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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1982 7
1983 3
1984 3
1985 45
1986 208
1987 364
1988 534
1989 794
1990 1398
1991 1899
1992 3059
1993 4243
1994 4936
1995 6614
1996 6955
1997 6797
1998 6907
1999 7316
2000 8295
2001 7851
2002 7746
2003 7751
2004 7224
2005 6096
2006 6374
2007 6408
2008 6117
2009 6626
2010 6439
2011 6428
2012 6232
2013 5589
2014 5015
2015 5029
2016 4577
2017 4639
2018 3448
2019 1455
2020 22
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159,775 results
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Page 1
Strategies for optimization of heterologous protein expression in E. coli: Roadblocks and reinforcements
Kaur J, et al. Int J Biol Macromol 2018 - Review. PMID 28830778
E. coli is most preferred system used for the production of recombinant proteins in bacteria and the availability of improved genetic tools/methods are making it more valuable than ever. ...A number of vectors, their compatible hosts and culture conditions can be used to express recombinant proteins in large amounts and in native form. ...
E. coli is most preferred system used for the production of recombinant proteins in bacteria and the availability of improved …
Recombinant Protein Expression in Escherichia coli (E.coli): What We Need to Know.
Hayat SMG, et al. Curr Pharm Des 2018 - Review. PMID 29384059
BACKGROUND: Host, vector, and culture conditions (including cultivation media) are considered among the three main elements contributing to a successful production of recombinant proteins. Accordingly, one of the most common hosts to produce recombinant therapeutic proteins is Escherichia coli. METHODOLOGY: A comprehensive literature review was performed to identify important factors affecting production of recombinant proteins in Escherichia coli. ...
BACKGROUND: Host, vector, and culture conditions (including cultivation media) are considered among the three main elements contributing to …
Developing Gram-negative bacteria for the secretion of heterologous proteins.
Burdette LA, et al. Microb Cell Fact 2018 - Review. PMID 30572895 Free PMC article.
Gram-negative bacteria are attractive hosts for recombinant protein production because they are fast growing, easy to manipulate, and genetically stable in large cultures. ...Thus, researchers are devoting significant attention to engineering Gram-negative bacteria to secrete recombinant proteins to the extracellular medium. ...
Gram-negative bacteria are attractive hosts for recombinant protein production because they are fast growing, easy to manipulate, and …
Development of Recombinant Proteins to Treat Chronic Pain.
Prado J, et al. J Vis Exp 2018. PMID 29708532 Free PMC article.
Here, we describe the methods for production and quality control of IL4-10 recombinant fusion protein and we test the effectiveness of the IL4-10 fusion protein to resolve pain in a mouse model of persistent inflammatory pain....
Here, we describe the methods for production and quality control of IL4-10 recombinant fusion protein and we test the effectiveness o …
Continuous Manufacturing of Recombinant Therapeutic Proteins: Upstream and Downstream Technologies.
Patil R and Walther J. Adv Biochem Eng Biotechnol 2018 - Review. PMID 28265699
Continuous biomanufacturing of recombinant therapeutic proteins offers several potential advantages over conventional batch processing, including reduced cost of goods, more flexible and responsive manufacturing facilities, and improved and consistent product quality. ...
Continuous biomanufacturing of recombinant therapeutic proteins offers several potential advantages over conventional batch pr …
Poly-3-Hydroxybutyrate Functionalization with BioF-Tagged Recombinant Proteins.
Bello-Gil D, et al. Appl Environ Microbiol 2018. PMID 29196289 Free PMC article.
Here, the PHA-binding domain of the Pseudomonas putida KT2440 PhaF phasin (BioF polypeptide) was investigated as an affinity tag for the in vitro functionalization of poly-3-hydroxybutyrate (PHB) particles with recombinant proteins, namely, full-length PhaF and two fusion proteins tagged to BioF (BioF-C-LytA and BioF-β-galactosidase, containing the choline-binding module C-LytA and the β-galactosidase enzyme, respectively). ...Our results demonstrate the potentialities of PHA and the BioF tag for the construction of novel bioactive materials.IMPORTANCE Our results confirm the biotechnological potential of the BioF affinity tag as a versatile tool for functionalizing PHA supports with recombinant proteins, leading to novel bioactive materials. ...
Here, the PHA-binding domain of the Pseudomonas putida KT2440 PhaF phasin (BioF polypeptide) was investigated as an affinity tag for the in …
Transient Expression of Recombinant Membrane-eGFP Fusion Proteins in HEK293 Cells.
Pieprzyk J, et al. Methods Mol Biol 2018. PMID 30242677
Membrane proteins play important roles in many biological processes and are a major drug target. However, only a limited number of structures of eukaryotic membrane proteins have been determined so far. Besides the challenges in crystallizing these proteins, one of the main bottlenecks in structure determination is their recombinant expression. ...
Membrane proteins play important roles in many biological processes and are a major drug target. However, only a limited number of st …
Optimization of Protein Expression in Mammalian Cells
Hunter M, et al. Curr Protoc Protein Sci 2019 - Review. PMID 30265450
In both the industrial and academic environments, the use of recombinant proteins varies widely and, with it, the method of production. ...Here, we elaborate on the various obstacles encountered when planning high-yield experiments to produce the recombinant proteins of interest. © 2018 by John Wiley & Sons, Inc....
In both the industrial and academic environments, the use of recombinant proteins varies widely and, with it, the method of pr …
Perfusion mammalian cell culture for recombinant protein manufacturing - A critical review.
Bielser JM, et al. Biotechnol Adv 2018 - Review. PMID 29738813 Free article.
The manufacturing of recombinant protein is traditionally divided in two main steps: upstream (cell culture and synthesis of the target protein) and downstream (purification and formulation of the protein into a drug substance or drug product). ...
The manufacturing of recombinant protein is traditionally divided in two main steps: upstream (cell culture and synthesis of the targ …
Avian Bioreactor Systems: A Review
Woodfint RM, et al. Mol Biotechnol 2018 - Review. PMID 30306403
Animal bioreactors are genetically modified animal systems that have the potential to reduce production cost, and improve production efficiency, of pharmaceutically relevant recombinant proteins. ...Furthermore, recombinant proteins can be incorporated into egg whites and easily collected from the sterile environment of the egg. ...
Animal bioreactors are genetically modified animal systems that have the potential to reduce production cost, and improve production efficie …
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