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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1967 1
1973 1
1976 2
1986 1
1987 2
1988 2
1989 9
1990 12
1991 44
1992 86
1993 160
1994 243
1995 302
1996 326
1997 432
1998 522
1999 646
2000 633
2001 593
2002 554
2003 524
2004 553
2005 594
2006 532
2007 597
2008 549
2009 608
2010 625
2011 632
2012 560
2013 546
2014 523
2015 499
2016 479
2017 454
2018 353
2019 149
2020 0
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12,514 results
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Page 1
Microfluidic System for Detection of Viral RNA in Blood Using a Barcode Fluorescence Reporter and a Photocleavable Capture Probe.
Du K, et al. Anal Chem 2017. PMID 29073356 Free PMC article.
A microfluidic sample preparation multiplexer (SPM) and assay procedure is developed to improve amplification-free detection of Ebola virus RNA from blood. While a previous prototype successfully detected viral RNA following off-chip RNA extraction from infected cells, the new device and protocol can detect Ebola virus in raw blood with clinically relevant sensitivity. ...
A microfluidic sample preparation multiplexer (SPM) and assay procedure is developed to improve amplification-free detection of Ebola virus …
A paper-based platform for detection of viral RNA.
Zhang D, et al. Analyst 2017. PMID 28194453 Free PMC article.
Viral detection presents a host of challenges for even the most sensitive analytical techniques, and the complexity of common detection platforms typically preclude portability. ...The sensing system can detect viral cDNA reverse-transcribed from total RNA extraction by utilizing a biotinylated capture probe and an Alexa Fluor® 647-labeled reporter probe. ...
Viral detection presents a host of challenges for even the most sensitive analytical techniques, and the complexity of common detecti
Ebola Virus RNA Stability in Human Blood and Urine in West Africa's Environmental Conditions.
Janvier F, et al. Emerg Infect Dis 2016. PMID 26812135 Free PMC article.
We evaluated RNA stability of Ebola virus in EDTA blood and urine samples collected from infected patients and stored in West Africa's environmental conditions. In blood, RNA was stable for at least 18 days when initial cycle threshold values were <30, but in urine, RNA degradation occurred more quickly....
We evaluated RNA stability of Ebola virus in EDTA blood and urine samples collected from infected patients and stored in West …
Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood.
Fleming DS, et al. J Vis Exp 2018. PMID 30582594
This manuscript demonstrates a modified protocol for the generation mRNA and non-coding RNA expression libraries, including viral RNA, from a single sample of whole blood. Optimization of this protocol, improved RNA purity, increased ligation for recovery of methylated RNAs, and omitted size selection, to allow capture of more RNA species....
This manuscript demonstrates a modified protocol for the generation mRNA and non-coding RNA expression libraries, including viral
HTLV-1 viral RNA is detected rarely in plasma of HTLV-1 infected subjects.
Demontis MA, et al. J Med Virol 2015. PMID 25982784 Free article.
HTLV-1 RNA was detected in plasma from 18 (28%) patients, always at a very low copy number (3-13 copies viral cDNA per milliliter of plasma). Mean values of HTLV-1 proviral load did not differ between patients in whom HTLV-1 RNA was detected and patients in whom it was not possible to detect HTLV-1 RNA in plasma. ...
HTLV-1 RNA was detected in plasma from 18 (28%) patients, always at a very low copy number (3-13 copies viral cDNA per millili …
Distribution of hepatitis C virus (HCV) RNA in whole blood and blood cell fractions: plasma HCV RNA analysis underestimates circulating virus load.
Schmidt WN, et al. J Infect Dis 1997. PMID 9207345
Previous experiments using a cationic surfactant to detect hepatitis C virus (HCV) RNA in whole blood (WB) suggested that WB was a more plentiful source of viral RNA than was plasma. ...These data indicate that a significant proportion of HCV RNA in peripheral blood is not identified by standard plasma RNA detection methods....
Previous experiments using a cationic surfactant to detect hepatitis C virus (HCV) RNA in whole blood (WB) suggested that WB w …
A fully-integrated and automated testing device for PCR-free viral nucleic acid detection in whole blood.
Liu W, et al. Lab Chip 2018. PMID 29881833
Here, we present a fully integrated and simple-to-use POC device operated by a passive fluidic method that is able to perform a sequential multi-step assay to detect viral nucleic acids in blood. This simple device enabled the rapid detection of hepatitis C virus in blood in approximately 30 minutes with minimal sample handling by the user....
Here, we present a fully integrated and simple-to-use POC device operated by a passive fluidic method that is able to perform a sequential m …
Detection of hepatitis E virus RNA in saliva for diagnosis of acute infection.
Rivero-Juarez A, et al. Zoonoses Public Health 2018. PMID 29659194
Diagnosis of acute hepatitis E virus (HEV) infection is established by detection of anti-HEV IgM antibodies by ELISA or by amplification of serum viral RNA. ...Whole blood and neat saliva samples were obtained from all patients. Saliva samples were processed and analysed for HEV RNA by RT-PCR within 2 hr after collection. ...
Diagnosis of acute hepatitis E virus (HEV) infection is established by detection of anti-HEV IgM antibodies by ELISA or by amplification of …
The comparisons of hepatitis C virus RNA level between intraoperative blood salvage (cell saver) and systemic hepatitis C virus RNA kinetics during liver transplantation.
Ko JS, et al. Transplant Proc 2014. PMID 24935309
In this study, we compared HCV RNA levels of CS blood with a series of blood samples obtained from HCV-infected OLT recipients. METHODS: Twelve HCV-infected patients with >50,000 copies/mL of HCV RNA were enrolled. HCV RNA was measured immediately after induction (I), at the end of anhepatic period (II), at the end of operation (III), and from the first returned blood in CS (CSb). ...
In this study, we compared HCV RNA levels of CS blood with a series of blood samples obtained from HCV-infected OLT rec …
A Direct from Blood/Plasma Reverse Transcription-Polymerase Chain Reaction for Dengue Virus Detection in Point-of-Care Settings.
Mehta N, et al. Am J Trop Med Hyg 2019. PMID 30994095 Free PMC article.
Rapid diagnostic tests that are used presently for point-of-care detection of DENV antigens lack the sensitivity of molecular diagnostics that detect viral RNA. ...Reverse transcription-polymerase chain reaction was performed directly from plasma samples without RNA extraction. The assay detected all four serotypes of DENV spiked into blood or plasma. ...
Rapid diagnostic tests that are used presently for point-of-care detection of DENV antigens lack the sensitivity of molecular diagnostics th …
12,514 results
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