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Purification of Yeast Artificial Chromosome DNA for Microinjection Using a Two-Gel Electrophoresis Procedure.
Montoliu L. Montoliu L. Cold Spring Harb Protoc. 2018 Aug 1;2018(8). doi: 10.1101/pdb.prot093930. Cold Spring Harb Protoc. 2018. PMID: 30068584
Therefore YAC DNA must always be kept in a solution that maintains the integrity of the YAC DNA molecules. ...The YAC DNA migrates out of the agarose slice and enters into a thicker low-melting agarose gel, thereby promoting compaction of YAC DNA molec …
Therefore YAC DNA must always be kept in a solution that maintains the integrity of the YAC DNA molecules. ...The YAC D …
Purification of Yeast Artificial Chromosome DNA for Microinjection Using Pulsed-Field Gel Electrophoresis and Ultrafiltration.
Montoliu L. Montoliu L. Cold Spring Harb Protoc. 2018 Aug 1;2018(8). doi: 10.1101/pdb.prot093948. Cold Spring Harb Protoc. 2018. PMID: 30068585
The agarose slice containing the YAC DNA of interest is melted and digested. The resulting YAC DNA-containing solution is concentrated using ultrafiltration units with a 30,000-mwt cutoff. The DNA is retained in the upper container, where it concentrates sixfold to …
The agarose slice containing the YAC DNA of interest is melted and digested. The resulting YAC DNA-containing solution is conc …
Improving the generation of genomic-type transgenic mice by ICSI.
Moreira PN, Pozueta J, Pérez-Crespo M, Valdivieso F, Gutiérrez-Adán A, Montoliu L. Moreira PN, et al. Among authors: montoliu l. Transgenic Res. 2007 Apr;16(2):163-8. doi: 10.1007/s11248-007-9075-1. Epub 2007 Feb 16. Transgenic Res. 2007. PMID: 17372844 Review.
Transgenes included in genomic-type constructs, such as yeast artificial chromosomes (YAC), P1-derived artificial chromosomes, or bacterial artificial chromosomes (BAC), are normally correctly expressed, according to the endogenous expression pattern of the homologous locu …
Transgenes included in genomic-type constructs, such as yeast artificial chromosomes (YAC), P1-derived artificial chromosomes, or bac …
Size matters: use of YACs, BACs and PACs in transgenic animals.
Giraldo P, Montoliu L. Giraldo P, et al. Among authors: montoliu l. Transgenic Res. 2001 Apr;10(2):83-103. doi: 10.1023/a:1008918913249. Transgenic Res. 2001. PMID: 11305364 Review.
The transfer of these large fragments of cloned genomic DNA correlated with optimal expression levels of the transgenes, irrespective of their location in the host genome. Thereafter, other groups confirmed the advantages of YAC transgenesis and position-independent and co …
The transfer of these large fragments of cloned genomic DNA correlated with optimal expression levels of the transgenes, irrespective of the …
Large-Scale Preparation of Yeast Agarose Plugs to Isolate Yeast Artificial Chromosome DNA.
Montoliu L. Montoliu L. Cold Spring Harb Protoc. 2018 Aug 1;2018(8). doi: 10.1101/pdb.prot093955. Cold Spring Harb Protoc. 2018. PMID: 30068586
This protocol describes how to prepare large-scale yeast agarose plugs to isolate YAC DNA. The quality of the YAC DNA preparation is the most important consideration in optimizing transgenic mouse production....
This protocol describes how to prepare large-scale yeast agarose plugs to isolate YAC DNA. The quality of the YAC DNA preparat …
A method for the generation of YAC transgenic mice by pronuclear microinjection.
Schedl A, Larin Z, Montoliu L, Thies E, Kelsey G, Lehrach H, Schütz G. Schedl A, et al. Among authors: montoliu l. Nucleic Acids Res. 1993 Oct 11;21(20):4783-7. doi: 10.1093/nar/21.20.4783. Nucleic Acids Res. 1993. PMID: 8233827 Free PMC article.
Microinjection into the nucleus is the most direct and efficient way of delivering YAC DNA into cells, but requires the purification of the YAC from the remaining yeast chromosomes. ...Introduction by homologous recombination in yeast of sequences permitting YAC
Microinjection into the nucleus is the most direct and efficient way of delivering YAC DNA into cells, but requires the purification …
New animal models to study the role of tyrosinase in normal retinal development.
Lavado A, Montoliu L. Lavado A, et al. Among authors: montoliu l. Front Biosci. 2006 Jan 1;11:743-52. doi: 10.2741/1832. Front Biosci. 2006. PMID: 16146766 Review.
Second, we will comment on an interesting yeast artificial chromosome (YAC)-tyrosinase transgene, lacking important regulatory elements, that has highlighted the significance of local interactions between the retinal pigment epithelium (RPE) and developing neural retina... …
Second, we will comment on an interesting yeast artificial chromosome (YAC)-tyrosinase transgene, lacking important regulatory elemen …
Efficient generation of transgenic mice with intact yeast artificial chromosomes by intracytoplasmic sperm injection.
Moreira PN, Giraldo P, Cozar P, Pozueta J, Jiménez A, Montoliu L, Gutiérrez-Adán A. Moreira PN, et al. Among authors: montoliu l. Biol Reprod. 2004 Dec;71(6):1943-7. doi: 10.1095/biolreprod.104.032904. Epub 2004 Jul 30. Biol Reprod. 2004. PMID: 15286029 Free article.
In the present study, we describe an intracytoplasmic sperm injection (ICSI) method for the stable incorporation and phenotypic expression of large yeast artificial chromosomes (YAC) constructs of submegabase and megabase magnitude. By coinjecting spermatozoa and YACs into …
In the present study, we describe an intracytoplasmic sperm injection (ICSI) method for the stable incorporation and phenotypic expression o …
A yeast artificial chromosome covering the tyrosinase gene confers copy number-dependent expression in transgenic mice.
Schedl A, Montoliu L, Kelsey G, Schütz G. Schedl A, et al. Among authors: montoliu l. Nature. 1993 Mar 18;362(6417):258-61. doi: 10.1038/362258a0. Nature. 1993. PMID: 8459851
Here we report the transfer of a 250 kilobase YAC covering the mouse tyrosinase gene into mice by pronuclear injection of gel-purified YAC DNA. The YAC was inserted into the mouse genome without major rearrangements and expression of the YAC-borne tyro …
Here we report the transfer of a 250 kilobase YAC covering the mouse tyrosinase gene into mice by pronuclear injection of gel-purifie …
Generation of yeast artificial chromosome transgenic mice by intracytoplasmic sperm injection.
Moreira PN, Pozueta J, Giraldo P, Gutiérrez-Adán A, Montoliu L. Moreira PN, et al. Among authors: montoliu l. Methods Mol Biol. 2006;349:151-61. doi: 10.1385/1-59745-158-4:151. Methods Mol Biol. 2006. PMID: 17071981
The largest genomic transgenes can be prepared using yeast artificial chromosomes (YACs). Normally, YAC transgenic mice are produced by standard pro-nuclear microinjection, although other methods, involving the use of embryonic stem (ES) cells, have been also devised. To o …
The largest genomic transgenes can be prepared using yeast artificial chromosomes (YACs). Normally, YAC transgenic mice are produced …
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