A secreted peptide acts on BIN2-mediated phosphorylation of ARFs to potentiate auxin response during lateral root development

Nat Cell Biol. 2014 Jan;16(1):66-76. doi: 10.1038/ncb2893. Epub 2013 Dec 22.

Abstract

The phytohormone auxin is a key developmental signal in plants. So far, only auxin perception has been described to trigger the release of transcription factors termed Auxin Response Factors (ARFs) from their auxin/indole-3-acetic acid (AUX/IAA) repressor proteins. Here, we show that phosphorylation of ARF7 and ARF19 by BRASSINOSTEROID-insensitive2 (BIN2) can also potentiate auxin signalling output during lateral root organogenesis. BIN2-mediated phosphorylation of ARF7 and ARF19 suppresses their interaction with AUX/IAAs, and subsequently enhances the transcriptional activity to their target genes lateral organ boundaries-domain16 (LBD16) and LBD29. In this context, BIN2 is under the control of the Tracheary element differentiation inhibitory factor (TDIF)-TDIF receptor (TDR) module. TDIF-initiated TDR signalling directly acts on BIN2-mediated ARF phosphorylation, leading to the regulation of auxin signalling during lateral root development. In summary, this study delineates a TDIF-TDR-BIN2 signalling cascade that controls regulation of ARF and AUX/IAA interaction independent of auxin perception during lateral root development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / drug effects
  • Arabidopsis / genetics
  • Arabidopsis / growth & development*
  • Arabidopsis Proteins / chemistry
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Arabidopsis Proteins / pharmacology*
  • DNA, Plant / metabolism
  • Gene Expression Regulation, Plant / drug effects
  • Indoleacetic Acids / pharmacology*
  • Models, Biological
  • Molecular Sequence Data
  • Mutation / genetics
  • Oligopeptides / pharmacology*
  • Phosphorylation / drug effects
  • Plant Roots / drug effects
  • Plant Roots / genetics
  • Plant Roots / growth & development*
  • Protein Binding / drug effects
  • Protein Binding / genetics
  • Protein Kinases / metabolism*
  • Signal Transduction
  • Transcription Factors / chemistry
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Transcription, Genetic / drug effects

Substances

  • ARF19 protein, Arabidopsis
  • ARF7 protein, Arabidopsis
  • Arabidopsis Proteins
  • DNA, Plant
  • Indoleacetic Acids
  • Oligopeptides
  • Transcription Factors
  • tracheary element differentiation inhibitory factor, Arabidopsis
  • Protein Kinases
  • BIN2 protein, Arabidopsis
  • PXY protein, Arabidopsis