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674 results

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Page 1
Bacteriophage Isolation and Characterization: Phages of Escherichia coli.
Jofre J, Muniesa M. Jofre J, et al. Methods Mol Biol. 2020;2075:61-79. doi: 10.1007/978-1-4939-9877-7_4. Methods Mol Biol. 2020. PMID: 31584154
Here we introduce methods for the detection, enumeration, and isolation of bacteriophages from Escherichia coli. In bacteria, horizontal gene transfer may be mediated by virulent and temperate phages. ...In temperate phages, the lytic cycle must be activated, …
Here we introduce methods for the detection, enumeration, and isolation of bacteriophages from Escherichia coli. In bacteria, horizon …
Knowing and Naming: Phage Annotation and Nomenclature for Phage Therapy.
Grigson SR, Giles SK, Edwards RA, Papudeshi B. Grigson SR, et al. Clin Infect Dis. 2023 Nov 2;77(Suppl 5):S352-S359. doi: 10.1093/cid/ciad539. Clin Infect Dis. 2023. PMID: 37932119 Free PMC article. Review.
Bacteriophages, or phages, are viruses that infect bacteria shaping microbial communities and ecosystems. ...Accurate phage genome annotation and nomenclature provide insights into phage-host interactions, replication strategies, and evolution, acceler …
Bacteriophages, or phages, are viruses that infect bacteria shaping microbial communities and ecosystems. ...Accurate phage …
A tetravalent TREM2 agonistic antibody reduced amyloid pathology in a mouse model of Alzheimer's disease.
Zhao P, Xu Y, Jiang L, Fan X, Li L, Li X, Arase H, Zhao Y, Cao W, Zheng H, Xu H, Tong Q, Zhang N, An Z. Zhao P, et al. Sci Transl Med. 2022 Sep 7;14(661):eabq0095. doi: 10.1126/scitranslmed.abq0095. Epub 2022 Sep 7. Sci Transl Med. 2022. PMID: 36070367
Triggering receptor expressed on myeloid cells 2 (TREM2) plays crucial roles in Alzheimer's disease (AD) by regulating microglia migration toward, and phagocytosis of oligomeric amyloid-beta (oAbeta) and amyloid plaques. Studies in rodent models of AD have sh …
Triggering receptor expressed on myeloid cells 2 (TREM2) plays crucial roles in Alzheimer's disease (AD) by regulating microglia migration t …
Plaque Size Tool: An automated plaque analysis tool for simplifying and standardising bacteriophage plaque morphology measurements.
Trofimova E, Jaschke PR. Trofimova E, et al. Virology. 2021 Sep;561:1-5. doi: 10.1016/j.virol.2021.05.011. Epub 2021 May 27. Virology. 2021. PMID: 34089996 Free article.
Bacteriophage plaque size measurement is essential for phage characterisation, but manual size estimation requires a considerable amount of time and effort. In order to ease the work of phage researchers, we have developed an automated command-line app …
Bacteriophage plaque size measurement is essential for phage characterisation, but manual size estimation requires a co …
Isolation and molecular characterisation of Achromobacter phage phiAxp-3, an N4-like bacteriophage.
Ma Y, Li E, Qi Z, Li H, Wei X, Lin W, Zhao R, Jiang A, Yang H, Yin Z, Yuan J, Zhao X. Ma Y, et al. Sci Rep. 2016 Apr 20;6:24776. doi: 10.1038/srep24776. Sci Rep. 2016. PMID: 27094846 Free PMC article.
Transmission electron microscopy revealed that, with a 67-nm diameter icosahedral head and a 20-nm non-contractile tail, phiAxp-3 has features characteristic of Podoviridae bacteriophages (order Caudovirales). With a burst size of 9000 plaque-forming units and a lat …
Transmission electron microscopy revealed that, with a 67-nm diameter icosahedral head and a 20-nm non-contractile tail, phiAxp-3 has featur …
The problem of Mycobacterium abscessus complex: multi-drug resistance, bacteriophage susceptibility and potential healthcare transmission.
Dedrick RM, Abad L, Storey N, Kaganovsky AM, Smith BE, Aull HA, Cristinziano M, Morkowska A, Murthy S, Loebinger MR, Hatfull GF, Satta G. Dedrick RM, et al. Clin Microbiol Infect. 2023 Oct;29(10):1335.e9-1335.e16. doi: 10.1016/j.cmi.2023.06.026. Epub 2023 Jun 24. Clin Microbiol Infect. 2023. PMID: 37364635 Free PMC article.
METHODS: Antibiotic susceptibility testing was performed using CLSI breakpoints (n = 95), and plaque assays were used for phage susceptibility testing (subset of n = 88, 35 rough and 53 smooth morphology). ...Rough colony morphotype strains were more phage …
METHODS: Antibiotic susceptibility testing was performed using CLSI breakpoints (n = 95), and plaque assays were used for phage …
A novel phage carrying capsule depolymerase effectively relieves pneumonia caused by multidrug-resistant Klebsiella aerogenes.
Cui X, Du B, Feng J, Feng Y, Fan Z, Chen J, Cui J, Gan L, Fu T, Tian Z, Zhang R, Yan C, Zhao H, Xu W, Xu Z, Yu Z, Ding Z, Li Z, Chen Y, Xue G, Yuan J. Cui X, et al. J Biomed Sci. 2023 Aug 31;30(1):75. doi: 10.1186/s12929-023-00946-y. J Biomed Sci. 2023. PMID: 37653407 Free PMC article.
BACKGROUND: Klebsiella aerogenes can cause ventilator-associated pneumonia by forming biofilms, and it is frequently associated with multidrug resistance. Phages are good antibiotic alternatives with unique advantages. There has been a lack of phage therapeutic expl …
BACKGROUND: Klebsiella aerogenes can cause ventilator-associated pneumonia by forming biofilms, and it is frequently associated with multidr …
In-Gel Isolation and Characterization of Large (and Other) Phages.
Serwer P, Wright ET. Serwer P, et al. Viruses. 2020 Apr 7;12(4):410. doi: 10.3390/v12040410. Viruses. 2020. PMID: 32272774 Free PMC article. Review.
We review some aspects of the rapid isolation of, screening for and characterization of jumbo phages, i.e., phages that have dsDNA genomes longer than 200 Kb. The first aspect is that, as plaque-supporting gels become more concentrated, jumbo phage …
We review some aspects of the rapid isolation of, screening for and characterization of jumbo phages, i.e., phages that have d …
The comparison of lytic activity of isolated phage and commercial Intesti bacteriophage on ESBL producer E. coli and determination of Ec_P6 phage efficacy with in vivo Galleria mellonella larvae model.
Erol HB, Kaskatepe B, Ozturk S, Safi Oz Z. Erol HB, et al. Microb Pathog. 2022 Jun;167:105563. doi: 10.1016/j.micpath.2022.105563. Epub 2022 May 2. Microb Pathog. 2022. PMID: 35513294
The phages were first screened based on plaque morphology and host ranges determined on clinical strains. ...Overall strains were susceptible to one or more phages from the panel. It was proved that Intesti bacteriophage is very effective in a w …
The phages were first screened based on plaque morphology and host ranges determined on clinical strains. ...Overall st …
Phage S144, A New Polyvalent Phage Infecting Salmonella spp. and Cronobacter sakazakii.
Gambino M, Nørgaard Sørensen A, Ahern S, Smyrlis G, Gencay YE, Hendrix H, Neve H, Noben JP, Lavigne R, Brøndsted L. Gambino M, et al. Int J Mol Sci. 2020 Jul 22;21(15):5196. doi: 10.3390/ijms21155196. Int J Mol Sci. 2020. PMID: 32707941 Free PMC article.
Here, we characterize the novel polyvalent phage S144, a member of the Loughboroughvirus genus. By screening 211 Enterobacteriaceae strains, we found that phage S144 forms plaques on specific serovars of Salmonella enterica subsp. enterica and on Cronobacter …
Here, we characterize the novel polyvalent phage S144, a member of the Loughboroughvirus genus. By screening 211 Enterobacteriaceae s …
674 results