Abstract
Rhodococcus rhodochrous ATCC 4275 (Nocardia corallina) has a restriction-modification system with the same recognition sequence, methylation site and cleavage site as the SalI restriction-modification system. Both the restriction endonuclease and the DNA-methyltransferase (DNA-MTase) have been partially purified and characterized. The nuclease has requirements of activity similar to SalI, and a native Mr of about 46,000. The DNA-MTase is a protein with an Mr of about 67,000. No DNA homology was detected between the cloned salI restriction-modification genes of Streptomyces albus and R. rhodochrous chromosomal DNA.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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DNA, Bacterial / metabolism
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Deoxyribonucleases, Type II Site-Specific / isolation & purification
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Deoxyribonucleases, Type II Site-Specific / metabolism*
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Methylation
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Molecular Sequence Data
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Molecular Weight
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Rhodococcus / enzymology*
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Rhodococcus / genetics
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Sequence Homology, Nucleic Acid
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Site-Specific DNA-Methyltransferase (Adenine-Specific) / isolation & purification
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Site-Specific DNA-Methyltransferase (Adenine-Specific) / metabolism*
Substances
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DNA, Bacterial
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DNA modification methylase SalGI
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Site-Specific DNA-Methyltransferase (Adenine-Specific)
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Deoxyribonucleases, Type II Site-Specific
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GTCGAC-specific type II deoxyribonucleases