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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1988 2
1989 43
1990 298
1991 847
1992 1362
1993 1692
1994 1606
1995 1710
1996 1977
1997 2063
1998 2305
1999 2413
2000 2193
2001 2100
2002 2431
2003 3529
2004 3932
2005 4513
2006 5058
2007 4351
2008 4153
2009 4106
2010 3929
2011 3959
2012 3700
2013 3113
2014 2758
2015 2660
2016 2550
2017 2442
2018 2157
2019 999
2020 16
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74,562 results
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Page 1
The polymerase chain reaction (PCR): general methods.
Waters DL and Shapter FM. Methods Mol Biol 2014. PMID 24243196
The polymerase chain reaction (PCR) converts very low quantities of DNA into very high quantities and is the foundation of many specialized techniques of molecular biology. ...
The polymerase chain reaction (PCR) converts very low quantities of DNA into very high quantities and is the foundation …
Polymerase chain reaction: basic protocol plus troubleshooting and optimization strategies.
Lorenz TC. J Vis Exp 2012. PMID 22664923 Free PMC article.
The development of the polymerase chain reaction (PCR) is one of those innovations that changed the course of molecular science with its impact spanning countless subdisciplines in biology. ...Automation and refinement of this technique progressed with the introduction of a thermal stable DNA polymerase from the bacterium Thermus aquaticus, consequently the name Taq DNA polymerase. ...
The development of the polymerase chain reaction (PCR) is one of those innovations that changed the course of molecular …
Principles and applications of polymerase chain reaction: basic science for the practicing physician.
Ishmael FT and Stellato C. Ann Allergy Asthma Immunol 2008. PMID 18939735
OBJECTIVE: To review the technology of polymerase chain reaction (PCR) and its use in diagnostic medicine and biomedical research. DATA SOURCES: A literature review was performed using the PubMed database for English-language articles published between January 1, 1985, and November 31, 2007, using the following keywords: polymerase chain reaction, real-time polymerase chain reaction, and applications of real-time polymerase chain reaction. ...
OBJECTIVE: To review the technology of polymerase chain reaction (PCR) and its use in diagnostic medicine and biomedica …
Advances in digital polymerase chain reaction (dPCR) and its emerging biomedical applications.
Cao L, et al. Biosens Bioelectron 2017 - Review. PMID 27818047
Since the invention of polymerase chain reaction (PCR) in 1985, PCR has played a significant role in molecular diagnostics for genetic diseases, pathogens, oncogenes and forensic identification. ...
Since the invention of polymerase chain reaction (PCR) in 1985, PCR has played a significant role in molecular diagnost …
The Basic Polymerase Chain Reaction (PCR).
Green MR and Sambrook J. Cold Spring Harb Protoc 2018. PMID 29717051
This protocol describes the reagents and procedures required to amplify a segment of double-stranded DNA in a chain reaction catalyzed by a thermostable DNA polymerase. It is the foundation for all subsequent variations of the polymerase chain reaction (PCR)....
This protocol describes the reagents and procedures required to amplify a segment of double-stranded DNA in a chain reaction c …
Reverse Transcription Polymerase Chain Reaction in Giant Unilamellar Vesicles.
Tsugane M and Suzuki H. Sci Rep 2018. PMID 29907779 Free PMC article.
We assessed the applicability of giant unilamellar vesicles (GUVs) for RNA detection using in vesicle reverse transcription polymerase chain reaction (RT-PCR). We prepared GUVs that encapsulated one-pot RT-PCR reaction mixture including template RNA, primers, and Taqman probe, using water-in-oil emulsion transfer method. ...
We assessed the applicability of giant unilamellar vesicles (GUVs) for RNA detection using in vesicle reverse transcription polymerase
Quantification of DNA by Real-Time Polymerase Chain Reaction (PCR).
Green MR and Sambrook J. Cold Spring Harb Protoc 2018. PMID 30275076
There are few differences between the experimental steps necessary for amplifying template DNA in a real-time thermocycler and a standard polymerase chain reaction (PCR). ...
There are few differences between the experimental steps necessary for amplifying template DNA in a real-time thermocycler and a standard …
Mathematics analysis of polymerase chain reaction kinetic curves.
Sochivko DG, et al. Dokl Biochem Biophys 2016. PMID 27025478
The paper reviews different approaches to the mathematical analysis of polymerase chain reaction (PCR) kinetic curves. The basic principles of PCR mathematical analysis are presented. ...
The paper reviews different approaches to the mathematical analysis of polymerase chain reaction (PCR) kinetic curves. …
Random mutagenesis by error-prone PCR
McCullum EO, et al. Methods Mol Biol 2010. PMID 20676978
Here, we describe a general method to introduce random nucleotide mutations into a parent sequence that takes advantage of the polymerase chain reaction (PCR). This protocol reduces mutational bias often associated with error-prone PCR methods and allows the experimenter to control the degree of mutagenesis by controlling the number of gene-doubling events that occur in the PCR reaction. ...
Here, we describe a general method to introduce random nucleotide mutations into a parent sequence that takes advantage of the polymerase
Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology
Smith CJ and Osborn AM. FEMS Microbiol Ecol 2009 - Review. PMID 19120456 Free article.
When Q-PCR is coupled with a preceding reverse transcription reaction, it can be used to quantify gene expression (RT-Q-PCR). ...
When Q-PCR is coupled with a preceding reverse transcription reaction, it can be used to quantify gene expression (RT-Q-PCR). ...
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