Upregulation of the cfiA carbapenemase gene in a Bacteroides fragilis strain by the novel integrative and conjugative element Tn7563

Tore Taksdal Stubhaug; Nermin Zecic; Dagfinn Skaare

doi:10.1016/j.anaerobe.2023.102785

Upregulation of the cfiA carbapenemase gene in a Bacteroides fragilis strain by the novel integrative and conjugative element Tn7563

Anaerobe. 2023 Oct:83:102785. doi: 10.1016/j.anaerobe.2023.102785. Epub 2023 Sep 23.

Authors

Tore Taksdal Stubhaug¹, Nermin Zecic², Dagfinn Skaare²

Affiliations

¹ Vestfold Hospital Trust, Department of Microbiology, Tønsberg, Norway; University of Oslo, Institute of Clinical Medicine, Oslo, Norway. Electronic address: torstu@siv.no.
² Vestfold Hospital Trust, Department of Microbiology, Tønsberg, Norway.

PMID: 37743024
DOI: 10.1016/j.anaerobe.2023.102785

Abstract

We describe Tn7563, a 31,844-bp integrative and conjugative element (ICE) carrying promoters upregulating the cfiA carbapenemase gene in Bacteroides fragilis strain Tbg-22. Excision and circularization of Tn7563 was demonstrated by PCR. Previously, only insertion sequences (IS) have been shown to carry mobile promoters for cfiA.

Keywords: Bacteroides; Carbapenemase; Gene expression; Integrative and conjugative elements.

MeSH terms

Bacterial Infections*
Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Bacteroides Infections*
Bacteroides fragilis / genetics
DNA Transposable Elements
Humans
Up-Regulation
beta-Lactamases / genetics
beta-Lactamases / metabolism

Substances

carbapenemase
Bacterial Proteins
beta-Lactamases
DNA Transposable Elements