Ablation of SYK Kinase from Expanded Primary Human NK Cells via CRISPR/Cas9 Enhances Cytotoxicity and Cytokine Production.
Dahlvang JD, Dick JK, Sangala JA, Kennedy PR, Pomeroy EJ, Snyder KM, Moushon JM, Thefaine CE, Wu J, Hamilton SE, Felices M, Miller JS, Walcheck B, Webber BR, Moriarity BS, Hart GT.
Dahlvang JD, et al.
J Immunol. 2023 Apr 15;210(8):1108-1122. doi: 10.4049/jimmunol.2200488.
J Immunol. 2023.
PMID: 36881874
Free PMC article.
We ablated genes that encode molecules in the ADCC pathway, such as FcRgamma, CD3zeta, SYK, SHP-1, ZAP70, and the transcription factor PLZF, and tested subsequent ADCC and cytokine production. ...Importantly, SYK kinase ablation significantly enhanced cytotoxicity, cytokin …
We ablated genes that encode molecules in the ADCC pathway, such as FcRgamma, CD3zeta, SYK, SHP-1, ZAP70, and the transcription facto …