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2014 1
2016 5
2017 308
2018 2570
2019 292
2020 8
2021 2
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2,570 results
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High Throughput Single Cell RNA Sequencing, Bioinformatics Analysis and Applications.
Huang X, Liu S, Wu L, Jiang M, Hou Y. Huang X, et al. Adv Exp Med Biol. 2018;1068:33-43. doi: 10.1007/978-981-13-0502-3_4. Adv Exp Med Biol. 2018. PMID: 29943294 Review.
Next generation sequencing (NGS) technology is the driving force for single cell sequencing. scRNA-seq requires a lengthy pipeline comprising of single cell sorting, RNA extraction, reverse transcription, amplification, library construction, sequencing and subsequen …
Next generation sequencing (NGS) technology is the driving force for single cell sequencing. scRNA-seq requires a lengthy pipeline comprisin …
Evaluation of commercial DNA and RNA extraction methods for high-throughput sequencing of FFPE samples.
Kresse SH, Namløs HM, Lorenz S, Berner JM, Myklebost O, Bjerkehagen B, Meza-Zepeda LA. Kresse SH, et al. PLoS One. 2018 May 17;13(5):e0197456. doi: 10.1371/journal.pone.0197456. eCollection 2018. PLoS One. 2018. PMID: 29771965 Free PMC article.
To identify optimal nucleic acid extraction kits, DNA and RNA quantity, quality and performance in HTS applications were evaluated. ...For RNA extraction, all protocols gave comparable yields and amplifiable RNA. ...
To identify optimal nucleic acid extraction kits, DNA and RNA quantity, quality and performance in HTS applications were evalu …
Mouse Adipose Tissue Collection and Processing for RNA Analysis.
Tan P, Pepin É, Lavoie JL. Tan P, et al. J Vis Exp. 2018 Jan 31;(131):57026. doi: 10.3791/57026. J Vis Exp. 2018. PMID: 29443083 Free PMC article.
RNA isolation from adipose tissue samples is also challenging as extra steps are required to avoid these lipids. ...It has also been optimized to ensure adequate quantity and quality of the RNA extracted. This protocol can be widely applied to any mouse model
RNA isolation from adipose tissue samples is also challenging as extra steps are required to avoid these lipids. ...It has also been
An overview of technical considerations when using quantitative real-time PCR analysis of gene expression in human exercise research.
Kuang J, Yan X, Genders AJ, Granata C, Bishop DJ. Kuang J, et al. PLoS One. 2018 May 10;13(5):e0196438. doi: 10.1371/journal.pone.0196438. eCollection 2018. PLoS One. 2018. PMID: 29746477 Free PMC article. Review.
Interestingly, we found that mishandling of muscle for a short time span (10 mins) before RNA extraction did not affect RNA quality, and isolated total RNA was preserved for up to one week at room temperature. ...Alternatively, cDNA content can be used …
Interestingly, we found that mishandling of muscle for a short time span (10 mins) before RNA extraction did not affect RNA
Extraction and Analysis of RNA Isolated from Pure Bacteria-Derived Outer Membrane Vesicles.
Habier J, May P, Heintz-Buschart A, Ghosal A, Wienecke-Baldacchino AK, Nolte-'t Hoen ENM, Wilmes P, Fritz JV. Habier J, et al. Methods Mol Biol. 2018;1737:213-230. doi: 10.1007/978-1-4939-7634-8_13. Methods Mol Biol. 2018. PMID: 29484596
These vesicles contain numerous bacterial components, such as proteins, peptidoglycans, lipopolysaccharides, DNA, and RNA. To examine if OMV-associated RNA molecules are bacterial degradation products and/or are functionally active, it is necessary to extract
These vesicles contain numerous bacterial components, such as proteins, peptidoglycans, lipopolysaccharides, DNA, and RNA. To examine …
MicroRNA Analysis Using Next-Generation Sequencing.
Kolanowska M, Kubiak A, Jażdżewski K, Wójcicka A. Kolanowska M, et al. Methods Mol Biol. 2018;1823:87-101. doi: 10.1007/978-1-4939-8624-8_8. Methods Mol Biol. 2018. PMID: 29959676
Next-generation sequencing (NGS) enables the analysis of both microRNA expression and sequence, allowing for elucidation of a comprehensive landscape of miRNAs in a given tissue and sample type. NGS analysis requires high-quality RNA extraction and preparation of mi …
Next-generation sequencing (NGS) enables the analysis of both microRNA expression and sequence, allowing for elucidation of a comprehensive …
Metagenomic analysis of viral nucleic acid extraction methods in respiratory clinical samples.
Zhang D, Lou X, Yan H, Pan J, Mao H, Tang H, Shu Y, Zhao Y, Liu L, Li J, Chen J, Zhang Y, Ma X. Zhang D, et al. BMC Genomics. 2018 Oct 25;19(1):773. doi: 10.1186/s12864-018-5152-5. BMC Genomics. 2018. PMID: 30359242 Free PMC article.
RESULTS: In this study, we performed qRT-PCR and viral metagenomic analysis of the extraction efficiency of four commonly used Qiagen extraction kits: QIAamp Viral RNA Mini Kit (VRMK), QIAamp MinElute Virus Spin Kit (MVSK), RNeasy Mini Kit (RMK), and RNeasy P …
RESULTS: In this study, we performed qRT-PCR and viral metagenomic analysis of the extraction efficiency of four commonly used Qiagen …
DeepM6ASeq: prediction and characterization of m6A-containing sequences using deep learning.
Zhang Y, Hamada M. Zhang Y, et al. BMC Bioinformatics. 2018 Dec 31;19(Suppl 19):524. doi: 10.1186/s12859-018-2516-4. BMC Bioinformatics. 2018. PMID: 30598068 Free PMC article.
BACKGROUND: N6-methyladensine (m6A) is a common and abundant RNA methylation modification found in various species. As a type of post-transcriptional methylation, m6A plays an important role in diverse RNA activities such as alternative splicing, an interplay with m …
BACKGROUND: N6-methyladensine (m6A) is a common and abundant RNA methylation modification found in various species. As a type of post …
Assessment of influenza virus exposure and recovery from contaminated surgical masks and N95 respirators.
Blachere FM, Lindsley WG, McMillen CM, Beezhold DH, Fisher EM, Shaffer RE, Noti JD. Blachere FM, et al. J Virol Methods. 2018 Oct;260:98-106. doi: 10.1016/j.jviromet.2018.05.009. Epub 2018 Jul 17. J Virol Methods. 2018. PMID: 30029810 Free PMC article.
The inclusion of 0.1% SDS resulted in maximal influenza RNA recovery (41.3%) but with a complete loss of infectivity whereas inclusion of 0.1% bovine serum albumin resulted in reduced RNA recovery (6.8%) but maximal retention of virus infectivity (17.9%). Our result …
The inclusion of 0.1% SDS resulted in maximal influenza RNA recovery (41.3%) but with a complete loss of infectivity whereas inclusio …
Immunoprecipitation and SDS-PAGE for Cross-Linking Immunoprecipitation (CLIP).
Darnell JC, Mele A, Hung KYS, Darnell RB. Darnell JC, et al. Cold Spring Harb Protoc. 2018 Dec 3;2018(12). doi: 10.1101/pdb.prot097956. Cold Spring Harb Protoc. 2018. PMID: 30510126
The pilot experiment will identify the optimal RNase concentration for the particular sample and will assess the quality and purity of the RNABP-RNA complexes following labeling of the RNA tags with (32)P. This can be done without ligation of the 3' linker as descri …
The pilot experiment will identify the optimal RNase concentration for the particular sample and will assess the quality and purity of the R …
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