Characterization of Rrh4273I, a restriction-modification system of Rhodococcus rhodochrous ATCC 4273 (Nocardia corallina) which recognizes the same sequence as the Streptomyces albus G SalI restriction-modification system

J Gen Microbiol. 1991 Jun;137(6):1279-84. doi: 10.1099/00221287-137-6-1279.

Abstract

Rhodococcus rhodochrous ATCC 4275 (Nocardia corallina) has a restriction-modification system with the same recognition sequence, methylation site and cleavage site as the SalI restriction-modification system. Both the restriction endonuclease and the DNA-methyltransferase (DNA-MTase) have been partially purified and characterized. The nuclease has requirements of activity similar to SalI, and a native Mr of about 46,000. The DNA-MTase is a protein with an Mr of about 67,000. No DNA homology was detected between the cloned salI restriction-modification genes of Streptomyces albus and R. rhodochrous chromosomal DNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA, Bacterial / metabolism
  • Deoxyribonucleases, Type II Site-Specific / isolation & purification
  • Deoxyribonucleases, Type II Site-Specific / metabolism*
  • Methylation
  • Molecular Sequence Data
  • Molecular Weight
  • Rhodococcus / enzymology*
  • Rhodococcus / genetics
  • Sequence Homology, Nucleic Acid
  • Site-Specific DNA-Methyltransferase (Adenine-Specific) / isolation & purification
  • Site-Specific DNA-Methyltransferase (Adenine-Specific) / metabolism*

Substances

  • DNA, Bacterial
  • DNA modification methylase SalGI
  • Site-Specific DNA-Methyltransferase (Adenine-Specific)
  • Deoxyribonucleases, Type II Site-Specific
  • GTCGAC-specific type II deoxyribonucleases