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Improving the generation of genomic-type transgenic mice by ICSI.
Moreira PN, Pozueta J, Pérez-Crespo M, Valdivieso F, Gutiérrez-Adán A, Montoliu L. Moreira PN, et al. Among authors: montoliu l. Transgenic Res. 2007 Apr;16(2):163-8. doi: 10.1007/s11248-007-9075-1. Epub 2007 Feb 16. Transgenic Res. 2007. PMID: 17372844 Review.
Transgenes included in genomic-type constructs, such as yeast artificial chromosomes (YAC), P1-derived artificial chromosomes, or bacterial artificial chromosomes (BAC), are normally correctly expressed, according to the endogenous expression pattern of the homologous locu …
Transgenes included in genomic-type constructs, such as yeast artificial chromosomes (YAC), P1-derived artificial chromosomes, or bac …
Germ line transmission of yeast artificial chromosomes in transgenic mice.
Montoliu L, Schedl A, Kelsey G, Zentgraf H, Lichter P, Schütz G. Montoliu L, et al. Reprod Fertil Dev. 1994;6(5):577-84. doi: 10.1071/rd9940577. Reprod Fertil Dev. 1994. PMID: 7569036 Review.
Different methodological approaches have been shown to produce similar results, namely, the faithful expression of the transgenes carried on YAC DNA. In this paper, we compare the reported techniques for obtaining transgenic mice carrying YACs using a 250-kb YAC bea …
Different methodological approaches have been shown to produce similar results, namely, the faithful expression of the transgenes carried on …
Size matters: use of YACs, BACs and PACs in transgenic animals.
Giraldo P, Montoliu L. Giraldo P, et al. Among authors: montoliu l. Transgenic Res. 2001 Apr;10(2):83-103. doi: 10.1023/a:1008918913249. Transgenic Res. 2001. PMID: 11305364 Review.
The transfer of these large fragments of cloned genomic DNA correlated with optimal expression levels of the transgenes, irrespective of their location in the host genome. Thereafter, other groups confirmed the advantages of YAC transgenesis and position-independent and co …
The transfer of these large fragments of cloned genomic DNA correlated with optimal expression levels of the transgenes, irrespective of the …
Purification of Yeast Artificial Chromosome DNA for Microinjection Using a Two-Gel Electrophoresis Procedure.
Montoliu L. Montoliu L. Cold Spring Harb Protoc. 2018 Aug 1;2018(8). doi: 10.1101/pdb.prot093930. Cold Spring Harb Protoc. 2018. PMID: 30068584
Therefore YAC DNA must always be kept in a solution that maintains the integrity of the YAC DNA molecules. ...The YAC DNA migrates out of the agarose slice and enters into a thicker low-melting agarose gel, thereby promoting compaction of YAC DNA molec …
Therefore YAC DNA must always be kept in a solution that maintains the integrity of the YAC DNA molecules. ...The YAC D …
New animal models to study the role of tyrosinase in normal retinal development.
Lavado A, Montoliu L. Lavado A, et al. Among authors: montoliu l. Front Biosci. 2006 Jan 1;11:743-52. doi: 10.2741/1832. Front Biosci. 2006. PMID: 16146766 Review.
Second, we will comment on an interesting yeast artificial chromosome (YAC)-tyrosinase transgene, lacking important regulatory elements, that has highlighted the significance of local interactions between the retinal pigment epithelium (RPE) and developing neural retina... …
Second, we will comment on an interesting yeast artificial chromosome (YAC)-tyrosinase transgene, lacking important regulatory elemen …
Purification of Yeast Artificial Chromosome DNA for Microinjection Using Pulsed-Field Gel Electrophoresis and Ultrafiltration.
Montoliu L. Montoliu L. Cold Spring Harb Protoc. 2018 Aug 1;2018(8). doi: 10.1101/pdb.prot093948. Cold Spring Harb Protoc. 2018. PMID: 30068585
The agarose slice containing the YAC DNA of interest is melted and digested. The resulting YAC DNA-containing solution is concentrated using ultrafiltration units with a 30,000-mwt cutoff. The DNA is retained in the upper container, where it concentrates sixfold to …
The agarose slice containing the YAC DNA of interest is melted and digested. The resulting YAC DNA-containing solution is conc …
YAC transgenesis in farm animals: rescue of albinism in rabbits.
Brem G, Besenfelder U, Aigner B, Müller M, Liebl I, Schütz G, Montoliu L. Brem G, et al. Among authors: montoliu l. Mol Reprod Dev. 1996 May;44(1):56-62. doi: 10.1002/(SICI)1098-2795(199605)44:1<56::AID-MRD6>3.0.CO;2-S. Mol Reprod Dev. 1996. PMID: 8722692
With the experiments discussed in this report, we have addressed the possibility of applying the obvious advantages of YAC transgenesis to farm animals. We have generated transgenic rabbits carrying a 250 kb YAC covering the mouse tyrosinase gene by pronuclear micro …
With the experiments discussed in this report, we have addressed the possibility of applying the obvious advantages of YAC transgenes …
A method for the generation of YAC transgenic mice by pronuclear microinjection.
Schedl A, Larin Z, Montoliu L, Thies E, Kelsey G, Lehrach H, Schütz G. Schedl A, et al. Among authors: montoliu l. Nucleic Acids Res. 1993 Oct 11;21(20):4783-7. doi: 10.1093/nar/21.20.4783. Nucleic Acids Res. 1993. PMID: 8233827 Free PMC article.
Microinjection into the nucleus is the most direct and efficient way of delivering YAC DNA into cells, but requires the purification of the YAC from the remaining yeast chromosomes. ...Introduction by homologous recombination in yeast of sequences permitting YAC
Microinjection into the nucleus is the most direct and efficient way of delivering YAC DNA into cells, but requires the purification …
Large-Scale Preparation of Yeast Agarose Plugs to Isolate Yeast Artificial Chromosome DNA.
Montoliu L. Montoliu L. Cold Spring Harb Protoc. 2018 Aug 1;2018(8). doi: 10.1101/pdb.prot093955. Cold Spring Harb Protoc. 2018. PMID: 30068586
This protocol describes how to prepare large-scale yeast agarose plugs to isolate YAC DNA. The quality of the YAC DNA preparation is the most important consideration in optimizing transgenic mouse production....
This protocol describes how to prepare large-scale yeast agarose plugs to isolate YAC DNA. The quality of the YAC DNA preparat …
A yeast artificial chromosome covering the tyrosinase gene confers copy number-dependent expression in transgenic mice.
Schedl A, Montoliu L, Kelsey G, Schütz G. Schedl A, et al. Among authors: montoliu l. Nature. 1993 Mar 18;362(6417):258-61. doi: 10.1038/362258a0. Nature. 1993. PMID: 8459851
Here we report the transfer of a 250 kilobase YAC covering the mouse tyrosinase gene into mice by pronuclear injection of gel-purified YAC DNA. The YAC was inserted into the mouse genome without major rearrangements and expression of the YAC-borne tyro …
Here we report the transfer of a 250 kilobase YAC covering the mouse tyrosinase gene into mice by pronuclear injection of gel-purifie …
17 results