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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1954 1
1955 1
1961 1
1962 1
1965 2
1966 9
1967 61
1968 144
1969 177
1970 164
1971 216
1972 266
1973 265
1974 286
1975 257
1976 221
1977 200
1978 181
1979 200
1980 159
1981 126
1982 119
1983 139
1984 149
1985 133
1986 112
1987 100
1988 110
1989 146
1990 135
1991 146
1992 120
1993 132
1994 139
1995 121
1996 144
1997 194
1998 210
1999 283
2000 384
2001 625
2002 708
2003 778
2004 741
2005 787
2006 843
2007 864
2008 872
2009 870
2010 773
2011 747
2012 711
2013 764
2014 700
2015 692
2016 586
2017 516
2018 432
2019 152
2020 2
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17,799 results
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Page 1
Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous systems
Chan KY, et al. Nat Neurosci 2017. PMID 28671695 Free PMC article.
Nevertheless, AAVs that provide efficient transduction across specific organs or cell populations are needed. Here, we describe AAV-PHP.eB and AAV-PHP.S, capsids that efficiently transduce the central and peripheral nervous systems, respectively. ...Furthermore, when used with cell-type-specific promoters and enhancers, these AAVs enable efficient and targetable genetic modification of cells throughout the nervous system of transgenic and non-transgenic animals....
Nevertheless, AAVs that provide efficient transduction across specific organs or cell populations are needed. Here, we describe AAV-P …
Lentiviral transduction of mammalian cells for fast, scalable and high-level production of soluble and membrane proteins.
Elegheert J, et al. Nat Protoc 2018. PMID 30455477 Free PMC article.
Lentiviral transduction is an efficient method for the delivery of transgenes to mammalian cells and unifies the ease of use and speed of transient transfection with the robust expression of stable cell lines. ...We further compare this method with baculovirus transduction of mammalian cells (BacMam), using the type-A γ-aminobutyric acid receptor (GABA(A)R) β3 homopentamer as a test case. ...
Lentiviral transduction is an efficient method for the delivery of transgenes to mammalian cells and unifies the ease of use and spee …
Pseudotyped Lentiviral Vectors: One Vector, Many Guises
Joglekar AV and Sandoval S. Hum Gene Ther Methods 2017 - Review. PMID 28870117
Pseudotyping LVs with a diverse set of naturally occurring or engineered viral envelopes has allowed targeted transduction of specific cell types. ...These studies will expand the toolbox to make LVs that cater to the specific requirements of transduction. This review provides a comprehensive overview of various viral envelope pseudotypes used with LVs, their specificities, advantages, and drawbacks....
Pseudotyping LVs with a diverse set of naturally occurring or engineered viral envelopes has allowed targeted transduction of specifi …
Lentivirus-Based Stable Gene Delivery into Intestinal Organoids
Maru Y, et al. Methods Mol Biol 2016. PMID 27246018
By contrast, intestinal epithelial organoids embedded into three-dimensional extracellular matrix appear to be resistant to lentiviral transduction. ...In this chapter, we describe a simple method of a highly efficient gene transduction into intestinal organoids. This method involves organoid dispersion into single intestinal epithelial cells, mixing these individual cells with lentiviral particles, plating on Matrigel, and subsequent re-embedding into Matrigel. ...
By contrast, intestinal epithelial organoids embedded into three-dimensional extracellular matrix appear to be resistant to lentiviral tr
Efficient intracellular delivery of native proteins
D'Astolfo DS, et al. Cell 2015. PMID 25910214 Free article.
We developed a method termed iTOP, for induced transduction by osmocytosis and propanebetaine, in which a combination of NaCl hypertonicity-induced macropinocytosis and a transduction compound (propanebetaine) induces the highly efficient transduction of proteins into a wide variety of primary cells. ...
We developed a method termed iTOP, for induced transduction by osmocytosis and propanebetaine, in which a combination of NaCl hyperto …
Natural competence for transformation
Blokesch M. Curr Biol 2016. PMID 27825443 Free article.
While most molecular biologists are familiar with the artificial transformation of bacteria in the context of laboratory cloning experiments, natural competence for transformation refers to a specific physiological state in which prokaryotes are able to take up genetic material from their surroundings. ...As a consequence, natural competence for transformation is considered a primary mode of horizontal gene transfer (HGT) in prokaryotes, together with conjugation (direct cell to cell transfer of DNA via a specialized conjugal pilus) and phage transduction (DNA transfer mediated by viruses). ...
While most molecular biologists are familiar with the artificial transformation of bacteria in the context of laboratory cloning experiments …
CRISPR Screening in Single Cells
Henriksson J. Methods Mol Biol 2019. PMID 31028650
Practical Considerations for Using Pooled Lentiviral CRISPR Libraries
McDade JR, et al. Curr Protoc Mol Biol 2016 - Review. PMID 27366891
Several pooled lentiviral CRISPR libraries have been developed and are now publicly available, but while using CRISPR/Cas9 for genetic experiments has become widely adopted, genome-wide screening experiments remain technically challenging. ...
Several pooled lentiviral CRISPR libraries have been developed and are now publicly available, but while using CRISPR/Cas9 for genetic
Stable Expression by Lentiviral Transduction of Cells.
Gödecke N, et al. Methods Mol Biol 2018. PMID 30242679
Lentiviral gene transfer represents a versatile and powerful method for genetic transduction of many cell lines and primary cells including "hard-to-transfect" cells. ...
Lentiviral gene transfer represents a versatile and powerful method for genetic transduction of many cell lines and primary ce …
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