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2019 12
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2,427 results
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Comparison of isolation methods of exosomes and exosomal RNA from cell culture medium and serum.
Tang YT, Huang YY, Zheng L, Qin SH, Xu XP, An TX, Xu Y, Wu YS, Hu XM, Ping BH, Wang Q. Tang YT, et al. Int J Mol Med. 2017 Sep;40(3):834-844. doi: 10.3892/ijmm.2017.3080. Epub 2017 Jul 24. Int J Mol Med. 2017. PMID: 28737826 Free PMC article.
Exosomes in cell culture medium (CCM) and serum may be isolated using ultracentrifugation (UC), ExoQuick or Total Exosome Isolation Reagent (TEI), and exoRNA may be extracted using TRIzol-LS, SeraMir, Total Exosome RNA Isolation (TER), HiPure Liquid RNA/miRNA …
Exosomes in cell culture medium (CCM) and serum may be isolated using ultracentrifugation (UC), ExoQuick or Total Exosome Isolation Reagent …
Translation of CircRNAs.
Pamudurti NR, Bartok O, Jens M, Ashwal-Fluss R, Stottmeister C, Ruhe L, Hanan M, Wyler E, Perez-Hernandez D, Ramberger E, Shenzis S, Samson M, Dittmar G, Landthaler M, Chekulaeva M, Rajewsky N, Kadener S. Pamudurti NR, et al. Mol Cell. 2017 Apr 6;66(1):9-21.e7. doi: 10.1016/j.molcel.2017.02.021. Epub 2017 Mar 23. Mol Cell. 2017. PMID: 28344080 Free PMC article.
In addition, we found that a circRNA generated from the muscleblind locus encodes a protein, which we detected in fly head extracts by mass spectrometry. Next, by performing in vivo and in vitro translation assays, we show that UTRs of ribo-circRNAs (cUTRs) allow cap-indep …
In addition, we found that a circRNA generated from the muscleblind locus encodes a protein, which we detected in fly head extracts b …
Integrated DNA and RNA extraction using magnetic beads from viral pathogens causing acute respiratory infections.
He H, Li R, Chen Y, Pan P, Tong W, Dong X, Chen Y, Yu D. He H, et al. Sci Rep. 2017 Mar 23;7:45199. doi: 10.1038/srep45199. Sci Rep. 2017. PMID: 28332631 Free PMC article.
Current extraction methods often extract DNA and RNA separately, and few methods are capable of co-extracting DNA and RNA from sputum. ...Both DNA and RNA were most efficiently extracted when the GTC and DTT concentrations were 2.0 …
Current extraction methods often extract DNA and RNA separately, and few methods are capable of co-extracting DN …
Reliability and performance of commercial RNA and DNA extraction kits for FFPE tissue cores.
Patel PG, Selvarajah S, Guérard KP, Bartlett JMS, Lapointe J, Berman DM, Okello JBA, Park PC. Patel PG, et al. PLoS One. 2017 Jun 22;12(6):e0179732. doi: 10.1371/journal.pone.0179732. eCollection 2017. PLoS One. 2017. PMID: 28640876 Free PMC article.
We compared the yield and quality of nucleic acids from 0.6-mm FFPE prostate tissue cores across 16 DNA and RNA extraction protocols, using 14 commercially available kits. ...However, the protocols varied widely in the quality of the extracted RNA and …
We compared the yield and quality of nucleic acids from 0.6-mm FFPE prostate tissue cores across 16 DNA and RNA extraction pro …
Comparing Viral Metagenomic Extraction Methods.
Klenner J, Kohl C, Dabrowski PW, Nitsche A. Klenner J, et al. Curr Issues Mol Biol. 2017;24:59-70. doi: 10.21775/cimb.024.059. Epub 2017 Jul 6. Curr Issues Mol Biol. 2017. PMID: 28686568
A crucial step in the molecular detection of viruses in clinical specimens is the efficient extraction of viral nucleic acids. The total yield of viral nucleic acid from a clinical specimen is dependent on the specimen's volume, the initial virus concentration and t …
A crucial step in the molecular detection of viruses in clinical specimens is the efficient extraction of viral nucleic acids. The to …
[Application of RT-qPCR in the Study of Forensic Pathology].
Du SH, Li DR, Wang HJ, Wang Q. Du SH, et al. Fa Yi Xue Za Zhi. 2017 Oct;33(5):526-531. doi: 10.3969/j.issn.1004-5619.2017.05.017. Epub 2017 Oct 25. Fa Yi Xue Za Zhi. 2017. PMID: 29275561 Review. Chinese.
This paper reviews the application value of RT-qPCR in the study of forensic pathology and current situation, as well as the research progress at home and abroad reviews. It also summarizes the notes of samples extraction, RT-qPCR experiments and data processing, which aim …
This paper reviews the application value of RT-qPCR in the study of forensic pathology and current situation, as well as the research progre …
A Compendium of RNA-Binding Proteins that Regulate MicroRNA Biogenesis.
Treiber T, Treiber N, Plessmann U, Harlander S, Daiß JL, Eichner N, Lehmann G, Schall K, Urlaub H, Meister G. Treiber T, et al. Mol Cell. 2017 Apr 20;66(2):270-284.e13. doi: 10.1016/j.molcel.2017.03.014. Mol Cell. 2017. PMID: 28431233 Free article.
These processing steps can be posttranscriptionally regulated by RNA-binding proteins (RBPs). Here, we have used a proteomics-based pull-down approach to map and characterize the interactome of a multitude of pre-miRNAs. ...We provide an extended database of RBPs that inte …
These processing steps can be posttranscriptionally regulated by RNA-binding proteins (RBPs). Here, we have used a proteomics-based p …
A Comparative Study of Serum Exosome Isolation Using Differential Ultracentrifugation and Three Commercial Reagents.
Helwa I, Cai J, Drewry MD, Zimmerman A, Dinkins MB, Khaled ML, Seremwe M, Dismuke WM, Bieberich E, Stamer WD, Hamrick MW, Liu Y. Helwa I, et al. PLoS One. 2017 Jan 23;12(1):e0170628. doi: 10.1371/journal.pone.0170628. eCollection 2017. PLoS One. 2017. PMID: 28114422 Free PMC article.
The isolated exosomes were characterized based upon size, quantity, zeta potential, CD63 and CD9 protein expression, and exosomal RNA (exRNA) quality and quantity using several complementary methods: nanoparticle tracking analysis (NTA) with ZetaView, western blot, transmi …
The isolated exosomes were characterized based upon size, quantity, zeta potential, CD63 and CD9 protein expression, and exosomal RNA
Nucleic acid purification from plants, animals and microbes in under 30 seconds.
Zou Y, Mason MG, Wang Y, Wee E, Turni C, Blackall PJ, Trau M, Botella JR. Zou Y, et al. PLoS Biol. 2017 Nov 21;15(11):e2003916. doi: 10.1371/journal.pbio.2003916. eCollection 2017 Nov. PLoS Biol. 2017. PMID: 29161268 Free PMC article.
Building on this knowledge, we have successfully created an equipment-free nucleic acid extraction dipstick methodology that can obtain amplification-ready DNA and RNA from plants, animals, and microbes from difficult biological samples such as blood and leaves from …
Building on this knowledge, we have successfully created an equipment-free nucleic acid extraction dipstick methodology that can obta …
Evaluation of Methods for the Concentration and Extraction of Viruses from Sewage in the Context of Metagenomic Sequencing.
Hjelmsø MH, Hellmér M, Fernandez-Cassi X, Timoneda N, Lukjancenko O, Seidel M, Elsässer D, Aarestrup FM, Löfström C, Bofill-Mas S, Abril JF, Girones R, Schultz AC. Hjelmsø MH, et al. PLoS One. 2017 Jan 18;12(1):e0170199. doi: 10.1371/journal.pone.0170199. eCollection 2017. PLoS One. 2017. PMID: 28099518 Free PMC article.
We found a significant influence of concentration and extraction protocols on the detected viriome. The viral richness was largest in samples extracted with QIAamp Viral RNA Mini Kit or PowerViral Environmental RNA/DNA Isolation Kit. Highest viral spec …
We found a significant influence of concentration and extraction protocols on the detected viriome. The viral richness was largest in …
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