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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
2002 1
2003 2
2004 1
2005 5
2006 3
2007 7
2008 9
2009 30
2010 203
2011 4723
2012 10200
2013 10282
2014 9777
2015 9589
2016 8016
2017 6044
2018 4387
2019 2050
2020 49
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55,487 results
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Page 1
New Developments in Quantitative Real-time Polymerase Chain Reaction Technology.
Gadkar Vy and Filion M. Curr Issues Mol Biol 2014 - Review. PMID 23562919 Free article.
Real time-quantitative PCR (RT-qPCR) technology has revolutionized the detection landscape in every area of molecular biology. ...These improvements have ranged from changes in the individual components of the enzymatic reaction cocktail (polymerizing enzymes, reaction buffers, probes, etc.) to the detection system itself (instrumentation, software, etc.). ...
Real time-quantitative PCR (RT-qPCR) technology has revolutionized the detection landscape in every area of molecular biology.
Multiplex Analyses Using Real-Time Quantitative PCR.
Hawkins SF and Guest PC. Methods Mol Biol 2017 - Review. PMID 27896761
Quantitative polymerase chain reaction (qPCR) is a routinely used method for the detection and quantitation of gene expression in real time. ...Here, we describe a method using the SensiFAST and SensiFAST One-Step probe kits which allows simultaneous real-time quantitation of up to 5 amplicons....
Quantitative polymerase chain reaction (qPCR) is a routinely used method for the detection and quantitation of gene exp …
Quantification of DNA by Real-Time Polymerase Chain Reaction (PCR).
Green MR and Sambrook J. Cold Spring Harb Protoc 2018. PMID 30275076
There are few differences between the experimental steps necessary for amplifying template DNA in a real-time thermocycler and a standard polymerase chain reaction (PCR). In real-time PCR, it is necessary, however, to optimize the concentration of primers and probe and to perform a standard curve. ...
There are few differences between the experimental steps necessary for amplifying template DNA in a real-time thermocycler and …
Analysis and Normalization of Real-Time Polymerase Chain Reaction (PCR) Experimental Data.
Green MR and Sambrook J. Cold Spring Harb Protoc 2018. PMID 30275081
In real-time polymerase chain reaction (PCR), also called quantitative real-time PCR [or simply quantitative PCR (qPCR)] or kinetic PCR, the amplification of DNA is monitored by the detection and quantitation of a fluorescent reporter signal, which increases in direct proportion to the amount of PCR product in the reaction. ...Therefore, it is critical to optimize the reagents and reaction conditions, include proper internal and external controls, and perform rigorous data analysis in order to generate accurate and reproducible results in real-time PCR experiments....
In real-time polymerase chain reaction (PCR), also called quantitative real-time PCR [or si …
Constructing a Standard Curve for Real-Time Polymerase Chain Reaction (PCR) Experiments.
Green MR and Sambrook J. Cold Spring Harb Protoc 2018. PMID 30275075
It is essential to prepare a standard curve for every real-time polymerase chain reaction (PCR) experiment. This protocol is used to construct a standard curve in which the template concentration is unknown. ...
It is essential to prepare a standard curve for every real-time polymerase chain reaction (PCR) experimen …
Performance evaluation of optimal real-time polymerase chain reaction achieved with reduced voltage.
Hwang JS, et al. Biomed Eng Online 2018. PMID 30396352 Free PMC article.
BACKGROUND: Polymerase chain reaction (PCR) is used in nucleic acid tests of infectious diseases in point-of-care testing. Previous studies have demonstrated real-time PCR that uses a micro-PCR chip made of packing tape, double-sided tape, and a plastic cover with polycarbonate or polypropylene on a black matte printed circuit board substrate. ...The 2.5 V power test was more efficient for reducing the overall running time....
BACKGROUND: Polymerase chain reaction (PCR) is used in nucleic acid tests of infectious diseases in point-of-care testi …
PCR-Based Detection Methods for Single-Nucleotide Polymorphism or Mutation: Real-Time PCR and Its Substantial Contribution Toward Technological Refinement.
Matsuda K. Adv Clin Chem 2017 - Review. PMID 28431642
In this regard, polymerase chain reaction (PCR) has provided the necessary analytical performance for many molecular analyses. PCR-based methods for SNP/mutation detection are broadly categorized into two types-(1) polymorphic or mutant allele-directed specific analysis using primers matched with substituted nucleotide or using oligonucleotides to block or clamp the nontargeted template, and (2) melting curve analysis, which is combined with the real-time PCR techniques using hydrolysis probes, hybridization probes, or double-stranded DNA-binding fluorescent dyes. ...
In this regard, polymerase chain reaction (PCR) has provided the necessary analytical performance for many molecular an …
Advances in digital polymerase chain reaction (dPCR) and its emerging biomedical applications.
Cao L, et al. Biosens Bioelectron 2017 - Review. PMID 27818047
Since the invention of polymerase chain reaction (PCR) in 1985, PCR has played a significant role in molecular diagnostics for genetic diseases, pathogens, oncogenes and forensic identification. In the past three decades, PCR has evolved from end-point PCR, through real-time PCR, to its current version, which is the absolute quantitive digital PCR (dPCR). ...
Since the invention of polymerase chain reaction (PCR) in 1985, PCR has played a significant role in molecular diagnost …
Quantification of bovine leukemia virus proviral DNA using a low-cost real-time polymerase chain reaction.
Petersen MI, et al. J Dairy Sci 2018. PMID 29655562 Free article.
However, the high cost related to real-time PCR assays has limited their systematic inclusion in BLV surveillance and eradication programs. The aim of the present study was to validate a low-cost quantitative real-time PCR. Interestingly, by using SYBR Green detection dye, we were able to reduce the cost of a single reaction by a factor of 5 compared with most common assays based on the use of fluorogenic probes (i.e., TaqMan technology). ...
However, the high cost related to real-time PCR assays has limited their systematic inclusion in BLV surveillance and eradicat …
Real Time Polymerase Chain Reaction (rt-PCR): A New Patent to Diagnostic Purposes for Paracoccidioidomycosis.
Rocha-Silva F, et al. Recent Pat Endocr Metab Immune Drug Discov 2017. PMID 27593543
OBJECTIVE: The present study aimed to standardize the Real Time-Polymerase Chain Reaction (rt-PCR) for an efficient and safe PCM diagnosis amplifying the recombinant protein PB27 gene, only expressed by specimens of Paracoccidioides genus. ...CONCLUSION: The reaction developed was standardized and patented, opening perspectives to molecular diagnosis development for paracoccidioidomycosis, since rt-PCR can be applied to a broad spectrum of infectious diseases. ...
OBJECTIVE: The present study aimed to standardize the Real Time-Polymerase Chain Reaction (rt-PCR) for an …
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