Abstract
In a proteomics-based screen for proteins interacting with cyclin-dependent protein kinase (CDK), we have identified a novel CDK complex containing the eukaryotic translation initiation factor, eIF4A. Reciprocal immunoprecipitations using antibodies against eIF4A indicate that the interaction is specific. The CDKA-eIF4A complex is abundant in actively proliferating and growing cells but is absent from cells that have ceased dividing. The CDKA-eIF4A complex contains kinase activity that is sensitive to the CDK-specific inhibitor roscovitine. This interaction points to a possible molecular mechanism linking cell proliferation with translational control.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Arabidopsis / cytology
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Arabidopsis / enzymology
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Blotting, Western
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Cell Division / physiology*
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Cyclin-Dependent Kinases / chemistry
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Cyclin-Dependent Kinases / metabolism*
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Enzyme Inhibitors / pharmacology
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Eukaryotic Initiation Factor-4A / chemistry
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Eukaryotic Initiation Factor-4A / metabolism*
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Nicotiana / cytology
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Nicotiana / enzymology
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Precipitin Tests / methods
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Protein Biosynthesis / physiology*
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Protein Kinase Inhibitors
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Protein Kinases / metabolism
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Purines / pharmacology
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Roscovitine
Substances
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Enzyme Inhibitors
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Protein Kinase Inhibitors
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Purines
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Roscovitine
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Protein Kinases
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histone H1 kinase
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Cyclin-Dependent Kinases
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Eukaryotic Initiation Factor-4A