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Road to precision: recombinase-based targeting technologies for genome engineering.
Wirth D, Gama-Norton L, Riemer P, Sandhu U, Schucht R, Hauser H. Wirth D, et al. Among authors: schucht r. Curr Opin Biotechnol. 2007 Oct;18(5):411-9. doi: 10.1016/j.copbio.2007.07.013. Epub 2007 Sep 27. Curr Opin Biotechnol. 2007. PMID: 17904350 Review.
In the past years, recombinase-based approaches for integrating transgenes into defined chromosomal loci of mammalian cells have gained increasing attention. ...This review focuses on the current state of targeting strategies including RMCE employing site-specific recom …
In the past years, recombinase-based approaches for integrating transgenes into defined chromosomal loci of mammalian cells have gain …
Recombinant protein expression by targeting pre-selected chromosomal loci.
Nehlsen K, Schucht R, da Gama-Norton L, Krömer W, Baer A, Cayli A, Hauser H, Wirth D. Nehlsen K, et al. Among authors: schucht r. BMC Biotechnol. 2009 Dec 14;9:100. doi: 10.1186/1472-6750-9-100. BMC Biotechnol. 2009. PMID: 20003421 Free PMC article.
BACKGROUND: Recombinant protein expression in mammalian cells is mostly achieved by stable integration of transgenes into the chromosomal DNA of established cell lines. ...Selected integration sites that had been flanked with heterologous recombinase target sites (F …
BACKGROUND: Recombinant protein expression in mammalian cells is mostly achieved by stable integration of transgenes into the chromos …
Rapid establishment of G-protein-coupled receptor-expressing cell lines by site-specific integration.
Schucht R, Lydford S, Andzinski L, Zauers J, Cooper J, Hauser H, Wirth D, May T. Schucht R, et al. J Biomol Screen. 2011 Mar;16(3):323-31. doi: 10.1177/1087057110396371. Epub 2011 Feb 18. J Biomol Screen. 2011. PMID: 21335600 Free article.
In the second step, this reporter gene was exchanged for that of the GPCR of interest by a DNA recombinase "cut-and-paste" engineering step. It has been demonstrated that the resulting GPCR cell lines inherit the advantages of the master cell line, expressing the GPCR in a …
In the second step, this reporter gene was exchanged for that of the GPCR of interest by a DNA recombinase "cut-and-paste" engineerin …