Genetic analysis of members of the species Oropouche virus and identification of a novel M segment sequence

Natasha L Tilston-Lunel; Joseph Hughes; Gustavo Olszanski Acrani; Daisy E A da Silva; Raimunda S S Azevedo; Sueli G Rodrigues; Pedro F C Vasconcelos; Marcio R T Nunes; Richard M Elliott

doi:10.1099/vir.0.000108

Genetic analysis of members of the species Oropouche virus and identification of a novel M segment sequence

J Gen Virol. 2015 Jul;96(Pt 7):1636-50. doi: 10.1099/vir.0.000108. Epub 2015 Mar 3.

Authors

Natasha L Tilston-Lunel¹, Joseph Hughes², Gustavo Olszanski Acrani³, Daisy E A da Silva⁴, Raimunda S S Azevedo⁵, Sueli G Rodrigues⁵, Pedro F C Vasconcelos⁵, Marcio R T Nunes⁴, Richard M Elliott²

Affiliations

¹ 1MRC-University of Glasgow Centre for Virus Research, 464 Bearsden Road, Glasgow G61 1QH, Scotland, UK 2Biomedical Sciences Research Complex, School of Biology, University of St Andrews, St Andrews KY16 9ST, Scotland, UK.
² 1MRC-University of Glasgow Centre for Virus Research, 464 Bearsden Road, Glasgow G61 1QH, Scotland, UK.
³ 1MRC-University of Glasgow Centre for Virus Research, 464 Bearsden Road, Glasgow G61 1QH, Scotland, UK 3Department of Cell and Molecular Biology, University of Sao Paulo School of Medicine, 3900, Av Bandeirantes, Ribeirão Preto, SP 14049-900, Brazil.
⁴ 4Center for Technological Innovation, Instituto Evandro Chagas, Ananindeua, Brazil.
⁵ 5Department of Arbovirology and Hemorrhagic Fevers, Instituto Evandro Chagas, Ananindeua, Brazil.

Abstract

Oropouche virus (OROV) is a public health threat in South America, and in particular in northern Brazil, causing frequent outbreaks of febrile illness. Using a combination of deep sequencing and Sanger sequencing approaches, we determined the complete genome sequences of eight clinical isolates that were obtained from patient sera during an Oropouche fever outbreak in Amapa state, northern Brazil, in 2009. We also report the complete genome sequences of two OROV reassortants isolatd from two marmosets in Minas Gerais state, south-east Brazil, in 2012 that contained a novel M genome segment. Interestingly, all 10 isolates possessed a 947 nt S segment that lacked 11 residues in the S-segment 3' UTR compared with the recently redetermined Brazilian prototype OROV strain BeAn19991. OROV maybe circulating more widely in Brazil and in the non-human primate population than previously appreciated, and the identification of yet another reassortant highlights the importance of bunyavirus surveillance in South America.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adolescent
Adult
Animals
Brazil
Bunyaviridae Infections / veterinary
Bunyaviridae Infections / virology
Callithrix
Cluster Analysis
Female
Genome, Viral*
Humans
Male
Middle Aged
Molecular Sequence Data
Orthobunyavirus / classification*
Orthobunyavirus / genetics*
Orthobunyavirus / isolation & purification
Phylogeny
RNA, Viral / genetics*
Reassortant Viruses / classification
Reassortant Viruses / genetics
Reassortant Viruses / isolation & purification
Sequence Analysis, DNA
Sequence Homology
Young Adult

Substances

RNA, Viral

Associated data

GENBANK/KP691603
GENBANK/KP691604
GENBANK/KP691605
GENBANK/KP691606
GENBANK/KP691607
GENBANK/KP691608
GENBANK/KP691609
GENBANK/KP691610
GENBANK/KP691611
GENBANK/KP691612
GENBANK/KP691613
GENBANK/KP691614
GENBANK/KP691615
GENBANK/KP691616
GENBANK/KP691617
GENBANK/KP691618
GENBANK/KP691619
GENBANK/KP691620
GENBANK/KP691621
GENBANK/KP691622
GENBANK/KP691623
GENBANK/KP691624
GENBANK/KP691625
GENBANK/KP691626
GENBANK/KP691627
GENBANK/KP691628
GENBANK/KP691629
GENBANK/KP691630
GENBANK/KP691631
GENBANK/KP691632

Abstract

Publication types

MeSH terms

Substances

Associated data

Grants and funding