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Engineering the Substrate Specificity of Toluene Degrading Enzyme XylM Using Biosensor XylS and Machine Learning.
Ogawa Y, Saito Y, Yamaguchi H, Katsuyama Y, Ohnishi Y. Ogawa Y, et al. ACS Synth Biol. 2023 Feb 17;12(2):572-582. doi: 10.1021/acssynbio.2c00577. Epub 2023 Feb 3. ACS Synth Biol. 2023. PMID: 36734676
We aimed to engineer XylM to improve its conversion efficiency to a non-native substrate, 2,6-xylenol. Wild-type XylMABC slightly converted 2,6-xylenol to 3-methylsalicylic acid, which is the ligand of the transcriptional regulator XylS in P. putida. By locat …
We aimed to engineer XylM to improve its conversion efficiency to a non-native substrate, 2,6-xylenol. Wild-type XylMABC slightly converted …
Bacterial metabolism of para- and meta-xylene: oxidation of a methyl substituent.
Davey JF, Gibson DT. Davey JF, et al. J Bacteriol. 1974 Sep;119(3):923-9. doi: 10.1128/jb.119.3.923-929.1974. J Bacteriol. 1974. PMID: 4850727 Free PMC article.
When Pseudomonas Pxy-82 was grown on succinate in the presence of m-xylene, 3-methylcatechol and 3-methylsalicylic acid were excreted into the culture medium. A pathway is proposed for the initial reactions utilized by Pseudomonas Pxy to oxidize p- and m-xyle …
When Pseudomonas Pxy-82 was grown on succinate in the presence of m-xylene, 3-methylcatechol and 3-methylsalicylic acid …
The complete assignment of the 13C NMR spectra of lasalocid and the sodium salt-complex of the antibiotic.
Seto H, Westley JW, Pitcher RG. Seto H, et al. J Antibiot (Tokyo). 1978 Apr;31(4):289-93. doi: 10.7164/antibiotics.31.289. J Antibiot (Tokyo). 1978. PMID: 659326 Free article.
All thirty-four signals observed in the 13C nmr of both the free acid form (Ia) and sodium salt (Ib) of the polyether antibiotic lasalocid have been assigned. This was achieved using model compounds such as 3-methylsalicylic acid, the retroaldol ketones from …
All thirty-four signals observed in the 13C nmr of both the free acid form (Ia) and sodium salt (Ib) of the polyether antibiotic lasalocid h …
Synthesis and characterisation of tungsten(VI) oxo-salicylate complexes for use in the chemical vapour deposition of self-cleaning films.
Cross WB, Parkin IP, White AJ, Williams DJ. Cross WB, et al. Dalton Trans. 2005 Apr 7;(7):1287-93. doi: 10.1039/b419235d. Epub 2005 Mar 3. Dalton Trans. 2005. PMID: 15782266
Tungsten(VI) oxo-salicylate complexes were prepared in moderate yield (47 to 63%) by the reactions of WOCl4 and two equivalents of either 3-methylsalicylic acid (MesaliH2) or 3,5-di-isopropylsalicylic acid (di-i-PrsaliH2). ...
Tungsten(VI) oxo-salicylate complexes were prepared in moderate yield (47 to 63%) by the reactions of WOCl4 and two equivalents of either …