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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1983 2
1984 1
1985 2
1986 33
1987 147
1988 153
1989 168
1990 176
1991 268
1992 661
1993 405
1994 548
1995 657
1996 990
1997 989
1998 1243
1999 1729
2000 1879
2001 1896
2002 2132
2003 1812
2004 1967
2005 1851
2006 1849
2007 1747
2008 1759
2009 2204
2010 2011
2011 1770
2012 1534
2013 1329
2014 1236
2015 1145
2016 992
2017 952
2018 696
2019 277
2020 0
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36,628 results
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Page 1
BioID: A Proximity-Dependent Labeling Approach in Proteomics Study
Li P, et al. Methods Mol Biol 2019. PMID 30276738
PDL requires the fusion expression of the target protein with an enzyme which catalyzes the attachment of a reactive molecule to the interacting partners in a distance-dependent manner. Further analysis of all the proteins that are modified by the reactive molecule discloses the identity of these proteins which are presumed to be interacting partners of the target protein. ...
PDL requires the fusion expression of the target protein with an enzyme which catalyzes the attachment of a reactive molecule to the …
Anticalin(®) Proteins as Therapeutic Agents in Human Diseases.
Rothe C and Skerra A. BioDrugs 2018 - Review. PMID 29748739 Free PMC article.
Their robust format allows for modification in several ways, both as fusion proteins and by chemical conjugation, for example, to tune plasma half-life. ...Moreover, Anticalin proteins allow molecular formatting as bi- and even multispecific fusion proteins, especially in combination with antibodies that provide a second specificity. ...
Their robust format allows for modification in several ways, both as fusion proteins and by chemical conjugation, for example, …
Synthetic fusion protein design and applications.
Yu K, et al. Biotechnol Adv 2015 - Review. PMID 25450191
Synthetic fusion proteins can be designed to achieve improved properties or new functionality by synergistically incorporating multiple proteins into one complex. ...Subsequently, the progress in structural prediction of fusion proteins is presented, which can potentially facilitate the structure-based systematic design of fusion proteins toward identifying the best combinations of fusion partners. ...
Synthetic fusion proteins can be designed to achieve improved properties or new functionality by synergistically incorporating …
Construction of Lasso Peptide Fusion Proteins.
Zong C, et al. ACS Chem Biol 2016. PMID 26492187 Free PMC article.
Here, we demonstrate fusion of two model proteins, the artificial leucine zipper A1 and the superfolder variant of GFP, to the C-terminus of the lasso peptide astexin-1. Successful lasso cyclization of the N-terminus of these fusion proteins requires a flexible linker in between the C-terminus of the lasso peptide and the N-terminus of the protein of interest. ...
Here, we demonstrate fusion of two model proteins, the artificial leucine zipper A1 and the superfolder variant of GFP, to the …
The promises and challenges of fusion constructs in protein biochemistry and enzymology.
Yang H, et al. Appl Microbiol Biotechnol 2016 - Review. PMID 27541749
Fusion constructs are used to improve the properties of or impart novel functionality to proteins for biotechnological applications. ...This review is expected to increase interest in and advance fusion strategies for optimization of enzymes and other functional proteins....
Fusion constructs are used to improve the properties of or impart novel functionality to proteins for biotechnological applica
CRISPR catch-up
[No authors listed] Nat Biotechnol 2017. PMID 28486443
Tagging Recombinant Proteins to Enhance Solubility and Aid Purification.
Loughran ST and Walls D. Methods Mol Biol 2017. PMID 27730552
Protein fusion technology has had a major impact on the efficient production and purification of individual recombinant proteins. ...In the case of large-scale proteomic studies, the development of purification procedures tailored to individual proteins is not practicable, and affinity tags have therefore become indispensable tools for structural and functional proteomic initiatives that involve the expression of many proteins in parallel. ...
Protein fusion technology has had a major impact on the efficient production and purification of individual recombinant pro
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