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Your search for A dual affinity-tag strategy for the expression and purific retrieved no results
A dual affinity-tag strategy for the expression and purification of human linker histone H1.4 in Escherichia coli.
Ryan DP, Tremethick DJ. Ryan DP, et al. Protein Expr Purif. 2016 Apr;120:160-8. doi: 10.1016/j.pep.2015.12.017. Epub 2015 Dec 29. Protein Expr Purif. 2016. PMID: 26739785
An N-terminal Glutathione-S-Transferase tag was used to promote soluble expression and was combined with a C-terminal hexahistidine tag to facilitate a simple non-denaturing two-step affinity chromatography procedure that results in highly pure …
An N-terminal Glutathione-S-Transferase tag was used to promote soluble expression and was combined with a C-terminal h …
Purification and Characterization of DUOX Peroxidase Homology Domains (PHDs).
Meitzler JL. Meitzler JL. Methods Mol Biol. 2019;1982:61-74. doi: 10.1007/978-1-4939-9424-3_4. Methods Mol Biol. 2019. PMID: 31172466
The approach utilizes a baculovirus expression vector in insect cell culture to produce secreted recombinant PHD; an appended C-terminal His(6) affinity tag was found to be crucial for structural stability. Finally, initial characterization of the activity of …
The approach utilizes a baculovirus expression vector in insect cell culture to produce secreted recombinant PHD; an appended C-termi …
Expression and purification of ataxin-1 protein.
Husain-Ponnampalam R, Turnbull V, Tarlac V, Storey E. Husain-Ponnampalam R, et al. J Neurosci Methods. 2010 May 30;189(1):30-5. doi: 10.1016/j.jneumeth.2010.03.008. Epub 2010 Mar 19. J Neurosci Methods. 2010. PMID: 20304006
This ataxin-1 protein was expressed in Escherichia coli as a fusion protein with a GST tag at the N-terminus and a double (His)(6) tag at the C-terminus. The devised dual affinity tag strategy allowed successful purification of the …
This ataxin-1 protein was expressed in Escherichia coli as a fusion protein with a GST tag at the N-terminus and a double (His …
Reprint of: Expression and purification of ataxin-1 protein.
Husain-Ponnampalam R, Turnbull V, Tarlac V, Storey E. Husain-Ponnampalam R, et al. Protein Expr Purif. 2011 Sep 3. doi: 10.1016/j.pep.2011.08.015. Online ahead of print. Protein Expr Purif. 2011. PMID: 21893199
This ataxin-1 protein was expressed in Escherichia coli as a fusion protein with a GST tag at the N-terminus and a double (His)(6) tag at the C-terminus. The devised dual affinity tag strategy allowed successful purification of the …
This ataxin-1 protein was expressed in Escherichia coli as a fusion protein with a GST tag at the N-terminus and a double (His …
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