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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1996 2
1997 9
1998 171
1999 430
2000 322
2001 12
2002 202
2003 795
2004 1282
2005 1664
2006 1906
2007 1335
2008 1071
2009 993
2010 836
2011 672
2012 453
2013 367
2014 306
2015 262
2016 216
2017 200
2018 178
2019 86
2020 0
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12,391 results
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Quantification of mRNA using real-time RT-PCR
Nolan T, et al. Nat Protoc 2006. PMID 17406449
The real-time reverse transcription polymerase chain reaction (RT-qPCR) addresses the evident requirement for quantitative data analysis in molecular medicine, biotechnology, microbiology and diagnostics and has become the method of choice for the quantification of mRNA. ...We would like to emphasize, however, that RT-qPCR data constitute only a snapshot of information regarding the quantity of a given transcript in a cell or tissue. ...
The real-time reverse transcription polymerase chain reaction (RT-qPCR) addresses the evident requirement …
Real-time polymerase chain reaction and melting curve analysis.
Pryor RJ and Wittwer CT. Methods Mol Biol 2006. PMID 16916250
Monitoring polymerase chain reaction (PCR) once each cycle is a powerful method to detect and quantify the presence of nucleic acid sequences and has become known as "real-time" PCR. ...When used together with rapid temperature control, these methods allow amplification and genotyping in less than a half hour....
Monitoring polymerase chain reaction (PCR) once each cycle is a powerful method to detect and quantify the presence of …
Quantification of RNA by Real-Time Reverse Transcription-Polymerase Chain Reaction (RT-PCR).
Green MR and Sambrook J. Cold Spring Harb Protoc 2018. PMID 30275077
This protocol describes a real-time reverse transcription-polymerase chain reaction (RT-PCR) assay using a two-enzyme, two-tube approach, carried out using either SYBR Green I or TaqMan chemistries. ...
This protocol describes a real-time reverse transcription-polymerase chain reaction (RT-PCR) assay using …
Principles and applications of polymerase chain reaction: basic science for the practicing physician.
Ishmael FT and Stellato C. Ann Allergy Asthma Immunol 2008. PMID 18939735
OBJECTIVE: To review the technology of polymerase chain reaction (PCR) and its use in diagnostic medicine and biomedical research. DATA SOURCES: A literature review was performed using the PubMed database for English-language articles published between January 1, 1985, and November 31, 2007, using the following keywords: polymerase chain reaction, real-time polymerase chain reaction, and applications of real-time polymerase chain reaction. ...
OBJECTIVE: To review the technology of polymerase chain reaction (PCR) and its use in diagnostic medicine and biomedica …
Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology
Smith CJ and Osborn AM. FEMS Microbiol Ecol 2009 - Review. PMID 19120456 Free article.
By detection of amplicons during the early exponential phase of the PCR, this enables the quantification of gene (or transcript) numbers when these are proportional to the starting template concentration. When Q-PCR is coupled with a preceding reverse transcription reaction, it can be used to quantify gene expression (RT-Q-PCR). ...
By detection of amplicons during the early exponential phase of the PCR, this enables the quantification of gene (or transcript) numb …
Mapping Plastid Transcript Population by Circular Reverse Transcription Polymerase Chain Reaction.
Courtois F and Merendino L. Methods Mol Biol 2018. PMID 29987728
In addition, polycistronic transcripts undergo multiple processing events, thus generating a complex population of mRNAs from a single gene. ...
In addition, polycistronic transcripts undergo multiple processing events, thus generating a complex population of mRNAs from a singl …
Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction.
Bustin SA and Nolan T. J Biomol Tech 2004 - Review. PMID 15331581 Free PMC article.
Polymerase chain reaction (PCR)-based assays can target either DNA (the genome) or RNA (the transcriptome). Targeting the genome generates robust data that are informative and, most importantly, generally applicable. ...This makes the information contained within the transcriptome intrinsically flexible and variable. If this variability is combined with the technical limitations inherent in any reverse-transcription (RT)-PCR assay, it can be difficult to achieve not just a technically accurate but a biologically relevant result. ...
Polymerase chain reaction (PCR)-based assays can target either DNA (the genome) or RNA (the transcriptome). Targ
Reference gene selection for reverse transcription quantitative polymerase chain reaction in chicken hypothalamus under different feeding status.
Simon Á, et al. J Anim Physiol Anim Nutr (Berl) 2018. PMID 28294410
This study was designed to investigate the stability of 10 candidate reference genes, namely ACTB, B2M, GAPDH, HMBS, LBR, POLR2B, RN18S, RPS17, TBP, and YWHAZ for the normalization of gene expression data obtained by quantitative real-time polymerase chain reaction (qPCR) in studies related to feed intake of chicken. ...
This study was designed to investigate the stability of 10 candidate reference genes, namely ACTB, B2M, GAPDH, HMBS, LBR, POLR2B, RN18S, RPS …
Stem-loop RT-qPCR for miRNAs
Kramer MF. Curr Protoc Mol Biol 2011. PMID 21732315 Free PMC article.
This unit presents a specific and sensitive quantitative reverse-transcription PCR (RT-qPCR) method for measuring individual microRNAs (miRNAs) in tissue or cultured cells. miRNAs are 17 to 24 nucleotides (nt) in length. Standard and quantitative PCR methods require a template that is at least two times the length of either of the specific forward or reverse primers, each typically ∼ 20 nt in length. ...
This unit presents a specific and sensitive quantitative reverse-transcription PCR (RT-qPCR) method for measuring individual m …
Statistical Analysis of Quantitative RT-PCR Results
Khan-Malek R and Wang Y. Methods Mol Biol 2017. PMID 28748470
Quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) represents a benchmark technology in the detection and quantification of mRNA. ...Examples are used to illustrate the methods....
Quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) represents a benchmark te …
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