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1993 1
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2019 3291
2020 76
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Analyzing protein glycosylation using UHPLC: a review.
Rowe L, Burkhart G. Rowe L, et al. Bioanalysis. 2018 Oct 1;10(20):1691-1703. doi: 10.4155/bio-2018-0156. Bioanalysis. 2018. PMID: 30412689 Review.
Ultrahigh performance liquid chromatography (UHPLC) uses small stationary-phase particle size (<2 mum) and high pressure in order to achieve rapid and efficient separations. ...
Ultrahigh performance liquid chromatography (UHPLC) uses small stationary-phase particle size (<2 mum) and high pressure in order …
Affinity chromatography for vaccines manufacturing: Finally ready for prime time?
Zhao M, Vandersluis M, Stout J, Haupts U, Sanders M, Jacquemart R. Zhao M, et al. Vaccine. 2019 Aug 23;37(36):5491-5503. doi: 10.1016/j.vaccine.2018.02.090. Epub 2018 Apr 5. Vaccine. 2019. PMID: 29627235 Free article. Review.
Affinity chromatography is among the most powerful separation techniques, achieving the finest separation with high yields even in the most challenging feed streams. ...Despite the success in laboratory-scale proof of concept, implementation of this technique in pilot or c …
Affinity chromatography is among the most powerful separation techniques, achieving the finest separation with high yields even in th …
Untargeted GC-MS Metabolomics.
Papadimitropoulos MP, Vasilopoulou CG, Maga-Nteve C, Klapa MI. Papadimitropoulos MP, et al. Methods Mol Biol. 2018;1738:133-147. doi: 10.1007/978-1-4939-7643-0_9. Methods Mol Biol. 2018. PMID: 29654587
., tissue, biological fluid, cell culture) based on the concentration profile of all measurable free low molecular weight metabolites. Gas chromatography-mass spectrometry (GC-MS), being a highly sensitive and high-throughput analytical platform, has been proven a useful t …
., tissue, biological fluid, cell culture) based on the concentration profile of all measurable free low molecular weight metabolites. Gas …
Advances in native high-performance liquid chromatography and intact mass spectrometry for the characterization of biopharmaceutical products.
Tassi M, De Vos J, Chatterjee S, Sobott F, Bones J, Eeltink S. Tassi M, et al. J Sep Sci. 2018 Jan;41(1):125-144. doi: 10.1002/jssc.201700988. Epub 2017 Nov 2. J Sep Sci. 2018. PMID: 28990739 Free article. Review.
Native liquid-chromatographic modes that are discussed include aqueous size-exclusion chromatography, hydrophobic interaction chromatography, and ion-exchange chromatography. Infusion conditions and the possibilities and limitations to hyphenate native liquid …
Native liquid-chromatographic modes that are discussed include aqueous size-exclusion chromatography, hydrophobic interaction chro …
An overview of experimental designs in HPLC method development and validation.
Sahu PK, Ramisetti NR, Cecchi T, Swain S, Patro CS, Panda J. Sahu PK, et al. J Pharm Biomed Anal. 2018 Jan 5;147:590-611. doi: 10.1016/j.jpba.2017.05.006. Epub 2017 May 7. J Pharm Biomed Anal. 2018. PMID: 28579052 Review.
The present manuscript discusses usefulness of various chemometric approaches in high and ultra performance liquid chromatography for (i) methods development from dissolution studies and sample preparation to detection, considering the progressive substitution of tradition …
The present manuscript discusses usefulness of various chemometric approaches in high and ultra performance liquid chromatography for …
Menstrual cycle rhythmicity: metabolic patterns in healthy women.
Draper CF, Duisters K, Weger B, Chakrabarti A, Harms AC, Brennan L, Hankemeier T, Goulet L, Konz T, Martin FP, Moco S, van der Greef J. Draper CF, et al. Sci Rep. 2018 Oct 1;8(1):14568. doi: 10.1038/s41598-018-32647-0. Sci Rep. 2018. PMID: 30275458 Free PMC article.
A brief practical review of size exclusion chromatography: Rules of thumb, limitations, and troubleshooting.
Burgess RR. Burgess RR. Protein Expr Purif. 2018 Oct;150:81-85. doi: 10.1016/j.pep.2018.05.007. Epub 2018 May 17. Protein Expr Purif. 2018. PMID: 29777748 Review.
I have also found that many researchers do not understand the basics, let alone the pitfalls, of the techniques they use. In the case of size exclusion chromatography (SEC), I summarize below some of the rules of thumb, suggestions, limitations and troubleshooting I have f …
I have also found that many researchers do not understand the basics, let alone the pitfalls, of the techniques they use. In the case of siz …
Analytical methods for cholesterol quantification.
Li LH, Dutkiewicz EP, Huang YC, Zhou HB, Hsu CC. Li LH, et al. J Food Drug Anal. 2019 Apr;27(2):375-386. doi: 10.1016/j.jfda.2018.09.001. Epub 2018 Oct 4. J Food Drug Anal. 2019. PMID: 30987710 Free article. Review.
Multiple analytical methods have been developed for analysis of cholesterol, including classical chemical methods, enzymatic assays, gas chromatography (GC), liquid chromatography (LC), and mass spectrometry (MS). ...
Multiple analytical methods have been developed for analysis of cholesterol, including classical chemical methods, enzymatic assays, gas …
Size-exclusion chromatography using reverse-phase columns for protein separation.
Huang TY, Chi LM, Chien KY. Huang TY, et al. J Chromatogr A. 2018 Oct 12;1571:201-212. doi: 10.1016/j.chroma.2018.08.020. Epub 2018 Aug 10. J Chromatogr A. 2018. PMID: 30146374
Reverse-phase (RP) liquid chromatography (RPLC) and size-exclusion chromatography (SEC) are methods commonly used for protein/peptide separation, and they are based on distinct principles. ...
Reverse-phase (RP) liquid chromatography (RPLC) and size-exclusion chromatography (SEC) are methods commonly used for protein/ …
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