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Desiccation of Hypsibius exemplaris.
Boothby TC. Boothby TC. Cold Spring Harb Protoc. 2018 Nov 1;2018(11). doi: 10.1101/pdb.prot102327. Cold Spring Harb Protoc. 2018. PMID: 30385670
Many species of tardigrades can survive severe water loss, but different species tolerate different desiccation conditions. Hypsibius exemplaris is able to survive desiccation after an initial period of slow drying, as described here. ...
Many species of tardigrades can survive severe water loss, but different species tolerate different desiccation conditions. Hypsibius …
The Emergence of the Tardigrade Hypsibius exemplaris as a Model System.
Goldstein B. Goldstein B. Cold Spring Harb Protoc. 2018 Nov 1;2018(11). doi: 10.1101/pdb.emo102301. Cold Spring Harb Protoc. 2018. PMID: 30385668 Review.
Tardigrades, also known as water bears, make up a phylum of microscopic animals. The tardigrade Hypsibius exemplaris (recently disambiguated from a closely related species, Hypsibius dujardini) can be maintained in laboratories and has a generation tim …
Tardigrades, also known as water bears, make up a phylum of microscopic animals. The tardigrade Hypsibius exemplaris (recently …
Laboratory Culture of Hypsibius exemplaris.
McNuff R. McNuff R. Cold Spring Harb Protoc. 2018 Nov 1;2018(11). doi: 10.1101/pdb.prot102319. Cold Spring Harb Protoc. 2018. PMID: 30385669
I have reared a culture of the tardigrade Hypsibius exemplaris for 30 years, since 1987. Here, I present my culture protocol....
I have reared a culture of the tardigrade Hypsibius exemplaris for 30 years, since 1987. Here, I present my culture protocol.. …
Verification of Hypsibius exemplaris Gasiorek et al., 2018 (Eutardigrada; Hypsibiidae) application in anhydrobiosis research.
Poprawa I, Bartylak T, Kulpla A, Erdmann W, Roszkowska M, Chajec Ł, Kaczmarek Ł, Karachitos A, Kmita H. Poprawa I, et al. PLoS One. 2022 Mar 18;17(3):e0261485. doi: 10.1371/journal.pone.0261485. eCollection 2022. PLoS One. 2022. PMID: 35303010 Free PMC article.
The best known anhydrobiotic invertebrates are tardigrades (Tardigrada), considered to be toughest animals in the world. Hypsibius. exemplaris is one of the best studied tardigrade species, with its name "exemplaris" referring to the widespread use of the species as …
The best known anhydrobiotic invertebrates are tardigrades (Tardigrada), considered to be toughest animals in the world. Hypsibius. …
Uncommon N-Glycan Structures in Anhydrobiotic Tardigrades.
Yagi H, Saito T, Guu SY, Yamakawa N, Shimamura S, Kondo S, Yagi-Utsumi M, Takai K, Furukawa JI, Guérardel Y, Khoo KH, Arakawa K, Kato K. Yagi H, et al. Mol Cell Proteomics. 2025 Jun;24(6):100979. doi: 10.1016/j.mcpro.2025.100979. Epub 2025 Apr 28. Mol Cell Proteomics. 2025. PMID: 40306492 Free PMC article.
We characterized the N-glycosylation profiles of anhydrobiotic tardigrades, Ramazzottius varieornatus and Hypsibius exemplaris, identifying high-mannose, paucimannose, and complex-type oligosaccharides, while hybrid-type glycans were undetectable. ...
We characterized the N-glycosylation profiles of anhydrobiotic tardigrades, Ramazzottius varieornatus and Hypsibius exemplaris …
Microinjection of dsRNA in Tardigrades.
Tenlen JR. Tenlen JR. Cold Spring Harb Protoc. 2018 Nov 1;2018(11):10.1101/pdb.prot102368. doi: 10.1101/pdb.prot102368. Cold Spring Harb Protoc. 2018. PMID: 30385674 Free PMC article.
Classical genetic analysis in the tardigrade Hypsibius exemplaris is a challenge because these animals are parthenogens. The publication of the H. exemplaris genome has facilitated the study of targeted genes by RNA interference (RNAi), a robust mechanism to disrupt …
Classical genetic analysis in the tardigrade Hypsibius exemplaris is a challenge because these animals are parthenogens. The p …
Ecdysteroid-dependent molting in tardigrades.
Yamakawa S, Hejnol A. Yamakawa S, et al. Curr Biol. 2024 Dec 16;34(24):5804-5812.e4. doi: 10.1016/j.cub.2024.10.054. Epub 2024 Nov 19. Curr Biol. 2024. PMID: 39566498 Free article.
For example, involvement of the Ecd hormone in molting regulation has been suggested only in some parasitic nematodes outside of arthropods,(6)(,)(7) and insect Ecd synthesis and receptor genes are lacking in some ecysozoan lineages (Figure S1A).(8)(,)(9)(,)(10) In this study, we …
For example, involvement of the Ecd hormone in molting regulation has been suggested only in some parasitic nematodes outside of arthropods, …
Fluorescence shadow imaging of Hypsibius exemplaris reveals morphological differences between sucrose- and CaCl(2)-induced osmobiotes.
Flinn BB, O'Dell HM, Joseph KM, Smythers AL, Neff DP, Hicks LM, Norton ML, Kolling DRJ. Flinn BB, et al. Sci Rep. 2024 May 23;14(1):11845. doi: 10.1038/s41598-024-61374-y. Sci Rep. 2024. PMID: 38782941 Free PMC article.
Herein, an approach for discriminating between tardigrade morphological states is developed and utilized to compare sucrose- and CaCl(2)-induced tuns, using the model species Hypsibius exemplaris. A novel approach of shadow imaging with confocal laser scanning micro …
Herein, an approach for discriminating between tardigrade morphological states is developed and utilized to compare sucrose- and CaCl(2)-ind …
Carbonylation accumulation of the Hypsibius exemplaris anhydrobiote reveals age-associated marks.
Kuzmic M, Richaud M, Cuq P, Frelon S, Galas S. Kuzmic M, et al. PLoS One. 2018 Dec 26;13(12):e0208617. doi: 10.1371/journal.pone.0208617. eCollection 2018. PLoS One. 2018. PMID: 30586374 Free PMC article.
In this report, we show that the tardigrade Hypsibius exemplaris can accumulate molecular damages by means of in situ detection of carbonyls. ...
In this report, we show that the tardigrade Hypsibius exemplaris can accumulate molecular damages by means of in situ detectio …
Fluorescent Cell Staining Methods for Living Hypsibius exemplaris Embryos.
McGreevy KM, Heikes KL, Kult S, Tharp ME, Goldstein B. McGreevy KM, et al. Cold Spring Harb Protoc. 2018 Nov 1;2018(11). doi: 10.1101/pdb.prot106021. Cold Spring Harb Protoc. 2018. PMID: 30385676
The tardigrade Hypsibius exemplaris was chosen as a model system in part because animals and embryos are optically clear at all stages, facilitating the visualization of events in living material. ...
The tardigrade Hypsibius exemplaris was chosen as a model system in part because animals and embryos are optically clear at al …
112 results