Transfection of Corynebacterium lilium protoplasts

J Gen Microbiol. 1985 Dec;131(12):3179-83. doi: 10.1099/00221287-131-12-3179.

Abstract

A protoplast transfection system has been developed for a lysine-producing bacterium, Corynebacterium lilium, using the DNA of phage CL31. Phage CL31 is lytic and specific to C. lilium and has a genome of approximately 48 kb. The transfection procedure involves a polyethylene-glycol-mediated introduction of the DNA into lysozyme-treated cells and has a maximum efficiency of 3 X 10(4) transfectants per microgram DNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriophages / genetics*
  • Corynebacterium / genetics*
  • DNA Restriction Enzymes
  • DNA, Viral / genetics
  • Protoplasts*
  • Transfection*

Substances

  • DNA, Viral
  • DNA Restriction Enzymes