labeled peptide nucleic acid (PNA)-oligomers as probes in pre-gel hybridization
Choosing reporter-quencher pairs for efficient quenching through formation of intramolecular dimers
Johansson MK. Methods Mol Biol 2006 - Review. PMID 16785617
Nucleic acid detection/amplification methods, such as real-time polymerase chain reaction, use dual-labeled probes to measure the presence and copy number of specific genes or expressed messenger RNA. ...Fluorescence from the reporter is only released when the two dyes are physically separated via hybridization or nuclease activity. ...
Nucleic acid detection/amplification methods, such as real-time polymerase chain reaction, use dual-labeled probes to measure
Peptide nucleic acid: a versatile tool in genetic diagnostics and molecular biology.
Nielsen PE. Curr Opin Biotechnol 2001 - Review. PMID 11167067
During the past ten years, the DNA mimic peptide nucleic acid has inspired the development of a variety of hybridisation-based methods for detection, quantification, purification and characterisation of nucleic acids. Most of these methods have taken advantage of the very favourable DNA and RNA hybridisation properties of peptide nucleic acids combined with the unique properties and opportunities offered by peptide chemistry. ...
During the past ten years, the DNA mimic peptide nucleic acid has inspired the development of a variety of hybridisation-based …
220,724 results
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