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A method for extracting DNA from hard tissues for use in forensic identification.
Samsuwan J, Somboonchokepisal T, Akaraputtiporn T, Srimuang T, Phuengsukdaeng P, Suwannarat A, Mutirangura A, Kitkumthorn N. Samsuwan J, et al. Biomed Rep. 2018 Nov;9(5):433-438. doi: 10.3892/br.2018.1148. Epub 2018 Sep 13. Biomed Rep. 2018. PMID: 30402227 Free PMC article.
With deceased and decayed bodies, personal identification is performed using hard tissue DNA, commonly extracted from bone. ...In the present study, porcine teeth and bone samples were exposed to a variety of environments that imitated personal …
With deceased and decayed bodies, personal identification is performed using hard tissue DNA, commonly extrac
DNA recovery and analysis from skeletal material in modern forensic contexts.
Latham KE, Miller JJ. Latham KE, et al. Forensic Sci Res. 2018 Oct 8;4(1):51-59. doi: 10.1080/20961790.2018.1515594. eCollection 2019. Forensic Sci Res. 2018. PMID: 30915417 Free PMC article. Review.
Since bones and teeth are often the only biological materials remaining after exposure to environmental conditions, intense heat, certain traumatic events and in cases where a significant amount of time has passed since the death of the individual, the ability to purify large qua …
Since bones and teeth are often the only biological materials remaining after exposure to environmental conditions, intense heat, certain tr …
Forensic human identification: Investigation into tooth morphotype and DNA extraction methods from teeth.
Heathfield LJ, Haikney TE, Mole CG, Finaughty C, Zachou AM, Gibbon VE. Heathfield LJ, et al. Sci Justice. 2021 Jul;61(4):339-344. doi: 10.1016/j.scijus.2021.05.005. Epub 2021 May 30. Sci Justice. 2021. PMID: 34172122
When a body is decomposed, hard tissues such as teeth may provide the only DNA source for human identification. ...DNA was extracted using both the QIAamp DNA Investigator Kit and the phenol-chloroform method. ...
When a body is decomposed, hard tissues such as teeth may provide the only DNA source for human identification. …
Evaluation of DNA typing as a positive identification method for soft and hard tissues immersed in strong acids.
Robino C, Pazzi M, Di Vella G, Martinelli D, Mazzola L, Ricci U, Testi R, Vincenti M. Robino C, et al. Leg Med (Tokyo). 2015 Nov;17(6):569-75. doi: 10.1016/j.legalmed.2015.07.004. Epub 2015 Jul 14. Leg Med (Tokyo). 2015. PMID: 26195111 Free article.
By means of an animal model, namely porcine samples, we evaluated standard DNA typing as a method for identifying soft (muscle) and hard (bone and teeth) tissues immersed in strong acids (hydrochloric, nitric and sulfuric acid) or in mixtures of acids …
By means of an animal model, namely porcine samples, we evaluated standard DNA typing as a method for identifying soft (muscle …
The auditory ossicles as a DNA source for genetic identification of highly putrefied cadavers.
Schwark T, Modrow JH, Steinmeier E, Poetsch M, Hasse J, Fischer H, von Wurmb-Schwark N. Schwark T, et al. Int J Legal Med. 2015 May;129(3):457-62. doi: 10.1007/s00414-015-1177-3. Epub 2015 Mar 21. Int J Legal Med. 2015. PMID: 25794688
Genetic identification of putrefied bodies is a common task in forensic medicine. ...Since DNA stability is generally higher in hard tissues, bones or teeth are frequently used as DNA source in such cases. ...
Genetic identification of putrefied bodies is a common task in forensic medicine. ...Since DNA stability is gene …
Forensic DNA extraction methods for human hard tissue: A systematic literature review and meta-analysis of technologies and sample type.
Finaughty C, Heathfield LJ, Kemp V, Márquez-Grant N. Finaughty C, et al. Forensic Sci Int Genet. 2023 Mar;63:102818. doi: 10.1016/j.fsigen.2022.102818. Epub 2022 Dec 7. Forensic Sci Int Genet. 2023. PMID: 36502616 Free article. Review.
In this respect, focus has been placed on DNA extraction methodologies for hard tissue samples, as these are the longest surviving. ...Therefore, the aim of this study was to conduct a thorough systematic literature review to identify potential DNA
In this respect, focus has been placed on DNA extraction methodologies for hard tissue samples, as these are the …
Assessing the viability of carious lesions in human identification using STR typing.
Shbair M, Adnan A, Hao P, Liu Y. Shbair M, et al. Sci Prog. 2021 Apr-Jun;104(2):368504211008054. doi: 10.1177/00368504211008054. Sci Prog. 2021. PMID: 33827332 Free article.
Previous forensic identification was mainly dependent on the pulp and the other hard tissues of intact teeth. However, there is high likelihood that only carious teeth can be available for forensic analysis. This study aimed to validate the use …
Previous forensic identification was mainly dependent on the pulp and the other hard tissues of intact teeth. Ho …
A novel approach in personal identification from tissue samples undergone different processes through STR typing.
Staiti N, Di Martino D, Saravo L. Staiti N, et al. Forensic Sci Int. 2004 Dec 2;146 Suppl:S171-3. doi: 10.1016/j.forsciint.2004.09.052. Forensic Sci Int. 2004. PMID: 15639570
Bone is the most resistant tissue in animal body to time depending degradation and putrefaction, but it is often hard to extract DNA from it because of its highly mineralized structure, which makes DNA extraction and/or amplification h
Bone is the most resistant tissue in animal body to time depending degradation and putrefaction, but it is often hard to ex
Practical aspects of DNA-based forensic studies in dentistry.
Muruganandhan J, Sivakumar G. Muruganandhan J, et al. J Forensic Dent Sci. 2011 Jan;3(1):38-45. doi: 10.4103/0975-1475.85295. J Forensic Dent Sci. 2011. PMID: 22022138 Free PMC article.
This ensures a high yield of the required DNA. Hard tissues like teeth require specialized procedures to extract the genetic material. ...This necessitates calibration of the various methods to achieve best results. DNA analysis can provi …
This ensures a high yield of the required DNA. Hard tissues like teeth require specialized procedures to extract
Effectiveness of various methods of DNA isolation from bones and teeth of animals exposed to high temperature.
Grela M, Jakubczak A, Kowalczyk M, Listos P, Gryzińska M. Grela M, et al. J Forensic Leg Med. 2021 Feb;78:102131. doi: 10.1016/j.jflm.2021.102131. Epub 2021 Feb 3. J Forensic Leg Med. 2021. PMID: 33561692
Bones and teeth collected during necropsy of dogs were burnt in a laboratory oven at 400 C (752 F; 673.15 K) for 5, 10, 15, 30, 45 and 60 min. DNA was isolated according to four different protocols, using three commercial kits, i.e. the PrepFiler Forensic DNA
Bones and teeth collected during necropsy of dogs were burnt in a laboratory oven at 400 C (752 F; 673.15 K) for 5, 10, 15, 30, 45 and 60 mi …
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