Preparation of cyclohexene isotopologues and stereoisotopomers from benzene

Nature. 2020 May;581(7808):288-293. doi: 10.1038/s41586-020-2268-y. Epub 2020 May 20.

Abstract

The hydrogen isotopes deuterium (D) and tritium (T) have become essential tools in chemistry, biology and medicine1. Beyond their widespread use in spectroscopy, mass spectrometry and mechanistic and pharmacokinetic studies, there has been considerable interest in incorporating deuterium into drug molecules1. Deutetrabenazine, a deuterated drug that is promising for the treatment of Huntington's disease2, was recently approved by the United States' Food and Drug Administration. The deuterium kinetic isotope effect, which compares the rate of a chemical reaction for a compound with that for its deuterated counterpart, can be substantial1,3,4. The strategic replacement of hydrogen with deuterium can affect both the rate of metabolism and the distribution of metabolites for a compound5, improving the efficacy and safety of a drug. The pharmacokinetics of a deuterated compound depends on the location(s) of deuterium. Although methods are available for deuterium incorporation at both early and late stages of the synthesis of a drug6,7, these processes are often unselective and the stereoisotopic purity can be difficult to measure7,8. Here we describe the preparation of stereoselectively deuterated building blocks for pharmaceutical research. As a proof of concept, we demonstrate a four-step conversion of benzene to cyclohexene with varying degrees of deuterium incorporation, via binding to a tungsten complex. Using different combinations of deuterated and proteated acid and hydride reagents, the deuterated positions on the cyclohexene ring can be controlled precisely. In total, 52 unique stereoisotopomers of cyclohexene are available, in the form of ten different isotopologues. This concept can be extended to prepare discrete stereoisotopomers of functionalized cyclohexenes. Such systematic methods for the preparation of pharmacologically active compounds as discrete stereoisotopomers could improve the pharmacological and toxicological properties of drugs and provide mechanistic information related to their distribution and metabolism in the body.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Benzene / chemistry*
  • Chemistry Techniques, Synthetic*
  • Cyclohexenes / chemical synthesis*
  • Cyclohexenes / chemistry*
  • Databases, Chemical
  • Deuterium / chemistry*
  • Kinetics
  • Molecular Structure
  • Pharmaceutical Preparations / chemical synthesis*
  • Pharmaceutical Preparations / chemistry*
  • Stereoisomerism
  • Tetrabenazine / analogs & derivatives
  • Tetrabenazine / chemical synthesis
  • Tetrabenazine / chemistry
  • Tungsten / chemistry

Substances

  • Cyclohexenes
  • Pharmaceutical Preparations
  • cyclohexene
  • Deuterium
  • Benzene
  • deutetrabenazine
  • Tungsten
  • Tetrabenazine