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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1986 4
1987 2
1988 2
1989 5
1990 13
1991 37
1992 44
1993 59
1994 61
1995 58
1996 46
1997 64
1998 93
1999 122
2000 120
2001 168
2002 238
2003 431
2004 555
2005 588
2006 612
2007 626
2008 581
2009 587
2010 576
2011 615
2012 616
2013 599
2014 540
2015 577
2016 616
2017 635
2018 603
2019 274
2020 1
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9,784 results
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Page 1
Protein Engineering Approaches in the Post-Genomic Era.
Singh RK, et al. Curr Protein Pept Sci 2018 - Review. PMID 27855603
Protein engineering aims to design new proteins or modify the sequence of a protein to create proteins with new or desirable functions. ...Rational design is an effective method of protein engineering when the threedimensional structure and mechanism of the protein is well known. ...
Protein engineering aims to design new proteins or modify the sequence of a protein to create proteins with new or desi
Protein Engineering: Past, Present, and Future.
Lutz S and Iamurri SM. Methods Mol Biol 2018 - Review. PMID 29086300
This trend has to a significant degree been fueled by advances in scientists' and engineers' ability to customize native enzymes by protein engineering. ...Herein, we use history to guide a brief overview of the major strategies for protein engineering-past, present, and future....
This trend has to a significant degree been fueled by advances in scientists' and engineers' ability to customize native enzymes
Applications of Yeast Surface Display for Protein Engineering.
Cherf GM and Cochran JR. Methods Mol Biol 2015 - Review. PMID 26060074 Free PMC article.
The method of displaying recombinant proteins on the surface of Saccharomyces cerevisiae via genetic fusion to an abundant cell wall protein, a technology known as yeast surface display, or simply, yeast display, has become a valuable protein engineering tool for a broad spectrum of biotechnology and biomedical applications. This review focuses on the use of yeast display for engineering protein affinity, stability, and enzymatic activity. Strategies and examples for each protein engineering goal are discussed. ...
The method of displaying recombinant proteins on the surface of Saccharomyces cerevisiae via genetic fusion to an abundant cell wall prot
Deep sequencing methods for protein engineering and design.
Wrenbeck EE, et al. Curr Opin Struct Biol 2017 - Review. PMID 27886568 Free PMC article.
The advent of next-generation sequencing (NGS) has revolutionized protein science, and the development of complementary methods enabling NGS-driven protein engineering have followed. ...Additionally, we highlight the generation of enzyme fitness landscapes for conducting fundamental studies of protein behavior and evolution. ...
The advent of next-generation sequencing (NGS) has revolutionized protein science, and the development of complementary methods en
Protein engineering and its applications in food industry.
Kapoor S, et al. Crit Rev Food Sci Nutr 2017 - Review. PMID 26065315
Protein produced properly from genetic engineering process means a protein that is able to fold correctly and to do particular function(s) efficiently even after being subjected to engineering practices. Protein is modified through its gene or chemically. However, modification of protein through gene is easier. There is no specific limitation of Protein Engineering tools; any technique that can lead to change the protein constituent of amino acid and result in the modification of protein structure/function is in the frame of Protein Engineering. ...
Protein produced properly from genetic engineering process means a protein that is able to fold correctly and to do par
Unified rational protein engineering with sequence-based deep representation learning.
Alley EC, et al. Nat Methods 2019. PMID 31636460
Rational protein engineering requires a holistic understanding of protein function. Here, we apply deep learning to unlabeled amino-acid sequences to distill the fundamental features of a protein into a statistical representation that is semantically rich and structurally, evolutionarily and biophysically grounded. ...UniRep further enables two orders of magnitude efficiency improvement in a protein engineering task. UniRep is a versatile summary of fundamental protein features that can be applied across protein engineering informatics....
Rational protein engineering requires a holistic understanding of protein function. Here, we apply deep learning to unl …
Membrane protein engineering to the rescue.
Rawlings AE. Biochem Soc Trans 2018 - Review. PMID 30381335 Free article.
For many years, the only way of working with membrane proteins was to optimise the environment to suit the protein, through the use of different detergents, solubilising additives, and other adaptations. However, with innovative protein engineering methodologies, the membrane proteins themselves are now being adapted to suit the environment. ...
For many years, the only way of working with membrane proteins was to optimise the environment to suit the protein, through th …
Protein engineering: the potential of remote mutations.
Wilding M, et al. Biochem Soc Trans 2019 - Review. PMID 30902926
This minireview will discuss the current state of the art in protein engineering, with a particular focus on engineering regions that are remote from active- or ligand-binding sites. As the use of protein technologies expands, exploiting the potential improvements made possible through modifying remote regions will become vital if we are to realise the full potential of protein engineering and design....
This minireview will discuss the current state of the art in protein engineering, with a particular focus on engineering
Protein disulfide engineering.
Dombkowski AA, et al. FEBS Lett 2014 - Review. PMID 24291258 Free article.
Disulfide bonds are covalent interactions that provide substantial stability to many proteins and conform to well-defined geometric conformations, thus making them appealing candidates in protein engineering efforts. ...Contrary to expectations, many designed disulfide bonds have resulted in decreased stability of the modified protein. We review progress in disulfide engineering, with an emphasis on the issue of stability and computational methods that facilitate engineering efforts....
Disulfide bonds are covalent interactions that provide substantial stability to many proteins and conform to well-defined geometric conforma …
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