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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1994 3
1995 5
1996 13
1997 68
1998 1609
1999 5028
2000 6023
2001 6801
2002 7153
2003 8809
2004 10226
2005 11395
2006 11546
2007 11556
2008 12043
2009 12576
2010 14818
2011 11769
2012 7943
2013 7882
2014 6896
2015 5657
2016 3804
2017 2426
2018 1225
2019 464
2020 12
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151,030 results
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Page 1
Quantification of mRNA using real-time RT-PCR
Nolan T, et al. Nat Protoc 2006. PMID 17406449
The real-time reverse transcription polymerase chain reaction (RT-qPCR) addresses the evident requirement for quantitative data analysis in molecular medicine, biotechnology, microbiology and diagnostics and has become the method of choice for the quantification of mRNA. ...We would like to emphasize, however, that RT-qPCR data constitute only a snapshot of information regarding the quantity of a given transcript in a cell or tissue. ...
The real-time reverse transcription polymerase chain reaction (RT-qPCR) addresses the evident requirement …
Quantification of RNA by Real-Time Reverse Transcription-Polymerase Chain Reaction (RT-PCR).
Green MR and Sambrook J. Cold Spring Harb Protoc 2018. PMID 30275077
This protocol describes a real-time reverse transcription-polymerase chain reaction (RT-PCR) assay using a two-enzyme, two-tube approach, carried out using either SYBR Green I or TaqMan chemistries. ...
This protocol describes a real-time reverse transcription-polymerase chain reaction (RT-PCR) assay using …
Clarifying Indeterminate Results on the Rabies Direct Fluorescent Antibody Test Using Real-Time Reverse Transcriptase Polymerase Chain Reaction.
Appler K, et al. Public Health Rep 2019. PMID 30508492 Free PMC article.
The objective of this study was to evaluate the number of unsatisfactory samples or samples with indeterminate results that were positive for rabies virus after additional testing using real-time reverse transcriptase polymerase chain reaction (RT-PCR). ...
The objective of this study was to evaluate the number of unsatisfactory samples or samples with indeterminate results that were positive fo …
Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays.
Bustin SA. J Mol Endocrinol 2000 - Review. PMID 11013345
The reverse transcription polymerase chain reaction (RT-PCR) is the most sensitive method for the detection of low-abundance mRNA, often obtained from limited tissue samples. ...It illustrates the usefulness of these assays by demonstrating the significantly different levels of transcription between individuals of the housekeeping gene family, glyceraldehyde-3-phosphate-dehydrogenase (GAPDH)....
The reverse transcription polymerase chain reaction (RT-PCR) is the most sensitive method for the detecti …
Reference gene selection for reverse transcription quantitative polymerase chain reaction in chicken hypothalamus under different feeding status.
Simon Á, et al. J Anim Physiol Anim Nutr (Berl) 2018. PMID 28294410
This study was designed to investigate the stability of 10 candidate reference genes, namely ACTB, B2M, GAPDH, HMBS, LBR, POLR2B, RN18S, RPS17, TBP, and YWHAZ for the normalization of gene expression data obtained by quantitative real-time polymerase chain reaction (qPCR) in studies related to feed intake of chicken. ...
This study was designed to investigate the stability of 10 candidate reference genes, namely ACTB, B2M, GAPDH, HMBS, LBR, POLR2B, RN18S, RPS …
Fabrication of 3D continuous-flow reverse-transcription polymerase chain reaction microdevice integrated with on-chip fluorescence detection for semi-quantitative assessment of gene expression.
Pham QN, et al. Analyst 2018. PMID 30318528
We fabricate a three-dimensional (3D) microdevice operated with minimal peripheral accessories, including a portable pump for semi-automated sample delivery and a single heater for temperature control, for performing reverse transcription polymerase chain reaction (RT-PCR) integrated with a downstream fluorescence detection module for semi-quantitative assessment of gene expression. The microdevice was fabricated by wrapping a polytetrafluoroethylene (PTFE) tube around a pre-designed polycarbonate mold to create a seamless microchannel for both the reverse transcription (RT) of RNA and the amplification of complementary DNA. ...
We fabricate a three-dimensional (3D) microdevice operated with minimal peripheral accessories, including a portable pump for semi-automated …
Statistical Analysis of Quantitative RT-PCR Results
Khan-Malek R and Wang Y. Methods Mol Biol 2017. PMID 28748470
Quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) represents a benchmark technology in the detection and quantification of mRNA. ...
Quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) represents a benchmark te …
Development and validation of a reverse transcription quantitative polymerase chain reaction for tilapia lake virus detection in clinical samples and experimentally challenged fish.
Tattiyapong P, et al. J Fish Dis 2018. PMID 29027697
In this study, a SYBR green-based reverse transcription quantitative polymerase chain reaction (RT-qPCR) assay targeting segment three of the virus was developed to detect and quantify TiLV in clinical samples and experimentally challenged fish. ...
In this study, a SYBR green-based reverse transcription quantitative polymerase chain reaction (RT-qPCR) …
Evaluation of candidate control genes for diagnosis and residual disease detection in leukemic patients using 'real-time' quantitative reverse-transcriptase polymerase chain reaction (RQ-PCR) - a Europe against cancer program.
Beillard E, et al. Leukemia 2003 - Clinical Trial. PMID 14562124
In order to correct variations in RNA quality and quantity and to calculate the sensitivity of each measurement, a control gene (CG) transcript should be amplified in parallel to the FG transcript. ...Overall, these data are not only eligible for quantification of fusion gene transcripts, but also for the quantification of aberrantly expressed genes....
In order to correct variations in RNA quality and quantity and to calculate the sensitivity of each measurement, a control gene (CG) tran
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