Abstract
Induction of the membrane-associated organic solvent efflux system SrpABC of Pseudomonas putida S12 was examined by cloning a 312-bp DNA fragment, containing the srp promoter, in the broad-host-range reporter vector pKRZ-1. Compounds that are capable of inducing expression of the srpABC genes include aromatic and aliphatic solvents and alcohols. General stress conditions such as pH, temperature, NaCl, or the presence of organic acids did not induce srp transcription. Although the solvent efflux pump in P. putida S12 is a member of the resistance-nodulation-cell division family of transporters, the srpABC genes were not induced by antibiotics or heavy metals.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Anti-Bacterial Agents / pharmacology
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Bacterial Proteins / genetics*
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Biological Transport, Active
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Carrier Proteins / genetics*
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Gene Expression Regulation, Bacterial
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Genes, Reporter
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Hydrogen-Ion Concentration
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Lac Operon
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Membrane Transport Proteins*
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Metals, Heavy
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Plasmids
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Promoter Regions, Genetic
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Pseudomonas putida / genetics*
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Pseudomonas putida / growth & development
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Pseudomonas putida / metabolism
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Solvents*
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Temperature
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Transcriptional Activation
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Carrier Proteins
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Membrane Transport Proteins
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Metals, Heavy
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Solvents
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SrpA protein, Pseudomonas
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SrpB protein, Pseudomonas putida
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SrpC protein, Pseudomonas