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A rapid and efficient DNA extraction protocol from fresh and frozen human blood samples.
Guha P, Das A, Dutta S, Chaudhuri TK. Guha P, et al. J Clin Lab Anal. 2018 Jan;32(1):e22181. doi: 10.1002/jcla.22181. Epub 2017 Feb 23. J Clin Lab Anal. 2018. PMID: 28233351 Free PMC article.
Herein, we aimed to develop a method to extract DNA from 500 muL of fresh or frozen human blood. METHODS: Five hundred microliters of fresh and frozen human blood samples were used for standardization of the extraction procedure. Absorbance at 260 and 280 nm, …
Herein, we aimed to develop a method to extract DNA from 500 muL of fresh or frozen human blood. METHODS: Five hundred microli …
Towards standards for human fecal sample processing in metagenomic studies.
Costea PI, Zeller G, Sunagawa S, Pelletier E, Alberti A, Levenez F, Tramontano M, Driessen M, Hercog R, Jung FE, Kultima JR, Hayward MR, Coelho LP, Allen-Vercoe E, Bertrand L, Blaut M, Brown JRM, Carton T, Cools-Portier S, Daigneault M, Derrien M, Druesne A, de Vos WM, Finlay BB, Flint HJ, Guarner F, Hattori M, Heilig H, Luna RA, van Hylckama Vlieg J, Junick J, Klymiuk I, Langella P, Le Chatelier E, Mai V, Manichanh C, Martin JC, Mery C, Morita H, O'Toole PW, Orvain C, Patil KR, Penders J, Persson S, Pons N, Popova M, Salonen A, Saulnier D, Scott KP, Singh B, Slezak K, Veiga P, Versalovic J, Zhao L, Zoetendal EG, Ehrlich SD, Dore J, Bork P. Costea PI, et al. Nat Biotechnol. 2017 Nov;35(11):1069-1076. doi: 10.1038/nbt.3960. Epub 2017 Oct 2. Nat Biotechnol. 2017. PMID: 28967887
To rank DNA extraction protocols, we considered resulting DNA quantity and quality, and we ascertained biases in estimates of community diversity and the ratio between Gram-positive and Gram-negative bacteria. We recommend a standardized DNA extract
To rank DNA extraction protocols, we considered resulting DNA quantity and quality, and we ascertained biases in estima …
Integrated DNA and RNA extraction using magnetic beads from viral pathogens causing acute respiratory infections.
He H, Li R, Chen Y, Pan P, Tong W, Dong X, Chen Y, Yu D. He H, et al. Sci Rep. 2017 Mar 23;7:45199. doi: 10.1038/srep45199. Sci Rep. 2017. PMID: 28332631 Free PMC article.
Current extraction methods often extract DNA and RNA separately, and few methods are capable of co-extracting DNA and RNA from sputum. ...The feasibility of the simultaneous nucleic acid co-extraction method was evaluated by amplifying …
Current extraction methods often extract DNA and RNA separately, and few methods are capable of co-extracting
Comparing Viral Metagenomic Extraction Methods.
Klenner J, Kohl C, Dabrowski PW, Nitsche A. Klenner J, et al. Curr Issues Mol Biol. 2017;24:59-70. doi: 10.21775/cimb.024.059. Epub 2017 Jul 6. Curr Issues Mol Biol. 2017. PMID: 28686568
A crucial step in the molecular detection of viruses in clinical specimens is the efficient extraction of viral nucleic acids. The total yield of viral nucleic acid from a clinical specimen is dependent on the specimen's volume, the initial virus concentration and t …
A crucial step in the molecular detection of viruses in clinical specimens is the efficient extraction of viral nucleic acids. The to …
Reliability and performance of commercial RNA and DNA extraction kits for FFPE tissue cores.
Patel PG, Selvarajah S, Guérard KP, Bartlett JMS, Lapointe J, Berman DM, Okello JBA, Park PC. Patel PG, et al. PLoS One. 2017 Jun 22;12(6):e0179732. doi: 10.1371/journal.pone.0179732. eCollection 2017. PLoS One. 2017. PMID: 28640876 Free PMC article.
We compared the yield and quality of nucleic acids from 0.6-mm FFPE prostate tissue cores across 16 DNA and RNA extraction protocols, using 14 commercially available kits. ...However, the protocols varied widely in the quality of the extracted RNA and DNA
We compared the yield and quality of nucleic acids from 0.6-mm FFPE prostate tissue cores across 16 DNA and RNA extraction pro …
Direct PCR amplification of forensic touch and other challenging DNA samples: A review.
Cavanaugh SE, Bathrick AS. Cavanaugh SE, et al. Forensic Sci Int Genet. 2018 Jan;32:40-49. doi: 10.1016/j.fsigen.2017.10.005. Epub 2017 Oct 18. Forensic Sci Int Genet. 2018. PMID: 29059581 Review.
DNA evidence sample processing typically involves DNA extraction, quantification, and STR amplification; however, DNA loss can occur at both the DNA extraction and quantification steps, which is not ideal for forensic evidence containing
DNA evidence sample processing typically involves DNA extraction, quantification, and STR amplification; however, DN
New CRISPR-Cas systems from uncultivated microbes.
Burstein D, Harrington LB, Strutt SC, Probst AJ, Anantharaman K, Thomas BC, Doudna JA, Banfield JF. Burstein D, et al. Nature. 2017 Feb 9;542(7640):237-241. doi: 10.1038/nature21059. Epub 2016 Dec 22. Nature. 2017. PMID: 28005056 Free PMC article.
CRISPR-Cas systems provide microbes with adaptive immunity by employing short DNA sequences, termed spacers, that guide Cas proteins to cleave foreign DNA. ...However, current CRISPR-Cas technologies are based solely on systems from isolated bacteria, leaving the va …
CRISPR-Cas systems provide microbes with adaptive immunity by employing short DNA sequences, termed spacers, that guide Cas proteins …
Evaluating genomic DNA extraction methods from human whole blood using endpoint and real-time PCR assays.
Koshy L, Anju AL, Harikrishnan S, Kutty VR, Jissa VT, Kurikesu I, Jayachandran P, Jayakumaran Nair A, Gangaprasad A, Nair GM, Sudhakaran PR. Koshy L, et al. Mol Biol Rep. 2017 Feb;44(1):97-108. doi: 10.1007/s11033-016-4085-9. Epub 2016 Sep 29. Mol Biol Rep. 2017. PMID: 27686559
The extraction of genomic DNA is the crucial first step in large-scale epidemiological studies. ...The appropriate method for efficiently extracting relatively pure DNA was assessed based on the total DNA yield, concentration, purity ratios (A(2 …
The extraction of genomic DNA is the crucial first step in large-scale epidemiological studies. ...The appropriate method for …
Comparison of DNA extraction methods for human gut microbial community profiling.
Lim MY, Song EJ, Kim SH, Lee J, Nam YD. Lim MY, et al. Syst Appl Microbiol. 2018 Mar;41(2):151-157. doi: 10.1016/j.syapm.2017.11.008. Epub 2017 Dec 21. Syst Appl Microbiol. 2018. PMID: 29305057 Free article.
We tested nine different DNA extraction methods using three commercial kits (TianLong Stool DNA/RNA Extraction Kit (TS), QIAamp DNA Stool Mini Kit (QS), and QIAamp PowerFecal DNA Kit (QP)) with or without additional bead-beating step usin …
We tested nine different DNA extraction methods using three commercial kits (TianLong Stool DNA/RNA Extraction K …
Human DNA extraction from whole saliva that was fresh or stored for 3, 6 or 12 months using five different protocols.
Garbieri TF, Brozoski DT, Dionísio TJ, Santos CF, Neves LT. Garbieri TF, et al. J Appl Oral Sci. 2017 Mar-Apr;25(2):147-158. doi: 10.1590/1678-77572016-0046. J Appl Oral Sci. 2017. PMID: 28403355 Free PMC article.
OBJECTIVE AND MATERIAL AND METHODS: This study aimed to compare the quantity and quality of human DNA extracted from saliva that was fresh or frozen for three, six and twelve months using five different DNA extraction protocols: protocol 1 - Oragene co …
OBJECTIVE AND MATERIAL AND METHODS: This study aimed to compare the quantity and quality of human DNA extracted from saliva th …
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